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Study of infectious diseases in archaeological bone material – A dataset
Bones of human and ground sloth remains were analyzed for presence of Trypanosoma cruzi by conventional PCR using primers TC, TC1 and TC2. Sequence results amplified a fragment with the same product size as the primers (300 and 350pb). Amplified PCR product was sequenced and analyzed on GenBank, usi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506867/ https://www.ncbi.nlm.nih.gov/pubmed/28725674 http://dx.doi.org/10.1016/j.dib.2017.06.054 |
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author | Pucu, Elisa Cascardo, Paula Chame, Marcia Felice, Gisele Guidon, Niéde Cleonice Vergne, Maria Campos, Guadalupe Roberto Machado-Silva, José Leles, Daniela |
author_facet | Pucu, Elisa Cascardo, Paula Chame, Marcia Felice, Gisele Guidon, Niéde Cleonice Vergne, Maria Campos, Guadalupe Roberto Machado-Silva, José Leles, Daniela |
author_sort | Pucu, Elisa |
collection | PubMed |
description | Bones of human and ground sloth remains were analyzed for presence of Trypanosoma cruzi by conventional PCR using primers TC, TC1 and TC2. Sequence results amplified a fragment with the same product size as the primers (300 and 350pb). Amplified PCR product was sequenced and analyzed on GenBank, using Blast. Although these sequences did not match with these parasites they showed high amplification with species of bacteria. This article presents the methodology used and the alignment of the sequences. The display of this dataset will allow further analysis of our results and discussion presented in the manuscript “Finding the unexpected: a critical view on molecular diagnosis of infectious diseases in archaeological samples” (Pucu et al. 2017) [1]. |
format | Online Article Text |
id | pubmed-5506867 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-55068672017-07-19 Study of infectious diseases in archaeological bone material – A dataset Pucu, Elisa Cascardo, Paula Chame, Marcia Felice, Gisele Guidon, Niéde Cleonice Vergne, Maria Campos, Guadalupe Roberto Machado-Silva, José Leles, Daniela Data Brief Data Article Bones of human and ground sloth remains were analyzed for presence of Trypanosoma cruzi by conventional PCR using primers TC, TC1 and TC2. Sequence results amplified a fragment with the same product size as the primers (300 and 350pb). Amplified PCR product was sequenced and analyzed on GenBank, using Blast. Although these sequences did not match with these parasites they showed high amplification with species of bacteria. This article presents the methodology used and the alignment of the sequences. The display of this dataset will allow further analysis of our results and discussion presented in the manuscript “Finding the unexpected: a critical view on molecular diagnosis of infectious diseases in archaeological samples” (Pucu et al. 2017) [1]. Elsevier 2017-07-04 /pmc/articles/PMC5506867/ /pubmed/28725674 http://dx.doi.org/10.1016/j.dib.2017.06.054 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Pucu, Elisa Cascardo, Paula Chame, Marcia Felice, Gisele Guidon, Niéde Cleonice Vergne, Maria Campos, Guadalupe Roberto Machado-Silva, José Leles, Daniela Study of infectious diseases in archaeological bone material – A dataset |
title | Study of infectious diseases in archaeological bone material – A dataset |
title_full | Study of infectious diseases in archaeological bone material – A dataset |
title_fullStr | Study of infectious diseases in archaeological bone material – A dataset |
title_full_unstemmed | Study of infectious diseases in archaeological bone material – A dataset |
title_short | Study of infectious diseases in archaeological bone material – A dataset |
title_sort | study of infectious diseases in archaeological bone material – a dataset |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506867/ https://www.ncbi.nlm.nih.gov/pubmed/28725674 http://dx.doi.org/10.1016/j.dib.2017.06.054 |
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