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Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells

We previously reported that the soft coral-derived bioactive substance, sinuleptolide, can inhibit the proliferation of oral cancer cells in association with oxidative stress. The functional role of oxidative stress in the cell-killing effect of sinuleptolide on oral cancer cells was not investigate...

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Autores principales: Chang, Yung-Ting, Huang, Chiung-Yao, Tang, Jen-Yang, Liaw, Chih-Chuang, Li, Ruei-Nian, Liu, Jing-Ru, Sheu, Jyh-Horng, Chang, Hsueh-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505647/
https://www.ncbi.nlm.nih.gov/pubmed/28740404
http://dx.doi.org/10.2147/OTT.S138123
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author Chang, Yung-Ting
Huang, Chiung-Yao
Tang, Jen-Yang
Liaw, Chih-Chuang
Li, Ruei-Nian
Liu, Jing-Ru
Sheu, Jyh-Horng
Chang, Hsueh-Wei
author_facet Chang, Yung-Ting
Huang, Chiung-Yao
Tang, Jen-Yang
Liaw, Chih-Chuang
Li, Ruei-Nian
Liu, Jing-Ru
Sheu, Jyh-Horng
Chang, Hsueh-Wei
author_sort Chang, Yung-Ting
collection PubMed
description We previously reported that the soft coral-derived bioactive substance, sinuleptolide, can inhibit the proliferation of oral cancer cells in association with oxidative stress. The functional role of oxidative stress in the cell-killing effect of sinuleptolide on oral cancer cells was not investigated as yet. To address this question, we introduced the reactive oxygen species (ROS) scavenger (N-acetylcysteine [NAC]) in a pretreatment to evaluate the sinuleptolide-induced changes to cell viability, morphology, intracellular ROS, mitochondrial superoxide, apoptosis, and DNA damage of oral cancer cells (Ca9-22). After sinuleptolide treatment, antiproliferation, apoptosis-like morphology, ROS/mitochondrial superoxide generation, annexin V-based apoptosis, and γH2AX-based DNA damage were induced. All these changes were blocked by NAC pretreatment at 4 mM for 1 h. This showed that the cell-killing mechanism of oral cancer cells of sinuleptolide is ROS dependent.
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spelling pubmed-55056472017-07-24 Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells Chang, Yung-Ting Huang, Chiung-Yao Tang, Jen-Yang Liaw, Chih-Chuang Li, Ruei-Nian Liu, Jing-Ru Sheu, Jyh-Horng Chang, Hsueh-Wei Onco Targets Ther Original Research We previously reported that the soft coral-derived bioactive substance, sinuleptolide, can inhibit the proliferation of oral cancer cells in association with oxidative stress. The functional role of oxidative stress in the cell-killing effect of sinuleptolide on oral cancer cells was not investigated as yet. To address this question, we introduced the reactive oxygen species (ROS) scavenger (N-acetylcysteine [NAC]) in a pretreatment to evaluate the sinuleptolide-induced changes to cell viability, morphology, intracellular ROS, mitochondrial superoxide, apoptosis, and DNA damage of oral cancer cells (Ca9-22). After sinuleptolide treatment, antiproliferation, apoptosis-like morphology, ROS/mitochondrial superoxide generation, annexin V-based apoptosis, and γH2AX-based DNA damage were induced. All these changes were blocked by NAC pretreatment at 4 mM for 1 h. This showed that the cell-killing mechanism of oral cancer cells of sinuleptolide is ROS dependent. Dove Medical Press 2017-07-04 /pmc/articles/PMC5505647/ /pubmed/28740404 http://dx.doi.org/10.2147/OTT.S138123 Text en © 2017 Chang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Chang, Yung-Ting
Huang, Chiung-Yao
Tang, Jen-Yang
Liaw, Chih-Chuang
Li, Ruei-Nian
Liu, Jing-Ru
Sheu, Jyh-Horng
Chang, Hsueh-Wei
Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells
title Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells
title_full Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells
title_fullStr Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells
title_full_unstemmed Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells
title_short Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells
title_sort reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and dna damage in oral cancer cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505647/
https://www.ncbi.nlm.nih.gov/pubmed/28740404
http://dx.doi.org/10.2147/OTT.S138123
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