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Joint non-uniform sampling of all incremented time delays for quicker acquisition in protein relaxation studies
NMR relaxometry plays crucial role in studies of protein dynamics. The measurement of longitudinal and transverse relaxation rates of [Formula: see text] N is the main source of information on backbone motions. However, even the most basic approach exploiting a series of [Formula: see text] N HSQC s...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5504159/ https://www.ncbi.nlm.nih.gov/pubmed/28508110 http://dx.doi.org/10.1007/s10858-017-0115-8 |
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author | Urbańczyk, Mateusz Nowakowski, Michał Koźmiński, Wiktor Kazimierczuk, Krzysztof |
author_facet | Urbańczyk, Mateusz Nowakowski, Michał Koźmiński, Wiktor Kazimierczuk, Krzysztof |
author_sort | Urbańczyk, Mateusz |
collection | PubMed |
description | NMR relaxometry plays crucial role in studies of protein dynamics. The measurement of longitudinal and transverse relaxation rates of [Formula: see text] N is the main source of information on backbone motions. However, even the most basic approach exploiting a series of [Formula: see text] N HSQC spectra can require several hours of measurement time. Standard non-uniform sampling (NUS), i.e. random under-sampling of indirect time domain, typically cannot reduce this by more than 2–4[Formula: see text] due to relatively low “compressibility” of these spectra. In this paper we propose an extension of NUS to relaxation delays. The two-dimensional space of [Formula: see text] /[Formula: see text] is sampled in a way similar to NUS of [Formula: see text] /[Formula: see text] domain in 3D spectra. The signal is also processed in a way similar to that known from 3D NUS spectra i.e. using one of the most popular compressed sensing algorithms, iterative soft thresholding. The 2D Fourier transform matrix is replaced with mixed inverse Laplace-Fourier transform matrix. The peak positions in resulting 3D spectrum are characterized by two frequency coordinates and relaxation rate and thus no additional fitting of exponential curves is required. The method is tested on three globular proteins, providing satisfactory results in a time corresponding to acquisition of two conventional [Formula: see text] N HSQC spectra. |
format | Online Article Text |
id | pubmed-5504159 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-55041592017-07-25 Joint non-uniform sampling of all incremented time delays for quicker acquisition in protein relaxation studies Urbańczyk, Mateusz Nowakowski, Michał Koźmiński, Wiktor Kazimierczuk, Krzysztof J Biomol NMR Article NMR relaxometry plays crucial role in studies of protein dynamics. The measurement of longitudinal and transverse relaxation rates of [Formula: see text] N is the main source of information on backbone motions. However, even the most basic approach exploiting a series of [Formula: see text] N HSQC spectra can require several hours of measurement time. Standard non-uniform sampling (NUS), i.e. random under-sampling of indirect time domain, typically cannot reduce this by more than 2–4[Formula: see text] due to relatively low “compressibility” of these spectra. In this paper we propose an extension of NUS to relaxation delays. The two-dimensional space of [Formula: see text] /[Formula: see text] is sampled in a way similar to NUS of [Formula: see text] /[Formula: see text] domain in 3D spectra. The signal is also processed in a way similar to that known from 3D NUS spectra i.e. using one of the most popular compressed sensing algorithms, iterative soft thresholding. The 2D Fourier transform matrix is replaced with mixed inverse Laplace-Fourier transform matrix. The peak positions in resulting 3D spectrum are characterized by two frequency coordinates and relaxation rate and thus no additional fitting of exponential curves is required. The method is tested on three globular proteins, providing satisfactory results in a time corresponding to acquisition of two conventional [Formula: see text] N HSQC spectra. Springer Netherlands 2017-05-15 2017 /pmc/articles/PMC5504159/ /pubmed/28508110 http://dx.doi.org/10.1007/s10858-017-0115-8 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Article Urbańczyk, Mateusz Nowakowski, Michał Koźmiński, Wiktor Kazimierczuk, Krzysztof Joint non-uniform sampling of all incremented time delays for quicker acquisition in protein relaxation studies |
title | Joint non-uniform sampling of all incremented time delays for quicker acquisition in protein relaxation studies |
title_full | Joint non-uniform sampling of all incremented time delays for quicker acquisition in protein relaxation studies |
title_fullStr | Joint non-uniform sampling of all incremented time delays for quicker acquisition in protein relaxation studies |
title_full_unstemmed | Joint non-uniform sampling of all incremented time delays for quicker acquisition in protein relaxation studies |
title_short | Joint non-uniform sampling of all incremented time delays for quicker acquisition in protein relaxation studies |
title_sort | joint non-uniform sampling of all incremented time delays for quicker acquisition in protein relaxation studies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5504159/ https://www.ncbi.nlm.nih.gov/pubmed/28508110 http://dx.doi.org/10.1007/s10858-017-0115-8 |
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