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Oviductal epithelial cells selected boar sperm according to their functional characteristics

The interaction of oviductal epithelial cells (OECs) with the spermatozoa has beneficial effects on the sperm functions. The aim of this study is to evaluate the in vitro fertilizing capacity of incubating spermatozoa previously selected by density gradient in OEC and determinate some sperm characte...

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Autores principales: López-Úbeda, Rebeca, García-Vázquez, Francisco A, Gadea, Joaquín, Matás, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507082/
https://www.ncbi.nlm.nih.gov/pubmed/27232850
http://dx.doi.org/10.4103/1008-682X.173936
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author López-Úbeda, Rebeca
García-Vázquez, Francisco A
Gadea, Joaquín
Matás, Carmen
author_facet López-Úbeda, Rebeca
García-Vázquez, Francisco A
Gadea, Joaquín
Matás, Carmen
author_sort López-Úbeda, Rebeca
collection PubMed
description The interaction of oviductal epithelial cells (OECs) with the spermatozoa has beneficial effects on the sperm functions. The aim of this study is to evaluate the in vitro fertilizing capacity of incubating spermatozoa previously selected by density gradient in OEC and determinate some sperm characteristics that could explain the results obtained. In this study, we assessed in vitro fertilization (IVF), tyrosine phosphorylation, phosphatidylserine translocation, nuclear DNA fragmentation, and chromatin decondensation. Three experimental sperm groups, previously selected by Percoll gradient, were established according to the origin of the sperm used for IVF: (i) W30 group: spermatozoa were incubated with oocytes in the absence of OEC; (ii) NB group: after sperm incubation in OEC, the unbound spermatozoa were incubated with oocytes, in the absence of OEC; and (iii) B group: after sperm incubation with OEC, the bound spermatozoa were incubated with oocytes in the OEC plates. The results showed that sperm from the NB group led to a lower IVF yield, accompanied by low penetration rates (NB: 19.6%, B: 94.9%, and W30: 62.9%; P < 0.001) and problems of nuclear decondensation. Moreover, higher levels of tyrosine phosphorylation were observed in the NB group compared with the W30 and B groups (NB: 58.7%, B: 2.5%, and W30: 4.5%; P < 0.01). A similar trend was observed in phosphatidylserine translocation (NB: 93.7%, B: 5.7%, and W30: 44.2%; P < 0.01). These results demonstrate that the OEC exerts a rigorous degree of sperm selection, even within an already highly selected population of spermatozoa, and can capture the best functional spermatozoa for fertilization.
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spelling pubmed-55070822017-07-17 Oviductal epithelial cells selected boar sperm according to their functional characteristics López-Úbeda, Rebeca García-Vázquez, Francisco A Gadea, Joaquín Matás, Carmen Asian J Androl Original Article The interaction of oviductal epithelial cells (OECs) with the spermatozoa has beneficial effects on the sperm functions. The aim of this study is to evaluate the in vitro fertilizing capacity of incubating spermatozoa previously selected by density gradient in OEC and determinate some sperm characteristics that could explain the results obtained. In this study, we assessed in vitro fertilization (IVF), tyrosine phosphorylation, phosphatidylserine translocation, nuclear DNA fragmentation, and chromatin decondensation. Three experimental sperm groups, previously selected by Percoll gradient, were established according to the origin of the sperm used for IVF: (i) W30 group: spermatozoa were incubated with oocytes in the absence of OEC; (ii) NB group: after sperm incubation in OEC, the unbound spermatozoa were incubated with oocytes, in the absence of OEC; and (iii) B group: after sperm incubation with OEC, the bound spermatozoa were incubated with oocytes in the OEC plates. The results showed that sperm from the NB group led to a lower IVF yield, accompanied by low penetration rates (NB: 19.6%, B: 94.9%, and W30: 62.9%; P < 0.001) and problems of nuclear decondensation. Moreover, higher levels of tyrosine phosphorylation were observed in the NB group compared with the W30 and B groups (NB: 58.7%, B: 2.5%, and W30: 4.5%; P < 0.01). A similar trend was observed in phosphatidylserine translocation (NB: 93.7%, B: 5.7%, and W30: 44.2%; P < 0.01). These results demonstrate that the OEC exerts a rigorous degree of sperm selection, even within an already highly selected population of spermatozoa, and can capture the best functional spermatozoa for fertilization. Medknow Publications & Media Pvt Ltd 2017 2016-05-27 /pmc/articles/PMC5507082/ /pubmed/27232850 http://dx.doi.org/10.4103/1008-682X.173936 Text en Copyright: © The Author(s)(2017) http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
López-Úbeda, Rebeca
García-Vázquez, Francisco A
Gadea, Joaquín
Matás, Carmen
Oviductal epithelial cells selected boar sperm according to their functional characteristics
title Oviductal epithelial cells selected boar sperm according to their functional characteristics
title_full Oviductal epithelial cells selected boar sperm according to their functional characteristics
title_fullStr Oviductal epithelial cells selected boar sperm according to their functional characteristics
title_full_unstemmed Oviductal epithelial cells selected boar sperm according to their functional characteristics
title_short Oviductal epithelial cells selected boar sperm according to their functional characteristics
title_sort oviductal epithelial cells selected boar sperm according to their functional characteristics
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507082/
https://www.ncbi.nlm.nih.gov/pubmed/27232850
http://dx.doi.org/10.4103/1008-682X.173936
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