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Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells

Invasion of periodontal pathogens into periodontal tissues is an important step that can cause tissue destruction in periodontal diseases. Porphyromonas gingivalis is a keystone pathogen and its gingipains are key virulence factors. Fusobacterium nucleatum is a bridge organism that mediates coadhesi...

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Autores principales: Jung, Young-Jung, Jun, Hye-Kyoung, Choi, Bong-Kyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5508355/
https://www.ncbi.nlm.nih.gov/pubmed/28748028
http://dx.doi.org/10.1080/20002297.2017.1320193
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author Jung, Young-Jung
Jun, Hye-Kyoung
Choi, Bong-Kyu
author_facet Jung, Young-Jung
Jun, Hye-Kyoung
Choi, Bong-Kyu
author_sort Jung, Young-Jung
collection PubMed
description Invasion of periodontal pathogens into periodontal tissues is an important step that can cause tissue destruction in periodontal diseases. Porphyromonas gingivalis is a keystone pathogen and its gingipains are key virulence factors. Fusobacterium nucleatum is a bridge organism that mediates coadhesion of disease-causing late colonizers such as P. gingivalis and early colonizers during the development of dental biofilms. The aim of this study was to investigate how P. gingivalis, in particular its gingipains, influences the invasion of coinfecting F. nucleatum into gingival epithelial cells. When invasion of F. nucleatum was analyzed after 4 h of infection, invasion of F. nucleatum was suppressed in the presence of P. gingivalis compared with during monoinfection. However, coinfection with a gingipain-null mutant of P. gingivalis did not affect invasion of F. nucleatum. Inhibition of PI3K reduced invasion of F. nucleatum. P. gingivalis inactivated the PI3K/AKT pathway, which was also dependent on gingipains. Survival of intracellular F. nucleatum was promoted by P. gingivalis with Arg gingipain mutation. The results suggest that P. gingivalis, in particular its gingipains, can affect the invasion of coinfecting F. nucleatum through modulating intracellular signaling of the host cells.
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spelling pubmed-55083552017-07-26 Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells Jung, Young-Jung Jun, Hye-Kyoung Choi, Bong-Kyu J Oral Microbiol Original Article Invasion of periodontal pathogens into periodontal tissues is an important step that can cause tissue destruction in periodontal diseases. Porphyromonas gingivalis is a keystone pathogen and its gingipains are key virulence factors. Fusobacterium nucleatum is a bridge organism that mediates coadhesion of disease-causing late colonizers such as P. gingivalis and early colonizers during the development of dental biofilms. The aim of this study was to investigate how P. gingivalis, in particular its gingipains, influences the invasion of coinfecting F. nucleatum into gingival epithelial cells. When invasion of F. nucleatum was analyzed after 4 h of infection, invasion of F. nucleatum was suppressed in the presence of P. gingivalis compared with during monoinfection. However, coinfection with a gingipain-null mutant of P. gingivalis did not affect invasion of F. nucleatum. Inhibition of PI3K reduced invasion of F. nucleatum. P. gingivalis inactivated the PI3K/AKT pathway, which was also dependent on gingipains. Survival of intracellular F. nucleatum was promoted by P. gingivalis with Arg gingipain mutation. The results suggest that P. gingivalis, in particular its gingipains, can affect the invasion of coinfecting F. nucleatum through modulating intracellular signaling of the host cells. Taylor & Francis 2017-06-12 /pmc/articles/PMC5508355/ /pubmed/28748028 http://dx.doi.org/10.1080/20002297.2017.1320193 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Jung, Young-Jung
Jun, Hye-Kyoung
Choi, Bong-Kyu
Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells
title Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells
title_full Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells
title_fullStr Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells
title_full_unstemmed Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells
title_short Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells
title_sort porphyromonas gingivalis suppresses invasion of fusobacterium nucleatum into gingival epithelial cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5508355/
https://www.ncbi.nlm.nih.gov/pubmed/28748028
http://dx.doi.org/10.1080/20002297.2017.1320193
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