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Study of infectious diseases in archaeological bone material – A dataset

Bones of human and ground sloth remains were analyzed for presence of Trypanosoma cruzi by conventional PCR using primers TC, TC1 and TC2. Sequence results amplified a fragment with the same product size as the primers (300 and 350pb). Amplified PCR product was sequenced and analyzed on GenBank, usi...

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Autores principales: Pucu, Elisa, Cascardo, Paula, Chame, Marcia, Felice, Gisele, Guidon, Niéde, Cleonice Vergne, Maria, Campos, Guadalupe, Roberto Machado-Silva, José, Leles, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506867/
https://www.ncbi.nlm.nih.gov/pubmed/28725674
http://dx.doi.org/10.1016/j.dib.2017.06.054
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author Pucu, Elisa
Cascardo, Paula
Chame, Marcia
Felice, Gisele
Guidon, Niéde
Cleonice Vergne, Maria
Campos, Guadalupe
Roberto Machado-Silva, José
Leles, Daniela
author_facet Pucu, Elisa
Cascardo, Paula
Chame, Marcia
Felice, Gisele
Guidon, Niéde
Cleonice Vergne, Maria
Campos, Guadalupe
Roberto Machado-Silva, José
Leles, Daniela
author_sort Pucu, Elisa
collection PubMed
description Bones of human and ground sloth remains were analyzed for presence of Trypanosoma cruzi by conventional PCR using primers TC, TC1 and TC2. Sequence results amplified a fragment with the same product size as the primers (300 and 350pb). Amplified PCR product was sequenced and analyzed on GenBank, using Blast. Although these sequences did not match with these parasites they showed high amplification with species of bacteria. This article presents the methodology used and the alignment of the sequences. The display of this dataset will allow further analysis of our results and discussion presented in the manuscript “Finding the unexpected: a critical view on molecular diagnosis of infectious diseases in archaeological samples” (Pucu et al. 2017) [1].
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spelling pubmed-55068672017-07-19 Study of infectious diseases in archaeological bone material – A dataset Pucu, Elisa Cascardo, Paula Chame, Marcia Felice, Gisele Guidon, Niéde Cleonice Vergne, Maria Campos, Guadalupe Roberto Machado-Silva, José Leles, Daniela Data Brief Data Article Bones of human and ground sloth remains were analyzed for presence of Trypanosoma cruzi by conventional PCR using primers TC, TC1 and TC2. Sequence results amplified a fragment with the same product size as the primers (300 and 350pb). Amplified PCR product was sequenced and analyzed on GenBank, using Blast. Although these sequences did not match with these parasites they showed high amplification with species of bacteria. This article presents the methodology used and the alignment of the sequences. The display of this dataset will allow further analysis of our results and discussion presented in the manuscript “Finding the unexpected: a critical view on molecular diagnosis of infectious diseases in archaeological samples” (Pucu et al. 2017) [1]. Elsevier 2017-07-04 /pmc/articles/PMC5506867/ /pubmed/28725674 http://dx.doi.org/10.1016/j.dib.2017.06.054 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Pucu, Elisa
Cascardo, Paula
Chame, Marcia
Felice, Gisele
Guidon, Niéde
Cleonice Vergne, Maria
Campos, Guadalupe
Roberto Machado-Silva, José
Leles, Daniela
Study of infectious diseases in archaeological bone material – A dataset
title Study of infectious diseases in archaeological bone material – A dataset
title_full Study of infectious diseases in archaeological bone material – A dataset
title_fullStr Study of infectious diseases in archaeological bone material – A dataset
title_full_unstemmed Study of infectious diseases in archaeological bone material – A dataset
title_short Study of infectious diseases in archaeological bone material – A dataset
title_sort study of infectious diseases in archaeological bone material – a dataset
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506867/
https://www.ncbi.nlm.nih.gov/pubmed/28725674
http://dx.doi.org/10.1016/j.dib.2017.06.054
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