Cargando…
Pooling strategy and chromosome painting characterize a living zebroid for the first time
We have investigated the complex karyotype of a living zebra-donkey hybrid for the first time using chromosome-specific painting probes produced from flow-sorted chromosomes from a zebra (Equus burchelli) and horse (Equus caballus). As the chromosomes proved difficult to distinguish from one another...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507506/ https://www.ncbi.nlm.nih.gov/pubmed/28700625 http://dx.doi.org/10.1371/journal.pone.0180158 |
_version_ | 1783249748690468864 |
---|---|
author | Iannuzzi, Alessandra Pereira, Jorge Iannuzzi, Clara Fu, Beiyuan Ferguson-Smith, Malcolm |
author_facet | Iannuzzi, Alessandra Pereira, Jorge Iannuzzi, Clara Fu, Beiyuan Ferguson-Smith, Malcolm |
author_sort | Iannuzzi, Alessandra |
collection | PubMed |
description | We have investigated the complex karyotype of a living zebra-donkey hybrid for the first time using chromosome-specific painting probes produced from flow-sorted chromosomes from a zebra (Equus burchelli) and horse (Equus caballus). As the chromosomes proved difficult to distinguish from one another, a successful new strategy was devised to resolve the difficulty and characterize each chromosome. This was based on selecting five panels of whole chromosome painting probes that could differentiate zebra and donkey chromosomes by labelling the probes with either FITC or Cy3 fluorochromes. Each panel was hybridized sequentially to the same G-Q-banded metaphases and the results combined so that every zebra and donkey chromosome in each suitable metaphase could be identified. A diploid number of 2n = 53, XY was found, containing haploid sets of 22 chromosomes from the zebra and 31 chromosomes from the donkey, without evidence of chromosome rearrangement. This new strategy, developed for the first time, may have several applications in the resolution of other complex hybrid karyotypes and chromosomal aberrations. |
format | Online Article Text |
id | pubmed-5507506 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-55075062017-07-25 Pooling strategy and chromosome painting characterize a living zebroid for the first time Iannuzzi, Alessandra Pereira, Jorge Iannuzzi, Clara Fu, Beiyuan Ferguson-Smith, Malcolm PLoS One Research Article We have investigated the complex karyotype of a living zebra-donkey hybrid for the first time using chromosome-specific painting probes produced from flow-sorted chromosomes from a zebra (Equus burchelli) and horse (Equus caballus). As the chromosomes proved difficult to distinguish from one another, a successful new strategy was devised to resolve the difficulty and characterize each chromosome. This was based on selecting five panels of whole chromosome painting probes that could differentiate zebra and donkey chromosomes by labelling the probes with either FITC or Cy3 fluorochromes. Each panel was hybridized sequentially to the same G-Q-banded metaphases and the results combined so that every zebra and donkey chromosome in each suitable metaphase could be identified. A diploid number of 2n = 53, XY was found, containing haploid sets of 22 chromosomes from the zebra and 31 chromosomes from the donkey, without evidence of chromosome rearrangement. This new strategy, developed for the first time, may have several applications in the resolution of other complex hybrid karyotypes and chromosomal aberrations. Public Library of Science 2017-07-12 /pmc/articles/PMC5507506/ /pubmed/28700625 http://dx.doi.org/10.1371/journal.pone.0180158 Text en © 2017 Iannuzzi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Iannuzzi, Alessandra Pereira, Jorge Iannuzzi, Clara Fu, Beiyuan Ferguson-Smith, Malcolm Pooling strategy and chromosome painting characterize a living zebroid for the first time |
title | Pooling strategy and chromosome painting characterize a living zebroid for the first time |
title_full | Pooling strategy and chromosome painting characterize a living zebroid for the first time |
title_fullStr | Pooling strategy and chromosome painting characterize a living zebroid for the first time |
title_full_unstemmed | Pooling strategy and chromosome painting characterize a living zebroid for the first time |
title_short | Pooling strategy and chromosome painting characterize a living zebroid for the first time |
title_sort | pooling strategy and chromosome painting characterize a living zebroid for the first time |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507506/ https://www.ncbi.nlm.nih.gov/pubmed/28700625 http://dx.doi.org/10.1371/journal.pone.0180158 |
work_keys_str_mv | AT iannuzzialessandra poolingstrategyandchromosomepaintingcharacterizealivingzebroidforthefirsttime AT pereirajorge poolingstrategyandchromosomepaintingcharacterizealivingzebroidforthefirsttime AT iannuzziclara poolingstrategyandchromosomepaintingcharacterizealivingzebroidforthefirsttime AT fubeiyuan poolingstrategyandchromosomepaintingcharacterizealivingzebroidforthefirsttime AT fergusonsmithmalcolm poolingstrategyandchromosomepaintingcharacterizealivingzebroidforthefirsttime |