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Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells
We previously reported that the soft coral-derived bioactive substance, sinuleptolide, can inhibit the proliferation of oral cancer cells in association with oxidative stress. The functional role of oxidative stress in the cell-killing effect of sinuleptolide on oral cancer cells was not investigate...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove Medical Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505647/ https://www.ncbi.nlm.nih.gov/pubmed/28740404 http://dx.doi.org/10.2147/OTT.S138123 |
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author | Chang, Yung-Ting Huang, Chiung-Yao Tang, Jen-Yang Liaw, Chih-Chuang Li, Ruei-Nian Liu, Jing-Ru Sheu, Jyh-Horng Chang, Hsueh-Wei |
author_facet | Chang, Yung-Ting Huang, Chiung-Yao Tang, Jen-Yang Liaw, Chih-Chuang Li, Ruei-Nian Liu, Jing-Ru Sheu, Jyh-Horng Chang, Hsueh-Wei |
author_sort | Chang, Yung-Ting |
collection | PubMed |
description | We previously reported that the soft coral-derived bioactive substance, sinuleptolide, can inhibit the proliferation of oral cancer cells in association with oxidative stress. The functional role of oxidative stress in the cell-killing effect of sinuleptolide on oral cancer cells was not investigated as yet. To address this question, we introduced the reactive oxygen species (ROS) scavenger (N-acetylcysteine [NAC]) in a pretreatment to evaluate the sinuleptolide-induced changes to cell viability, morphology, intracellular ROS, mitochondrial superoxide, apoptosis, and DNA damage of oral cancer cells (Ca9-22). After sinuleptolide treatment, antiproliferation, apoptosis-like morphology, ROS/mitochondrial superoxide generation, annexin V-based apoptosis, and γH2AX-based DNA damage were induced. All these changes were blocked by NAC pretreatment at 4 mM for 1 h. This showed that the cell-killing mechanism of oral cancer cells of sinuleptolide is ROS dependent. |
format | Online Article Text |
id | pubmed-5505647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-55056472017-07-24 Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells Chang, Yung-Ting Huang, Chiung-Yao Tang, Jen-Yang Liaw, Chih-Chuang Li, Ruei-Nian Liu, Jing-Ru Sheu, Jyh-Horng Chang, Hsueh-Wei Onco Targets Ther Original Research We previously reported that the soft coral-derived bioactive substance, sinuleptolide, can inhibit the proliferation of oral cancer cells in association with oxidative stress. The functional role of oxidative stress in the cell-killing effect of sinuleptolide on oral cancer cells was not investigated as yet. To address this question, we introduced the reactive oxygen species (ROS) scavenger (N-acetylcysteine [NAC]) in a pretreatment to evaluate the sinuleptolide-induced changes to cell viability, morphology, intracellular ROS, mitochondrial superoxide, apoptosis, and DNA damage of oral cancer cells (Ca9-22). After sinuleptolide treatment, antiproliferation, apoptosis-like morphology, ROS/mitochondrial superoxide generation, annexin V-based apoptosis, and γH2AX-based DNA damage were induced. All these changes were blocked by NAC pretreatment at 4 mM for 1 h. This showed that the cell-killing mechanism of oral cancer cells of sinuleptolide is ROS dependent. Dove Medical Press 2017-07-04 /pmc/articles/PMC5505647/ /pubmed/28740404 http://dx.doi.org/10.2147/OTT.S138123 Text en © 2017 Chang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Chang, Yung-Ting Huang, Chiung-Yao Tang, Jen-Yang Liaw, Chih-Chuang Li, Ruei-Nian Liu, Jing-Ru Sheu, Jyh-Horng Chang, Hsueh-Wei Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells |
title | Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells |
title_full | Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells |
title_fullStr | Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells |
title_full_unstemmed | Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells |
title_short | Reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and DNA damage in oral cancer cells |
title_sort | reactive oxygen species mediate soft corals-derived sinuleptolide-induced antiproliferation and dna damage in oral cancer cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505647/ https://www.ncbi.nlm.nih.gov/pubmed/28740404 http://dx.doi.org/10.2147/OTT.S138123 |
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