Cargando…
Oviductal epithelial cells selected boar sperm according to their functional characteristics
The interaction of oviductal epithelial cells (OECs) with the spermatozoa has beneficial effects on the sperm functions. The aim of this study is to evaluate the in vitro fertilizing capacity of incubating spermatozoa previously selected by density gradient in OEC and determinate some sperm characte...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507082/ https://www.ncbi.nlm.nih.gov/pubmed/27232850 http://dx.doi.org/10.4103/1008-682X.173936 |
_version_ | 1783249683816120320 |
---|---|
author | López-Úbeda, Rebeca García-Vázquez, Francisco A Gadea, Joaquín Matás, Carmen |
author_facet | López-Úbeda, Rebeca García-Vázquez, Francisco A Gadea, Joaquín Matás, Carmen |
author_sort | López-Úbeda, Rebeca |
collection | PubMed |
description | The interaction of oviductal epithelial cells (OECs) with the spermatozoa has beneficial effects on the sperm functions. The aim of this study is to evaluate the in vitro fertilizing capacity of incubating spermatozoa previously selected by density gradient in OEC and determinate some sperm characteristics that could explain the results obtained. In this study, we assessed in vitro fertilization (IVF), tyrosine phosphorylation, phosphatidylserine translocation, nuclear DNA fragmentation, and chromatin decondensation. Three experimental sperm groups, previously selected by Percoll gradient, were established according to the origin of the sperm used for IVF: (i) W30 group: spermatozoa were incubated with oocytes in the absence of OEC; (ii) NB group: after sperm incubation in OEC, the unbound spermatozoa were incubated with oocytes, in the absence of OEC; and (iii) B group: after sperm incubation with OEC, the bound spermatozoa were incubated with oocytes in the OEC plates. The results showed that sperm from the NB group led to a lower IVF yield, accompanied by low penetration rates (NB: 19.6%, B: 94.9%, and W30: 62.9%; P < 0.001) and problems of nuclear decondensation. Moreover, higher levels of tyrosine phosphorylation were observed in the NB group compared with the W30 and B groups (NB: 58.7%, B: 2.5%, and W30: 4.5%; P < 0.01). A similar trend was observed in phosphatidylserine translocation (NB: 93.7%, B: 5.7%, and W30: 44.2%; P < 0.01). These results demonstrate that the OEC exerts a rigorous degree of sperm selection, even within an already highly selected population of spermatozoa, and can capture the best functional spermatozoa for fertilization. |
format | Online Article Text |
id | pubmed-5507082 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-55070822017-07-17 Oviductal epithelial cells selected boar sperm according to their functional characteristics López-Úbeda, Rebeca García-Vázquez, Francisco A Gadea, Joaquín Matás, Carmen Asian J Androl Original Article The interaction of oviductal epithelial cells (OECs) with the spermatozoa has beneficial effects on the sperm functions. The aim of this study is to evaluate the in vitro fertilizing capacity of incubating spermatozoa previously selected by density gradient in OEC and determinate some sperm characteristics that could explain the results obtained. In this study, we assessed in vitro fertilization (IVF), tyrosine phosphorylation, phosphatidylserine translocation, nuclear DNA fragmentation, and chromatin decondensation. Three experimental sperm groups, previously selected by Percoll gradient, were established according to the origin of the sperm used for IVF: (i) W30 group: spermatozoa were incubated with oocytes in the absence of OEC; (ii) NB group: after sperm incubation in OEC, the unbound spermatozoa were incubated with oocytes, in the absence of OEC; and (iii) B group: after sperm incubation with OEC, the bound spermatozoa were incubated with oocytes in the OEC plates. The results showed that sperm from the NB group led to a lower IVF yield, accompanied by low penetration rates (NB: 19.6%, B: 94.9%, and W30: 62.9%; P < 0.001) and problems of nuclear decondensation. Moreover, higher levels of tyrosine phosphorylation were observed in the NB group compared with the W30 and B groups (NB: 58.7%, B: 2.5%, and W30: 4.5%; P < 0.01). A similar trend was observed in phosphatidylserine translocation (NB: 93.7%, B: 5.7%, and W30: 44.2%; P < 0.01). These results demonstrate that the OEC exerts a rigorous degree of sperm selection, even within an already highly selected population of spermatozoa, and can capture the best functional spermatozoa for fertilization. Medknow Publications & Media Pvt Ltd 2017 2016-05-27 /pmc/articles/PMC5507082/ /pubmed/27232850 http://dx.doi.org/10.4103/1008-682X.173936 Text en Copyright: © The Author(s)(2017) http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article López-Úbeda, Rebeca García-Vázquez, Francisco A Gadea, Joaquín Matás, Carmen Oviductal epithelial cells selected boar sperm according to their functional characteristics |
title | Oviductal epithelial cells selected boar sperm according to their functional characteristics |
title_full | Oviductal epithelial cells selected boar sperm according to their functional characteristics |
title_fullStr | Oviductal epithelial cells selected boar sperm according to their functional characteristics |
title_full_unstemmed | Oviductal epithelial cells selected boar sperm according to their functional characteristics |
title_short | Oviductal epithelial cells selected boar sperm according to their functional characteristics |
title_sort | oviductal epithelial cells selected boar sperm according to their functional characteristics |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507082/ https://www.ncbi.nlm.nih.gov/pubmed/27232850 http://dx.doi.org/10.4103/1008-682X.173936 |
work_keys_str_mv | AT lopezubedarebeca oviductalepithelialcellsselectedboarspermaccordingtotheirfunctionalcharacteristics AT garciavazquezfranciscoa oviductalepithelialcellsselectedboarspermaccordingtotheirfunctionalcharacteristics AT gadeajoaquin oviductalepithelialcellsselectedboarspermaccordingtotheirfunctionalcharacteristics AT matascarmen oviductalepithelialcellsselectedboarspermaccordingtotheirfunctionalcharacteristics |