2′-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA

[Image: see text] 5-Hydroxymethylcytidine (hm(5)C) was recently identified as a direct metabolite of m(5)C in RNA. We investigated the stability of hm(5)C in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of m(5)C in RNA, namely 2′-...

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Autores principales: Huber, Sabrina M., van Delft, Pieter, Tanpure, Arun, Miska, Eric A., Balasubramanian, Shankar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5504488/
https://www.ncbi.nlm.nih.gov/pubmed/28107630
http://dx.doi.org/10.1021/jacs.6b12180
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author Huber, Sabrina M.
van Delft, Pieter
Tanpure, Arun
Miska, Eric A.
Balasubramanian, Shankar
author_facet Huber, Sabrina M.
van Delft, Pieter
Tanpure, Arun
Miska, Eric A.
Balasubramanian, Shankar
author_sort Huber, Sabrina M.
collection PubMed
description [Image: see text] 5-Hydroxymethylcytidine (hm(5)C) was recently identified as a direct metabolite of m(5)C in RNA. We investigated the stability of hm(5)C in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of m(5)C in RNA, namely 2′-O-methyl-5-hydroxymethylcytidine (hm(5)Cm). Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels and TET-independent formation of this new RNA modification.
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spelling pubmed-55044882017-07-12 2′-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA Huber, Sabrina M. van Delft, Pieter Tanpure, Arun Miska, Eric A. Balasubramanian, Shankar J Am Chem Soc [Image: see text] 5-Hydroxymethylcytidine (hm(5)C) was recently identified as a direct metabolite of m(5)C in RNA. We investigated the stability of hm(5)C in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of m(5)C in RNA, namely 2′-O-methyl-5-hydroxymethylcytidine (hm(5)Cm). Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels and TET-independent formation of this new RNA modification. American Chemical Society 2017-01-20 2017-02-08 /pmc/articles/PMC5504488/ /pubmed/28107630 http://dx.doi.org/10.1021/jacs.6b12180 Text en Copyright © 2017 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Huber, Sabrina M.
van Delft, Pieter
Tanpure, Arun
Miska, Eric A.
Balasubramanian, Shankar
2′-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA
title 2′-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA
title_full 2′-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA
title_fullStr 2′-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA
title_full_unstemmed 2′-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA
title_short 2′-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA
title_sort 2′-o-methyl-5-hydroxymethylcytidine: a second oxidative derivative of 5-methylcytidine in rna
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5504488/
https://www.ncbi.nlm.nih.gov/pubmed/28107630
http://dx.doi.org/10.1021/jacs.6b12180
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