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Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human
Human Corneal epithelial stem cells (CESCs) have been identified to reside in limbus for more than 2 decades. However, the precise location of CESCs in other mammalian remains elusive. This study was to identify differential localization of putative CESCs in mice. Through a series of murine corneal...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507988/ https://www.ncbi.nlm.nih.gov/pubmed/28701781 http://dx.doi.org/10.1038/s41598-017-04569-w |
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author | Li, Jin Xiao, Yangyan Coursey, Terry G. Chen, Xin Deng, Ruzhi Lu, Fan Pflugfelder, Stephen C. Li, De-Quan |
author_facet | Li, Jin Xiao, Yangyan Coursey, Terry G. Chen, Xin Deng, Ruzhi Lu, Fan Pflugfelder, Stephen C. Li, De-Quan |
author_sort | Li, Jin |
collection | PubMed |
description | Human Corneal epithelial stem cells (CESCs) have been identified to reside in limbus for more than 2 decades. However, the precise location of CESCs in other mammalian remains elusive. This study was to identify differential localization of putative CESCs in mice. Through a series of murine corneal cross-sections from different directions, we identified that anatomically and morphologically the murine limbus is composed of the thinnest epithelium and the thinnest stroma without any palisades of Vogt-like niche structure. The cells expressing five of stem/progenitor cell markers are localized in basal layer of entire murine corneal epithelium. BrdU label-retaining cells, a key characteristic of epithelial stem cells, are detected in both limbal and central cornea of mouse eye. Functionally, corneal epithelium can be regenerated in cultures from central and limbal explants of murine cornea. Such a distribution of mouse CESCs is different from human cornea, where limbal stem cell concept has been well established and accepted. We are aware that some new evidence supports limbal stem cell concept in mouse recently. However, it is important to know that central cornea may provide an alternative source of stem cells when one utilizes mice as animal model for corneal research. |
format | Online Article Text |
id | pubmed-5507988 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55079882017-07-14 Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human Li, Jin Xiao, Yangyan Coursey, Terry G. Chen, Xin Deng, Ruzhi Lu, Fan Pflugfelder, Stephen C. Li, De-Quan Sci Rep Article Human Corneal epithelial stem cells (CESCs) have been identified to reside in limbus for more than 2 decades. However, the precise location of CESCs in other mammalian remains elusive. This study was to identify differential localization of putative CESCs in mice. Through a series of murine corneal cross-sections from different directions, we identified that anatomically and morphologically the murine limbus is composed of the thinnest epithelium and the thinnest stroma without any palisades of Vogt-like niche structure. The cells expressing five of stem/progenitor cell markers are localized in basal layer of entire murine corneal epithelium. BrdU label-retaining cells, a key characteristic of epithelial stem cells, are detected in both limbal and central cornea of mouse eye. Functionally, corneal epithelium can be regenerated in cultures from central and limbal explants of murine cornea. Such a distribution of mouse CESCs is different from human cornea, where limbal stem cell concept has been well established and accepted. We are aware that some new evidence supports limbal stem cell concept in mouse recently. However, it is important to know that central cornea may provide an alternative source of stem cells when one utilizes mice as animal model for corneal research. Nature Publishing Group UK 2017-07-12 /pmc/articles/PMC5507988/ /pubmed/28701781 http://dx.doi.org/10.1038/s41598-017-04569-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Li, Jin Xiao, Yangyan Coursey, Terry G. Chen, Xin Deng, Ruzhi Lu, Fan Pflugfelder, Stephen C. Li, De-Quan Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human |
title | Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human |
title_full | Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human |
title_fullStr | Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human |
title_full_unstemmed | Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human |
title_short | Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human |
title_sort | identification for differential localization of putative corneal epithelial stem cells in mouse and human |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507988/ https://www.ncbi.nlm.nih.gov/pubmed/28701781 http://dx.doi.org/10.1038/s41598-017-04569-w |
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