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Metabolomic Analysis of Alfalfa (Medicago sativa L.) Root-Symbiotic Rhizobia Responses under Alkali Stress
Alkaline salts (e.g., NaHCO(3) and Na(2)CO(3)) causes more severe morphological and physiological damage to plants than neutral salts (e.g., NaCl and Na(2)SO(4)) due to differences in pH. The mechanism by which plants respond to alkali stress is not fully understood, especially in plants having symb...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5504246/ https://www.ncbi.nlm.nih.gov/pubmed/28744296 http://dx.doi.org/10.3389/fpls.2017.01208 |
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author | Song, Tingting Xu, Huihui Sun, Na Jiang, Liu Tian, Pu Yong, Yueyuan Yang, Weiwei Cai, Hua Cui, Guowen |
author_facet | Song, Tingting Xu, Huihui Sun, Na Jiang, Liu Tian, Pu Yong, Yueyuan Yang, Weiwei Cai, Hua Cui, Guowen |
author_sort | Song, Tingting |
collection | PubMed |
description | Alkaline salts (e.g., NaHCO(3) and Na(2)CO(3)) causes more severe morphological and physiological damage to plants than neutral salts (e.g., NaCl and Na(2)SO(4)) due to differences in pH. The mechanism by which plants respond to alkali stress is not fully understood, especially in plants having symbotic relationships such as alfalfa (Medicago sativa L.). Therefore, a study was designed to evaluate the metabolic response of the root-nodule symbiosis in alfalfa under alkali stress using comparative metabolomics. Rhizobium-nodulized (RI group) and non-nodulized (NI group) alfalfa roots were treated with 200 mmol/L NaHCO(3) and, roots samples were analyzed for malondialdehydyde (MDA), proline, glutathione (GSH), superoxide dismutase (SOD), and peroxidase (POD) content. Additionally, metabolite profiling was conducted using gas chromatography combined with time-of-flight mass spectrometry (GC/TOF-MS). Phenotypically, the RI alfalfa exhibited a greater resistance to alkali stress than the NI plants examined. Physiological analysis and metabolic profiling revealed that RI plants accumulated more antioxidants (SOD, POD, GSH), osmolytes (sugar, glycols, proline), organic acids (succinic acid, fumaric acid, and alpha-ketoglutaric acid), and metabolites that are involved in nitrogen fixation. Our pairwise metabolomics comparisons revealed that RI alfalfa plants exhibited a distinct metabolic profile associated with alkali putative tolerance relative to NI alfalfa plants. Data provide new information about the relationship between non-nodulized, rhizobium-nodulized alfalfa and alkali resistance. |
format | Online Article Text |
id | pubmed-5504246 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55042462017-07-25 Metabolomic Analysis of Alfalfa (Medicago sativa L.) Root-Symbiotic Rhizobia Responses under Alkali Stress Song, Tingting Xu, Huihui Sun, Na Jiang, Liu Tian, Pu Yong, Yueyuan Yang, Weiwei Cai, Hua Cui, Guowen Front Plant Sci Plant Science Alkaline salts (e.g., NaHCO(3) and Na(2)CO(3)) causes more severe morphological and physiological damage to plants than neutral salts (e.g., NaCl and Na(2)SO(4)) due to differences in pH. The mechanism by which plants respond to alkali stress is not fully understood, especially in plants having symbotic relationships such as alfalfa (Medicago sativa L.). Therefore, a study was designed to evaluate the metabolic response of the root-nodule symbiosis in alfalfa under alkali stress using comparative metabolomics. Rhizobium-nodulized (RI group) and non-nodulized (NI group) alfalfa roots were treated with 200 mmol/L NaHCO(3) and, roots samples were analyzed for malondialdehydyde (MDA), proline, glutathione (GSH), superoxide dismutase (SOD), and peroxidase (POD) content. Additionally, metabolite profiling was conducted using gas chromatography combined with time-of-flight mass spectrometry (GC/TOF-MS). Phenotypically, the RI alfalfa exhibited a greater resistance to alkali stress than the NI plants examined. Physiological analysis and metabolic profiling revealed that RI plants accumulated more antioxidants (SOD, POD, GSH), osmolytes (sugar, glycols, proline), organic acids (succinic acid, fumaric acid, and alpha-ketoglutaric acid), and metabolites that are involved in nitrogen fixation. Our pairwise metabolomics comparisons revealed that RI alfalfa plants exhibited a distinct metabolic profile associated with alkali putative tolerance relative to NI alfalfa plants. Data provide new information about the relationship between non-nodulized, rhizobium-nodulized alfalfa and alkali resistance. Frontiers Media S.A. 2017-07-11 /pmc/articles/PMC5504246/ /pubmed/28744296 http://dx.doi.org/10.3389/fpls.2017.01208 Text en Copyright © 2017 Song, Xu, Sun, Jiang, Tian, Yong, Yang, Cai and Cui. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Song, Tingting Xu, Huihui Sun, Na Jiang, Liu Tian, Pu Yong, Yueyuan Yang, Weiwei Cai, Hua Cui, Guowen Metabolomic Analysis of Alfalfa (Medicago sativa L.) Root-Symbiotic Rhizobia Responses under Alkali Stress |
title | Metabolomic Analysis of Alfalfa (Medicago sativa L.) Root-Symbiotic Rhizobia Responses under Alkali Stress |
title_full | Metabolomic Analysis of Alfalfa (Medicago sativa L.) Root-Symbiotic Rhizobia Responses under Alkali Stress |
title_fullStr | Metabolomic Analysis of Alfalfa (Medicago sativa L.) Root-Symbiotic Rhizobia Responses under Alkali Stress |
title_full_unstemmed | Metabolomic Analysis of Alfalfa (Medicago sativa L.) Root-Symbiotic Rhizobia Responses under Alkali Stress |
title_short | Metabolomic Analysis of Alfalfa (Medicago sativa L.) Root-Symbiotic Rhizobia Responses under Alkali Stress |
title_sort | metabolomic analysis of alfalfa (medicago sativa l.) root-symbiotic rhizobia responses under alkali stress |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5504246/ https://www.ncbi.nlm.nih.gov/pubmed/28744296 http://dx.doi.org/10.3389/fpls.2017.01208 |
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