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Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves

The seeds of many nondomesticated plant species synthesize oils containing high amounts of a single unusual fatty acid, many of which have potential usage in industry. Despite the identification of enzymes for unusual oxidized fatty acid synthesis, the production of these fatty acids in engineered s...

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Autores principales: Yurchenko, Olga, Shockey, Jay M., Gidda, Satinder K., Silver, Maxwell I., Chapman, Kent D., Mullen, Robert T., Dyer, John M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506653/
https://www.ncbi.nlm.nih.gov/pubmed/28083898
http://dx.doi.org/10.1111/pbi.12695
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author Yurchenko, Olga
Shockey, Jay M.
Gidda, Satinder K.
Silver, Maxwell I.
Chapman, Kent D.
Mullen, Robert T.
Dyer, John M.
author_facet Yurchenko, Olga
Shockey, Jay M.
Gidda, Satinder K.
Silver, Maxwell I.
Chapman, Kent D.
Mullen, Robert T.
Dyer, John M.
author_sort Yurchenko, Olga
collection PubMed
description The seeds of many nondomesticated plant species synthesize oils containing high amounts of a single unusual fatty acid, many of which have potential usage in industry. Despite the identification of enzymes for unusual oxidized fatty acid synthesis, the production of these fatty acids in engineered seeds remains low and is often hampered by their inefficient exclusion from phospholipids. Recent studies have established the feasibility of increasing triacylglycerol content in plant leaves, which provides a novel approach for increasing energy density of biomass crops. Here, we determined whether the fatty acid composition of leaf oil could be engineered to accumulate unusual fatty acids. Eleostearic acid (ESA) is a conjugated fatty acid produced in seeds of the tung tree (Vernicia fordii) and has both industrial and nutritional end‐uses. Arabidopsis thaliana lines with elevated leaf oil were first generated by transforming wild‐type, cgi‐58 or pxa1 mutants (the latter two of which contain mutations disrupting fatty acid breakdown) with the diacylglycerol acyltransferases (DGAT1 or DGAT2) and/or oleosin genes from tung. High‐leaf‐oil plant lines were then transformed with tung FADX, which encodes the fatty acid desaturase/conjugase responsible for ESA synthesis. Analysis of lipids in leaves revealed that ESA was efficiently excluded from phospholipids, and co‐expression of tung FADX and DGAT2 promoted a synergistic increase in leaf oil content and ESA accumulation. Taken together, these results provide a new approach for increasing leaf oil content that is coupled with accumulation of unusual fatty acids. Implications for production of biofuels, bioproducts, and plant–pest interactions are discussed.
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spelling pubmed-55066532017-08-02 Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves Yurchenko, Olga Shockey, Jay M. Gidda, Satinder K. Silver, Maxwell I. Chapman, Kent D. Mullen, Robert T. Dyer, John M. Plant Biotechnol J Research Articles The seeds of many nondomesticated plant species synthesize oils containing high amounts of a single unusual fatty acid, many of which have potential usage in industry. Despite the identification of enzymes for unusual oxidized fatty acid synthesis, the production of these fatty acids in engineered seeds remains low and is often hampered by their inefficient exclusion from phospholipids. Recent studies have established the feasibility of increasing triacylglycerol content in plant leaves, which provides a novel approach for increasing energy density of biomass crops. Here, we determined whether the fatty acid composition of leaf oil could be engineered to accumulate unusual fatty acids. Eleostearic acid (ESA) is a conjugated fatty acid produced in seeds of the tung tree (Vernicia fordii) and has both industrial and nutritional end‐uses. Arabidopsis thaliana lines with elevated leaf oil were first generated by transforming wild‐type, cgi‐58 or pxa1 mutants (the latter two of which contain mutations disrupting fatty acid breakdown) with the diacylglycerol acyltransferases (DGAT1 or DGAT2) and/or oleosin genes from tung. High‐leaf‐oil plant lines were then transformed with tung FADX, which encodes the fatty acid desaturase/conjugase responsible for ESA synthesis. Analysis of lipids in leaves revealed that ESA was efficiently excluded from phospholipids, and co‐expression of tung FADX and DGAT2 promoted a synergistic increase in leaf oil content and ESA accumulation. Taken together, these results provide a new approach for increasing leaf oil content that is coupled with accumulation of unusual fatty acids. Implications for production of biofuels, bioproducts, and plant–pest interactions are discussed. John Wiley and Sons Inc. 2017-03-15 2017-08 /pmc/articles/PMC5506653/ /pubmed/28083898 http://dx.doi.org/10.1111/pbi.12695 Text en © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Yurchenko, Olga
Shockey, Jay M.
Gidda, Satinder K.
Silver, Maxwell I.
Chapman, Kent D.
Mullen, Robert T.
Dyer, John M.
Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves
title Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves
title_full Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves
title_fullStr Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves
title_full_unstemmed Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves
title_short Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves
title_sort engineering the production of conjugated fatty acids in arabidopsis thaliana leaves
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506653/
https://www.ncbi.nlm.nih.gov/pubmed/28083898
http://dx.doi.org/10.1111/pbi.12695
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