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Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation

Piriformospora indica is an endophytic fungus of Sebacinaceae which colonizes the roots of many plant species and confers benefits to the hosts. We demonstrate that approximately 75% of the genes, which respond to P. indica in Arabidopsis roots, differ among seedlings grown on normal phosphate (Pi)...

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Autores principales: Bakshi, Madhunita, Sherameti, Irena, Meichsner, Doreen, Thürich, Johannes, Varma, Ajit, Johri, Atul K., Yeh, Kai-Wun, Oelmüller, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506084/
https://www.ncbi.nlm.nih.gov/pubmed/28747898
http://dx.doi.org/10.3389/fmicb.2017.01262
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author Bakshi, Madhunita
Sherameti, Irena
Meichsner, Doreen
Thürich, Johannes
Varma, Ajit
Johri, Atul K.
Yeh, Kai-Wun
Oelmüller, Ralf
author_facet Bakshi, Madhunita
Sherameti, Irena
Meichsner, Doreen
Thürich, Johannes
Varma, Ajit
Johri, Atul K.
Yeh, Kai-Wun
Oelmüller, Ralf
author_sort Bakshi, Madhunita
collection PubMed
description Piriformospora indica is an endophytic fungus of Sebacinaceae which colonizes the roots of many plant species and confers benefits to the hosts. We demonstrate that approximately 75% of the genes, which respond to P. indica in Arabidopsis roots, differ among seedlings grown on normal phosphate (Pi) or Pi limitation conditions, and among wild-type and the wrky6 mutant impaired in the regulation of the Pi metabolism. Mapman analyses suggest that the fungus activates different signaling, transport, metabolic and developmental programs in the roots of wild-type and wrky6 seedlings under normal and low Pi conditions. Under low Pi, P. indica promotes growth and Pi uptake of wild-type seedlings, and the stimulatory effects are identical for mutants impaired in the PHOSPHATE TRANSPORTERS1;1, -1;2 and -1;4. The data suggest that the fungus does not stimulate Pi uptake, but adapts the expression profiles to Pi limitation in Pi metabolism mutants.
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spelling pubmed-55060842017-07-26 Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation Bakshi, Madhunita Sherameti, Irena Meichsner, Doreen Thürich, Johannes Varma, Ajit Johri, Atul K. Yeh, Kai-Wun Oelmüller, Ralf Front Microbiol Microbiology Piriformospora indica is an endophytic fungus of Sebacinaceae which colonizes the roots of many plant species and confers benefits to the hosts. We demonstrate that approximately 75% of the genes, which respond to P. indica in Arabidopsis roots, differ among seedlings grown on normal phosphate (Pi) or Pi limitation conditions, and among wild-type and the wrky6 mutant impaired in the regulation of the Pi metabolism. Mapman analyses suggest that the fungus activates different signaling, transport, metabolic and developmental programs in the roots of wild-type and wrky6 seedlings under normal and low Pi conditions. Under low Pi, P. indica promotes growth and Pi uptake of wild-type seedlings, and the stimulatory effects are identical for mutants impaired in the PHOSPHATE TRANSPORTERS1;1, -1;2 and -1;4. The data suggest that the fungus does not stimulate Pi uptake, but adapts the expression profiles to Pi limitation in Pi metabolism mutants. Frontiers Media S.A. 2017-07-12 /pmc/articles/PMC5506084/ /pubmed/28747898 http://dx.doi.org/10.3389/fmicb.2017.01262 Text en Copyright © 2017 Bakshi, Sherameti, Meichsner, Thürich, Varma, Johri, Yeh and Oelmüller. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Bakshi, Madhunita
Sherameti, Irena
Meichsner, Doreen
Thürich, Johannes
Varma, Ajit
Johri, Atul K.
Yeh, Kai-Wun
Oelmüller, Ralf
Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation
title Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation
title_full Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation
title_fullStr Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation
title_full_unstemmed Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation
title_short Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation
title_sort piriformospora indica reprograms gene expression in arabidopsis phosphate metabolism mutants but does not compensate for phosphate limitation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506084/
https://www.ncbi.nlm.nih.gov/pubmed/28747898
http://dx.doi.org/10.3389/fmicb.2017.01262
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