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Local Application of Statins Significantly Reduced Hypertrophic Scarring in a Rabbit Ear Model

BACKGROUND: We previously showed that intradermal injection of statins is a successful treatment for hypertrophic scarring. Topical application has many advantages over intradermal injection. In this study, we demonstrate the efficacy of topical statin treatment in reducing scar in our validated rab...

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Autores principales: Jia, Shengxian, Xie, Ping, Hong, Seok J., Galiano, Robert D., Mustoe, Thomas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505822/
https://www.ncbi.nlm.nih.gov/pubmed/28740761
http://dx.doi.org/10.1097/GOX.0000000000001294
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author Jia, Shengxian
Xie, Ping
Hong, Seok J.
Galiano, Robert D.
Mustoe, Thomas A.
author_facet Jia, Shengxian
Xie, Ping
Hong, Seok J.
Galiano, Robert D.
Mustoe, Thomas A.
author_sort Jia, Shengxian
collection PubMed
description BACKGROUND: We previously showed that intradermal injection of statins is a successful treatment for hypertrophic scarring. Topical application has many advantages over intradermal injection. In this study, we demonstrate the efficacy of topical statin treatment in reducing scar in our validated rabbit ear scar model. METHODS: Twenty New Zealand White rabbits were divided into 2 study groups, with 6 rabbits receiving 10 μm pravastatin intradermally at postoperative days 15, 18, and 21, and 14 rabbits receiving 0.4%, 2%, and 10% simvastatin topical application at postoperative days 14–25. Four or 6 full-thickness circular dermal punches 7 mm in diameter were made on the ventral surface of the ear down to but not including the perichondrium. Specimens were collected at 28 days to evaluate the effects of statins on hypertrophic scarring. RESULTS: Treatment with pravastatin intradermal administration significantly reduced scarring in terms of scar elevation index. Topical treatment with both medium- and high-dose simvastatin also significantly reduced scarring. High-dose simvastatin topical treatment showed a major effect in scar reduction but induced side effects of scaling, erythema, and epidermal hyperplasia, which were improved with coapplication of cholesterol. There is a dose response in scar reduction with low-, medium- and high-dose simvastatin topical treatment. High-dose simvastatin treatment significantly reduced the messenger ribonucleic acid (mRNA) expression of connective tissue growth factor, consistent with our previously published work on intradermally injected statins. More directly, high-dose simvastatin treatment also significantly reduced the mRNA expression of collagen 1A1. CONCLUSIONS: Topical simvastatin significantly reduces scar formation. The mechanism of efficacy for statin treatment through interference with connective tissue growth factor mRNA expression was confirmed.
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spelling pubmed-55058222017-07-24 Local Application of Statins Significantly Reduced Hypertrophic Scarring in a Rabbit Ear Model Jia, Shengxian Xie, Ping Hong, Seok J. Galiano, Robert D. Mustoe, Thomas A. Plast Reconstr Surg Glob Open Experimental BACKGROUND: We previously showed that intradermal injection of statins is a successful treatment for hypertrophic scarring. Topical application has many advantages over intradermal injection. In this study, we demonstrate the efficacy of topical statin treatment in reducing scar in our validated rabbit ear scar model. METHODS: Twenty New Zealand White rabbits were divided into 2 study groups, with 6 rabbits receiving 10 μm pravastatin intradermally at postoperative days 15, 18, and 21, and 14 rabbits receiving 0.4%, 2%, and 10% simvastatin topical application at postoperative days 14–25. Four or 6 full-thickness circular dermal punches 7 mm in diameter were made on the ventral surface of the ear down to but not including the perichondrium. Specimens were collected at 28 days to evaluate the effects of statins on hypertrophic scarring. RESULTS: Treatment with pravastatin intradermal administration significantly reduced scarring in terms of scar elevation index. Topical treatment with both medium- and high-dose simvastatin also significantly reduced scarring. High-dose simvastatin topical treatment showed a major effect in scar reduction but induced side effects of scaling, erythema, and epidermal hyperplasia, which were improved with coapplication of cholesterol. There is a dose response in scar reduction with low-, medium- and high-dose simvastatin topical treatment. High-dose simvastatin treatment significantly reduced the messenger ribonucleic acid (mRNA) expression of connective tissue growth factor, consistent with our previously published work on intradermally injected statins. More directly, high-dose simvastatin treatment also significantly reduced the mRNA expression of collagen 1A1. CONCLUSIONS: Topical simvastatin significantly reduces scar formation. The mechanism of efficacy for statin treatment through interference with connective tissue growth factor mRNA expression was confirmed. Wolters Kluwer Health 2017-06-14 /pmc/articles/PMC5505822/ /pubmed/28740761 http://dx.doi.org/10.1097/GOX.0000000000001294 Text en Copyright © 2017 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
spellingShingle Experimental
Jia, Shengxian
Xie, Ping
Hong, Seok J.
Galiano, Robert D.
Mustoe, Thomas A.
Local Application of Statins Significantly Reduced Hypertrophic Scarring in a Rabbit Ear Model
title Local Application of Statins Significantly Reduced Hypertrophic Scarring in a Rabbit Ear Model
title_full Local Application of Statins Significantly Reduced Hypertrophic Scarring in a Rabbit Ear Model
title_fullStr Local Application of Statins Significantly Reduced Hypertrophic Scarring in a Rabbit Ear Model
title_full_unstemmed Local Application of Statins Significantly Reduced Hypertrophic Scarring in a Rabbit Ear Model
title_short Local Application of Statins Significantly Reduced Hypertrophic Scarring in a Rabbit Ear Model
title_sort local application of statins significantly reduced hypertrophic scarring in a rabbit ear model
topic Experimental
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505822/
https://www.ncbi.nlm.nih.gov/pubmed/28740761
http://dx.doi.org/10.1097/GOX.0000000000001294
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