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Amplification of overlapping DNA amplicons in a single-tube multiplex PCR for targeted next-generation sequencing of BRCA1 and BRCA2

Current PCR-based target enrichment methods for next generation sequencing (NGS) of overlapping amplicons often requires separate PCR reactions and subsequent pooling of amplicons from the different reactions. The study presents a novel method, deemed stem-loop inhibition mediated amplification (SLI...

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Detalles Bibliográficos
Autores principales:	Schenk, Desiree, Song, Gang, Ke, Yue, Wang, Zhaohui
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507532/ https://www.ncbi.nlm.nih.gov/pubmed/28704513 http://dx.doi.org/10.1371/journal.pone.0181062

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