Cargando…

Improved high sensitivity screen for Huntington disease using a one-step triplet-primed PCR and melting curve assay

Molecular diagnosis of Huntington disease (HD) is currently performed by fluorescent repeat-flanking or triplet-primed PCR (TP-PCR) with capillary electrophoresis (CE). However, CE requires multiple post-PCR steps and may result in high cost in high-throughput settings. We previously described a cos...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zhao, Mingjue, Cheah, Felicia S. H., Chen, Min, Lee, Caroline G., Law, Hai-Yang, Chong, Samuel S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507316/ https://www.ncbi.nlm.nih.gov/pubmed/28700716 http://dx.doi.org/10.1371/journal.pone.0180984

Ejemplares similares

Triplet-primed PCR and Melting Curve Analysis for Rapid Molecular Screening of Spinocerebellar Ataxia Types 1, 2, and 3
por: Lian, Mulias, et al.
Publicado: (2023)

Robust Preimplantation Genetic Testing of Huntington Disease by Combined Triplet-Primed PCR Analysis of the HTT CAG Repeat and Multi-Microsatellite Haplotyping
por: Zhao, Mingjue, et al.
Publicado: (2019)

Simplified strategy for rapid first-line screening of fragile X syndrome: closed-tube triplet-primed PCR and amplicon melt peak analysis
por: Rajan-Babu, Indhu-Shree, et al.
Publicado: (2015)

Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis
por: Sarkar, Shovon Lal, et al.
Publicado: (2022)

A Novel Triplet-Primed PCR Assay to Detect the Full Range of Trinucleotide CAG Repeats in the Huntingtin Gene (HTT)
por: De Luca, Alessandro, et al.
Publicado: (2021)

KRAS genotyping by digital PCR combined with melting curve analysis
por: Tanaka, Junko, et al.
Publicado: (2019)

A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses
por: Wan, Zhenzhou, et al.
Publicado: (2016)

Detection of large expansions in myotonic dystrophy type 1 using triplet primed PCR
por: Singh, Susmita, et al.
Publicado: (2014)

Eprobe Mediated Real-Time PCR Monitoring and Melting Curve Analysis
por: Hanami, Takeshi, et al.
Publicado: (2013)

A novel multiplex PCR for virus detection by melting curve analysis
por: Liu, Pengcheng, et al.
Publicado: (2018)

A simple one-step PCR assay for SNP detection
por: Chen, Jian, et al.
Publicado: (2021)

Use of DNA melting simulation software for in silico diagnostic assay design: targeting regions with complex melting curves and confirmation by real-time PCR using intercalating dyes
por: Rasmussen, John P, et al.
Publicado: (2007)

Development of a Sensitive and Specific Novel qPCR Assay for Simultaneous Detection and Differentiation of Mucormycosis and Aspergillosis by Melting Curve Analysis
por: Pandey, Mragnayani, et al.
Publicado: (2022)

Development of a Melting-Curve-Based Multiplex Real-Time PCR Assay for the Simultaneous Detection of Viruses Causing Respiratory Infection
por: Tavares, Eliandro Reis, et al.
Publicado: (2023)

Single-channel multiplexing without melting curve analysis in real-time PCR
por: Lee, Young-Jo, et al.
Publicado: (2014)

Rapid plasmid replicon typing by real time PCR melting curve analysis
por: Boot, Maikel, et al.
Publicado: (2013)

Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants
por: Juul, Sebastian, et al.
Publicado: (2022)

Performance of a novel melting curve-based qPCR assay for malaria parasites in routine clinical practice in non-endemic setting
por: van Bergen, Kim J. M., et al.
Publicado: (2023)

A base-calling algorithm for Tm-shifted melting curve SNP assay
por: Liang, Kung-Hao, et al.
Publicado: (2011)

Silicon µPCR Chip for Forensic STR Profiling with Hybeacon Probe Melting Curves
por: Cornelis, Senne, et al.
Publicado: (2019)

Practical Prediction of Ten Common Streptococcus pneumoniae Serotypes/Serogroups in One PCR Reaction by Multiplex Ligation-Dependent Probe Amplification and Melting Curve (MLPA-MC) Assay in Shenzhen, China
por: Wu, Lijuan, et al.
Publicado: (2015)

Accuracy and Performance Evaluation of Triplet Repeat Primed PCR as an Alternative to Conventional Diagnostic Methods for Fragile X Syndrome
por: Gu, Hyunjung, et al.
Publicado: (2021)

Trojan triplets: RNA-based pathomechanisms for muscle dysfunction in Huntington’s disease
por: Skov, Martin, et al.
Publicado: (2017)

Detection of Herpes Simplex and Varicella-Zoster Virus in Clinical Specimens by Multiplex Real-Time PCR and Melting Curve Analysis
por: Hong, Yun Ji, et al.
Publicado: (2014)

Novel probe-based melting curve assays for the characterization of fluoroquinolone resistance in Mycoplasma genitalium
por: Tickner, Jacob A., et al.
Publicado: (2022)

Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR
por: Lorente, Aiala, et al.
Publicado: (2008)

PCR Multiplexing Based on a Single Fluorescent Channel Using Dynamic Melting Curve Analysis
por: Zhang, Haoqing, et al.
Publicado: (2020)

New rapid one-step PCR diagnostic assay for Plasmodium falciparum infective mosquitoes
por: Kefi, Mary, et al.
Publicado: (2018)

Quantification of Hantaan Virus with a SYBR Green Ⅰ-Based One-Step qRT-PCR Assay
por: Jiang, Wei, et al.
Publicado: (2013)

A real-time ARMS PCR/high-resolution melt curve assay for the detection of the three primary mitochondrial mutations in Leber’s hereditary optic neuropathy
por: Ryan, Siobhan Eustace, et al.
Publicado: (2016)

Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides
por: Otaguiri, Eliane Saori, et al.
Publicado: (2018)

Identification of Novel Microsatellite Markers Flanking the SMN1 and SMN2 Duplicated Region and Inclusion Into a Single-Tube Tridecaplex Panel for Haplotype-Based Preimplantation Genetic Testing of Spinal Muscular Atrophy
por: Zhao, Mingjue, et al.
Publicado: (2019)

Use of the melting curve assay as a means for high-throughput quantification of Illumina sequencing libraries
por: Shinozuka, Hiroshi, et al.
Publicado: (2016)

Melting Curve-based Assay as an Alternative Technique for the Accurate Detection of SARS-CoV-2
por: Aboutalebian, Shima, et al.
Publicado: (2022)

Simultaneous detection of Fusarium culmorum and F. graminearum in plant material by duplex PCR with melting curve analysis
por: Brandfass, Christoph, et al.
Publicado: (2006)

COLD-PCR enhanced melting curve analysis improves diagnostic accuracy for KRAS mutations in colorectal carcinoma
por: Pritchard, Colin C, et al.
Publicado: (2010)

Differentiation of Campylobacter jejuni and Campylobacter coli Using Multiplex-PCR and High Resolution Melt Curve Analysis
por: Banowary, Banya, et al.
Publicado: (2015)

Identification and quantification of virulence factors of enterotoxigenic Escherichia coli by high-resolution melting curve quantitative PCR
por: Wang, Weilan, et al.
Publicado: (2017)

A Multiplex PCR Melting-Curve-Analysis-Based Detection Method for the Discrimination of Five Aspergillus Species
por: Tokamani, Maria, et al.
Publicado: (2023)

Rapid detection of the irinotecan‐related UGT1A1*28 polymorphism by asymmetric PCR melting curve analysis using one fluorescent probe
por: Kong, Xiaomu, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_hcn69901sbujc468iv0fej4ju9