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A cell-penetrating protein designed for bimodal fluorescence and magnetic resonance imaging
Multimodal imaging is a highly desirable biomedical application since it can provide complementary information from each imaging modality. We propose a protein engineering-based strategy for the construction of a bimodal probe for fluorescence and magnetic resonance imaging. A recombinant protein wa...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506616/ https://www.ncbi.nlm.nih.gov/pubmed/28757964 http://dx.doi.org/10.1039/c5sc01925g |
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author | Wu, Qin Cheng, Qinqin Yuan, Siming Qian, Junchao Zhong, Kai Qian, Yinfeng Liu, Yangzhong |
author_facet | Wu, Qin Cheng, Qinqin Yuan, Siming Qian, Junchao Zhong, Kai Qian, Yinfeng Liu, Yangzhong |
author_sort | Wu, Qin |
collection | PubMed |
description | Multimodal imaging is a highly desirable biomedical application since it can provide complementary information from each imaging modality. We propose a protein engineering-based strategy for the construction of a bimodal probe for fluorescence and magnetic resonance imaging. A recombinant protein was generated by the fusion of a supercharged green fluorescence protein (GFP(36+)) with a lanthanide-binding tag (dLBT) that can stably bind two Gd(3+) ions. The GFP(36+)–dLBT fusion protein showed strong fluorescence and exhibited efficient contrast enhancement in magnetic resonance imaging. This protein probe improves the MR relaxation more efficiently than Gd-DTPA (gadopentetate dimeglumine). The superior cell-penetrating activity of GFP(36+) allows the efficient cellular uptake of this fusion protein and it can thus be used as a cellular imaging probe. Dual imaging was conducted in vitro and in mice. This result indicates that the fusion of different functional domains is a feasible approach for making multi-modal imaging agents. |
format | Online Article Text |
id | pubmed-5506616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-55066162017-07-28 A cell-penetrating protein designed for bimodal fluorescence and magnetic resonance imaging Wu, Qin Cheng, Qinqin Yuan, Siming Qian, Junchao Zhong, Kai Qian, Yinfeng Liu, Yangzhong Chem Sci Chemistry Multimodal imaging is a highly desirable biomedical application since it can provide complementary information from each imaging modality. We propose a protein engineering-based strategy for the construction of a bimodal probe for fluorescence and magnetic resonance imaging. A recombinant protein was generated by the fusion of a supercharged green fluorescence protein (GFP(36+)) with a lanthanide-binding tag (dLBT) that can stably bind two Gd(3+) ions. The GFP(36+)–dLBT fusion protein showed strong fluorescence and exhibited efficient contrast enhancement in magnetic resonance imaging. This protein probe improves the MR relaxation more efficiently than Gd-DTPA (gadopentetate dimeglumine). The superior cell-penetrating activity of GFP(36+) allows the efficient cellular uptake of this fusion protein and it can thus be used as a cellular imaging probe. Dual imaging was conducted in vitro and in mice. This result indicates that the fusion of different functional domains is a feasible approach for making multi-modal imaging agents. Royal Society of Chemistry 2015-11-01 2015-08-11 /pmc/articles/PMC5506616/ /pubmed/28757964 http://dx.doi.org/10.1039/c5sc01925g Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by-nc/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0) |
spellingShingle | Chemistry Wu, Qin Cheng, Qinqin Yuan, Siming Qian, Junchao Zhong, Kai Qian, Yinfeng Liu, Yangzhong A cell-penetrating protein designed for bimodal fluorescence and magnetic resonance imaging |
title | A cell-penetrating protein designed for bimodal fluorescence and magnetic resonance imaging
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title_full | A cell-penetrating protein designed for bimodal fluorescence and magnetic resonance imaging
|
title_fullStr | A cell-penetrating protein designed for bimodal fluorescence and magnetic resonance imaging
|
title_full_unstemmed | A cell-penetrating protein designed for bimodal fluorescence and magnetic resonance imaging
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title_short | A cell-penetrating protein designed for bimodal fluorescence and magnetic resonance imaging
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title_sort | cell-penetrating protein designed for bimodal fluorescence and magnetic resonance imaging |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506616/ https://www.ncbi.nlm.nih.gov/pubmed/28757964 http://dx.doi.org/10.1039/c5sc01925g |
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