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Mitochondrial thioredoxin reductase 2 is elevated in long‐lived primate as well as rodent species and extends fly mean lifespan
In a survey of enzymes related to protein oxidation and cellular redox state, we found activity of the redox enzyme thioredoxin reductase (TXNRD) to be elevated in cells from long‐lived species of rodents, primates, and birds. Elevated TXNRD activity in long‐lived species reflected increases in the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506402/ https://www.ncbi.nlm.nih.gov/pubmed/28474396 http://dx.doi.org/10.1111/acel.12596 |
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author | Pickering, Andrew M. Lehr, Marcus Gendron, Christi M. Pletcher, Scott D. Miller, Richard A. |
author_facet | Pickering, Andrew M. Lehr, Marcus Gendron, Christi M. Pletcher, Scott D. Miller, Richard A. |
author_sort | Pickering, Andrew M. |
collection | PubMed |
description | In a survey of enzymes related to protein oxidation and cellular redox state, we found activity of the redox enzyme thioredoxin reductase (TXNRD) to be elevated in cells from long‐lived species of rodents, primates, and birds. Elevated TXNRD activity in long‐lived species reflected increases in the mitochondrial form, TXNRD2, rather than the cytosolic forms TXNRD1 and TXNRD3. Analysis of published RNA‐Seq data showed elevated TXNRD2 mRNA in multiple organs of longer‐lived primates, suggesting that the phenomenon is not limited to skin‐derived fibroblasts. Elevation of TXNRD2 activity and protein levels was also noted in liver of three different long‐lived mutant mice, and in normal male mice treated with a drug that extends lifespan in males. Overexpression of mitochondrial TXNRD2 in Drosophila melanogaster extended median (but not maximum) lifespan in female flies with a small lifespan extension in males; in contrast, overexpression of the cytosolic form, TXNRD1, did not produce a lifespan extension. |
format | Online Article Text |
id | pubmed-5506402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55064022017-08-01 Mitochondrial thioredoxin reductase 2 is elevated in long‐lived primate as well as rodent species and extends fly mean lifespan Pickering, Andrew M. Lehr, Marcus Gendron, Christi M. Pletcher, Scott D. Miller, Richard A. Aging Cell Original Articles In a survey of enzymes related to protein oxidation and cellular redox state, we found activity of the redox enzyme thioredoxin reductase (TXNRD) to be elevated in cells from long‐lived species of rodents, primates, and birds. Elevated TXNRD activity in long‐lived species reflected increases in the mitochondrial form, TXNRD2, rather than the cytosolic forms TXNRD1 and TXNRD3. Analysis of published RNA‐Seq data showed elevated TXNRD2 mRNA in multiple organs of longer‐lived primates, suggesting that the phenomenon is not limited to skin‐derived fibroblasts. Elevation of TXNRD2 activity and protein levels was also noted in liver of three different long‐lived mutant mice, and in normal male mice treated with a drug that extends lifespan in males. Overexpression of mitochondrial TXNRD2 in Drosophila melanogaster extended median (but not maximum) lifespan in female flies with a small lifespan extension in males; in contrast, overexpression of the cytosolic form, TXNRD1, did not produce a lifespan extension. John Wiley and Sons Inc. 2017-05-05 2017-08 /pmc/articles/PMC5506402/ /pubmed/28474396 http://dx.doi.org/10.1111/acel.12596 Text en © 2017 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Pickering, Andrew M. Lehr, Marcus Gendron, Christi M. Pletcher, Scott D. Miller, Richard A. Mitochondrial thioredoxin reductase 2 is elevated in long‐lived primate as well as rodent species and extends fly mean lifespan |
title | Mitochondrial thioredoxin reductase 2 is elevated in long‐lived primate as well as rodent species and extends fly mean lifespan |
title_full | Mitochondrial thioredoxin reductase 2 is elevated in long‐lived primate as well as rodent species and extends fly mean lifespan |
title_fullStr | Mitochondrial thioredoxin reductase 2 is elevated in long‐lived primate as well as rodent species and extends fly mean lifespan |
title_full_unstemmed | Mitochondrial thioredoxin reductase 2 is elevated in long‐lived primate as well as rodent species and extends fly mean lifespan |
title_short | Mitochondrial thioredoxin reductase 2 is elevated in long‐lived primate as well as rodent species and extends fly mean lifespan |
title_sort | mitochondrial thioredoxin reductase 2 is elevated in long‐lived primate as well as rodent species and extends fly mean lifespan |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506402/ https://www.ncbi.nlm.nih.gov/pubmed/28474396 http://dx.doi.org/10.1111/acel.12596 |
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