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Biofilm sensitivity of seven Streptococcus mutans strains to different fluoride levels
The effect of fluoride concentrations in dental products could be different depending on the Streptococcus mutans strain. The aim of this study was to investigate the effect of different fluoride concentrations corresponding to dental products on biofilm formation and metabolic activity of S. mutans...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5508399/ https://www.ncbi.nlm.nih.gov/pubmed/28748032 http://dx.doi.org/10.1080/20002297.2017.1328265 |
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author | Nassar, Hani M. Gregory, Richard L. |
author_facet | Nassar, Hani M. Gregory, Richard L. |
author_sort | Nassar, Hani M. |
collection | PubMed |
description | The effect of fluoride concentrations in dental products could be different depending on the Streptococcus mutans strain. The aim of this study was to investigate the effect of different fluoride concentrations corresponding to dental products on biofilm formation and metabolic activity of S. mutans strains. Seven S. mutans strains (UA159, A32-2, NG8, 10449, UA130, LM7, and OMZ175) were inoculated into 96-well microtiter plates and were tested with various concentrations of sodium fluoride (0.0, 1.0, 1.56, 3.13, 6.25, 12.5, 25, 50, 100, 125, 175, 225, 275, 625, 1,250, 2,250, and 5,500 ppm) for inhibition of biofilm formation and bacterial metabolic activity by recording absorbance values followed by scanning electron microscope (SEM) images. Data were analyzed by one-way analysis of variance and Tukey’s tests (α = 5%). Significantly more (p≤0.05) biofilm mass in the presence of fluoride was produced by A32-2 and NG8. UA130, LM7, and OMZ175 were more sensitive to increased fluoride and demonstrated few bacterial cells and extracellular polysaccharide (EPS) production at 100 ppm in SEM images. All strains were unable to produce significant biofilm at concentrations >225 ppm. Patients with tolerantS. mutans strains would potentially benefit less from the inherent antibacterial effect of fluoride. |
format | Online Article Text |
id | pubmed-5508399 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-55083992017-07-26 Biofilm sensitivity of seven Streptococcus mutans strains to different fluoride levels Nassar, Hani M. Gregory, Richard L. J Oral Microbiol Original Article The effect of fluoride concentrations in dental products could be different depending on the Streptococcus mutans strain. The aim of this study was to investigate the effect of different fluoride concentrations corresponding to dental products on biofilm formation and metabolic activity of S. mutans strains. Seven S. mutans strains (UA159, A32-2, NG8, 10449, UA130, LM7, and OMZ175) were inoculated into 96-well microtiter plates and were tested with various concentrations of sodium fluoride (0.0, 1.0, 1.56, 3.13, 6.25, 12.5, 25, 50, 100, 125, 175, 225, 275, 625, 1,250, 2,250, and 5,500 ppm) for inhibition of biofilm formation and bacterial metabolic activity by recording absorbance values followed by scanning electron microscope (SEM) images. Data were analyzed by one-way analysis of variance and Tukey’s tests (α = 5%). Significantly more (p≤0.05) biofilm mass in the presence of fluoride was produced by A32-2 and NG8. UA130, LM7, and OMZ175 were more sensitive to increased fluoride and demonstrated few bacterial cells and extracellular polysaccharide (EPS) production at 100 ppm in SEM images. All strains were unable to produce significant biofilm at concentrations >225 ppm. Patients with tolerantS. mutans strains would potentially benefit less from the inherent antibacterial effect of fluoride. Taylor & Francis 2017-06-12 /pmc/articles/PMC5508399/ /pubmed/28748032 http://dx.doi.org/10.1080/20002297.2017.1328265 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nassar, Hani M. Gregory, Richard L. Biofilm sensitivity of seven Streptococcus mutans strains to different fluoride levels |
title | Biofilm sensitivity of seven Streptococcus mutans strains to different fluoride levels |
title_full | Biofilm sensitivity of seven Streptococcus mutans strains to different fluoride levels |
title_fullStr | Biofilm sensitivity of seven Streptococcus mutans strains to different fluoride levels |
title_full_unstemmed | Biofilm sensitivity of seven Streptococcus mutans strains to different fluoride levels |
title_short | Biofilm sensitivity of seven Streptococcus mutans strains to different fluoride levels |
title_sort | biofilm sensitivity of seven streptococcus mutans strains to different fluoride levels |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5508399/ https://www.ncbi.nlm.nih.gov/pubmed/28748032 http://dx.doi.org/10.1080/20002297.2017.1328265 |
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