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The Human Immunodeficiency Virus 1 ASP RNA promotes viral latency by recruiting the Polycomb Repressor Complex 2 and promoting nucleosome assembly

Various epigenetic marks at the HIV-1 5′LTR suppress proviral expression and promote latency. Cellular antisense transcripts known as long noncoding RNAs (lncRNAs) recruit the polycomb repressor complex 2 (PRC2) to gene promoters, which catalyzes trimethylation of lysine 27 on histone H3 (H3K27me3),...

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Autores principales: Zapata, Juan C., Campilongo, Federica, Barclay, Robert A., DeMarino, Catherine, Iglesias-Ussel, Maria D., Kashanchi, Fatah, Romerio, Fabio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505171/
https://www.ncbi.nlm.nih.gov/pubmed/28340355
http://dx.doi.org/10.1016/j.virol.2017.03.002
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author Zapata, Juan C.
Campilongo, Federica
Barclay, Robert A.
DeMarino, Catherine
Iglesias-Ussel, Maria D.
Kashanchi, Fatah
Romerio, Fabio
author_facet Zapata, Juan C.
Campilongo, Federica
Barclay, Robert A.
DeMarino, Catherine
Iglesias-Ussel, Maria D.
Kashanchi, Fatah
Romerio, Fabio
author_sort Zapata, Juan C.
collection PubMed
description Various epigenetic marks at the HIV-1 5′LTR suppress proviral expression and promote latency. Cellular antisense transcripts known as long noncoding RNAs (lncRNAs) recruit the polycomb repressor complex 2 (PRC2) to gene promoters, which catalyzes trimethylation of lysine 27 on histone H3 (H3K27me3), thus promoting nucleosome assembly and suppressing gene expression. We found that an HIV-1 antisense transcript expressed from the 3′LTR and encoding the antisense protein ASP promotes proviral latency. Expression of ASP RNA reduced HIV-1 replication in Jurkat cells. Moreover, ASP RNA expression promoted the establishment and maintenance of HIV-1 latency in Jurkat E4 cells. We show that this transcript interacts with and recruits PRC2 to the HIV-1 5′LTR, increasing accumulation of the suppressive epigenetic mark H3K27me3, while reducing RNA Polymerase II and thus proviral transcription. Altogether, our results suggest that the HIV-1 ASP transcript promotes epigenetic silencing of the HIV-1 5′LTR and proviral latency through the PRC2 pathway.
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spelling pubmed-55051712017-07-11 The Human Immunodeficiency Virus 1 ASP RNA promotes viral latency by recruiting the Polycomb Repressor Complex 2 and promoting nucleosome assembly Zapata, Juan C. Campilongo, Federica Barclay, Robert A. DeMarino, Catherine Iglesias-Ussel, Maria D. Kashanchi, Fatah Romerio, Fabio Virology Article Various epigenetic marks at the HIV-1 5′LTR suppress proviral expression and promote latency. Cellular antisense transcripts known as long noncoding RNAs (lncRNAs) recruit the polycomb repressor complex 2 (PRC2) to gene promoters, which catalyzes trimethylation of lysine 27 on histone H3 (H3K27me3), thus promoting nucleosome assembly and suppressing gene expression. We found that an HIV-1 antisense transcript expressed from the 3′LTR and encoding the antisense protein ASP promotes proviral latency. Expression of ASP RNA reduced HIV-1 replication in Jurkat cells. Moreover, ASP RNA expression promoted the establishment and maintenance of HIV-1 latency in Jurkat E4 cells. We show that this transcript interacts with and recruits PRC2 to the HIV-1 5′LTR, increasing accumulation of the suppressive epigenetic mark H3K27me3, while reducing RNA Polymerase II and thus proviral transcription. Altogether, our results suggest that the HIV-1 ASP transcript promotes epigenetic silencing of the HIV-1 5′LTR and proviral latency through the PRC2 pathway. 2017-03-21 2017-06 /pmc/articles/PMC5505171/ /pubmed/28340355 http://dx.doi.org/10.1016/j.virol.2017.03.002 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Zapata, Juan C.
Campilongo, Federica
Barclay, Robert A.
DeMarino, Catherine
Iglesias-Ussel, Maria D.
Kashanchi, Fatah
Romerio, Fabio
The Human Immunodeficiency Virus 1 ASP RNA promotes viral latency by recruiting the Polycomb Repressor Complex 2 and promoting nucleosome assembly
title The Human Immunodeficiency Virus 1 ASP RNA promotes viral latency by recruiting the Polycomb Repressor Complex 2 and promoting nucleosome assembly
title_full The Human Immunodeficiency Virus 1 ASP RNA promotes viral latency by recruiting the Polycomb Repressor Complex 2 and promoting nucleosome assembly
title_fullStr The Human Immunodeficiency Virus 1 ASP RNA promotes viral latency by recruiting the Polycomb Repressor Complex 2 and promoting nucleosome assembly
title_full_unstemmed The Human Immunodeficiency Virus 1 ASP RNA promotes viral latency by recruiting the Polycomb Repressor Complex 2 and promoting nucleosome assembly
title_short The Human Immunodeficiency Virus 1 ASP RNA promotes viral latency by recruiting the Polycomb Repressor Complex 2 and promoting nucleosome assembly
title_sort human immunodeficiency virus 1 asp rna promotes viral latency by recruiting the polycomb repressor complex 2 and promoting nucleosome assembly
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505171/
https://www.ncbi.nlm.nih.gov/pubmed/28340355
http://dx.doi.org/10.1016/j.virol.2017.03.002
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