Structural modeling of protein-RNA complexes using crosslinking of segmentally isotope labeled RNA and MS/MS

Ribonucleoproteins (RNPs) are key regulators of cellular function. We established an efficient approach that combines segmental isotope labeling of RNA with photo-crosslinking and tandem mass spectrometry to localize protein-RNA interactions simultaneously at amino acid and nucleotide resolution. Th...

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Autores principales: Dorn, G., Leitner, A., Boudet, J., Campagne, S., von Schroetter, C., Moursy, A., Aebersold, R., Allain, F. H.-T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505470/
https://www.ncbi.nlm.nih.gov/pubmed/28346450
http://dx.doi.org/10.1038/nmeth.4235
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author Dorn, G.
Leitner, A.
Boudet, J.
Campagne, S.
von Schroetter, C.
Moursy, A.
Aebersold, R.
Allain, F. H.-T.
author_facet Dorn, G.
Leitner, A.
Boudet, J.
Campagne, S.
von Schroetter, C.
Moursy, A.
Aebersold, R.
Allain, F. H.-T.
author_sort Dorn, G.
collection PubMed
description Ribonucleoproteins (RNPs) are key regulators of cellular function. We established an efficient approach that combines segmental isotope labeling of RNA with photo-crosslinking and tandem mass spectrometry to localize protein-RNA interactions simultaneously at amino acid and nucleotide resolution. The approach was tested on Polypyrimidine Tract Binding Protein 1 and U1 small nuclear RNP and the results support integrative atomic-scale structural modeling thus providing mechanistic insights into RNP regulated processes.
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spelling pubmed-55054702017-09-27 Structural modeling of protein-RNA complexes using crosslinking of segmentally isotope labeled RNA and MS/MS Dorn, G. Leitner, A. Boudet, J. Campagne, S. von Schroetter, C. Moursy, A. Aebersold, R. Allain, F. H.-T. Nat Methods Article Ribonucleoproteins (RNPs) are key regulators of cellular function. We established an efficient approach that combines segmental isotope labeling of RNA with photo-crosslinking and tandem mass spectrometry to localize protein-RNA interactions simultaneously at amino acid and nucleotide resolution. The approach was tested on Polypyrimidine Tract Binding Protein 1 and U1 small nuclear RNP and the results support integrative atomic-scale structural modeling thus providing mechanistic insights into RNP regulated processes. 2017-03-27 2017-05 /pmc/articles/PMC5505470/ /pubmed/28346450 http://dx.doi.org/10.1038/nmeth.4235 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Dorn, G.
Leitner, A.
Boudet, J.
Campagne, S.
von Schroetter, C.
Moursy, A.
Aebersold, R.
Allain, F. H.-T.
Structural modeling of protein-RNA complexes using crosslinking of segmentally isotope labeled RNA and MS/MS
title Structural modeling of protein-RNA complexes using crosslinking of segmentally isotope labeled RNA and MS/MS
title_full Structural modeling of protein-RNA complexes using crosslinking of segmentally isotope labeled RNA and MS/MS
title_fullStr Structural modeling of protein-RNA complexes using crosslinking of segmentally isotope labeled RNA and MS/MS
title_full_unstemmed Structural modeling of protein-RNA complexes using crosslinking of segmentally isotope labeled RNA and MS/MS
title_short Structural modeling of protein-RNA complexes using crosslinking of segmentally isotope labeled RNA and MS/MS
title_sort structural modeling of protein-rna complexes using crosslinking of segmentally isotope labeled rna and ms/ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505470/
https://www.ncbi.nlm.nih.gov/pubmed/28346450
http://dx.doi.org/10.1038/nmeth.4235
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