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Study of infectious diseases in archaeological bone material – A dataset

Bones of human and ground sloth remains were analyzed for presence of Trypanosoma cruzi by conventional PCR using primers TC, TC1 and TC2. Sequence results amplified a fragment with the same product size as the primers (300 and 350pb). Amplified PCR product was sequenced and analyzed on GenBank, usi...

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Detalles Bibliográficos
Autores principales: Pucu, Elisa, Cascardo, Paula, Chame, Marcia, Felice, Gisele, Guidon, Niéde, Cleonice Vergne, Maria, Campos, Guadalupe, Roberto Machado-Silva, José, Leles, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506867/
https://www.ncbi.nlm.nih.gov/pubmed/28725674
http://dx.doi.org/10.1016/j.dib.2017.06.054
Descripción
Sumario:Bones of human and ground sloth remains were analyzed for presence of Trypanosoma cruzi by conventional PCR using primers TC, TC1 and TC2. Sequence results amplified a fragment with the same product size as the primers (300 and 350pb). Amplified PCR product was sequenced and analyzed on GenBank, using Blast. Although these sequences did not match with these parasites they showed high amplification with species of bacteria. This article presents the methodology used and the alignment of the sequences. The display of this dataset will allow further analysis of our results and discussion presented in the manuscript “Finding the unexpected: a critical view on molecular diagnosis of infectious diseases in archaeological samples” (Pucu et al. 2017) [1].