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Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates

Integral membrane proteins fold inefficiently and are susceptible to turnover via the endoplasmic reticulum–associated degradation (ERAD) pathway. During ERAD, misfolded proteins are recognized by molecular chaperones, polyubiquitinated, and retrotranslocated to the cytoplasm for proteasomal degrada...

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Autores principales: Guerriero, Christopher J., Reutter, Karl-Richard, Augustine, Andrew A., Preston, G. Michael, Weiberth, Kurt F., Mackie, Timothy D., Cleveland-Rubeor, Hillary C., Bethel, Neville P., Callenberg, Keith M., Nakatsukasa, Kunio, Grabe, Michael, Brodsky, Jeffrey L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509421/
https://www.ncbi.nlm.nih.gov/pubmed/28539401
http://dx.doi.org/10.1091/mbc.E17-03-0184
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author Guerriero, Christopher J.
Reutter, Karl-Richard
Augustine, Andrew A.
Preston, G. Michael
Weiberth, Kurt F.
Mackie, Timothy D.
Cleveland-Rubeor, Hillary C.
Bethel, Neville P.
Callenberg, Keith M.
Nakatsukasa, Kunio
Grabe, Michael
Brodsky, Jeffrey L.
author_facet Guerriero, Christopher J.
Reutter, Karl-Richard
Augustine, Andrew A.
Preston, G. Michael
Weiberth, Kurt F.
Mackie, Timothy D.
Cleveland-Rubeor, Hillary C.
Bethel, Neville P.
Callenberg, Keith M.
Nakatsukasa, Kunio
Grabe, Michael
Brodsky, Jeffrey L.
author_sort Guerriero, Christopher J.
collection PubMed
description Integral membrane proteins fold inefficiently and are susceptible to turnover via the endoplasmic reticulum–associated degradation (ERAD) pathway. During ERAD, misfolded proteins are recognized by molecular chaperones, polyubiquitinated, and retrotranslocated to the cytoplasm for proteasomal degradation. Although many aspects of this pathway are defined, how transmembrane helices (TMHs) are removed from the membrane and into the cytoplasm before degradation is poorly understood. In this study, we asked whether the hydrophobic character of a TMH acts as an energetic barrier to retrotranslocation. To this end, we designed a dual-pass model ERAD substrate, Chimera A*, which contains the cytoplasmic misfolded domain from a characterized ERAD substrate, Sterile 6* (Ste6p*). We found that the degradation requirements for Chimera A* and Ste6p* are similar, but Chimera A* was retrotranslocated more efficiently than Ste6p* in an in vitro assay in which retrotranslocation can be quantified. We then constructed a series of Chimera A* variants containing synthetic TMHs with a range of ΔG values for membrane insertion. TMH hydrophobicity correlated inversely with retrotranslocation efficiency, and in all cases, retrotranslocation remained Cdc48p dependent. These findings provide insight into the energetic restrictions on the retrotranslocation reaction, as well as a new computational approach to predict retrotranslocation efficiency.
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spelling pubmed-55094212017-09-30 Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates Guerriero, Christopher J. Reutter, Karl-Richard Augustine, Andrew A. Preston, G. Michael Weiberth, Kurt F. Mackie, Timothy D. Cleveland-Rubeor, Hillary C. Bethel, Neville P. Callenberg, Keith M. Nakatsukasa, Kunio Grabe, Michael Brodsky, Jeffrey L. Mol Biol Cell Articles Integral membrane proteins fold inefficiently and are susceptible to turnover via the endoplasmic reticulum–associated degradation (ERAD) pathway. During ERAD, misfolded proteins are recognized by molecular chaperones, polyubiquitinated, and retrotranslocated to the cytoplasm for proteasomal degradation. Although many aspects of this pathway are defined, how transmembrane helices (TMHs) are removed from the membrane and into the cytoplasm before degradation is poorly understood. In this study, we asked whether the hydrophobic character of a TMH acts as an energetic barrier to retrotranslocation. To this end, we designed a dual-pass model ERAD substrate, Chimera A*, which contains the cytoplasmic misfolded domain from a characterized ERAD substrate, Sterile 6* (Ste6p*). We found that the degradation requirements for Chimera A* and Ste6p* are similar, but Chimera A* was retrotranslocated more efficiently than Ste6p* in an in vitro assay in which retrotranslocation can be quantified. We then constructed a series of Chimera A* variants containing synthetic TMHs with a range of ΔG values for membrane insertion. TMH hydrophobicity correlated inversely with retrotranslocation efficiency, and in all cases, retrotranslocation remained Cdc48p dependent. These findings provide insight into the energetic restrictions on the retrotranslocation reaction, as well as a new computational approach to predict retrotranslocation efficiency. The American Society for Cell Biology 2017-07-15 /pmc/articles/PMC5509421/ /pubmed/28539401 http://dx.doi.org/10.1091/mbc.E17-03-0184 Text en © 2017 Guerriero et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Guerriero, Christopher J.
Reutter, Karl-Richard
Augustine, Andrew A.
Preston, G. Michael
Weiberth, Kurt F.
Mackie, Timothy D.
Cleveland-Rubeor, Hillary C.
Bethel, Neville P.
Callenberg, Keith M.
Nakatsukasa, Kunio
Grabe, Michael
Brodsky, Jeffrey L.
Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates
title Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates
title_full Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates
title_fullStr Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates
title_full_unstemmed Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates
title_short Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates
title_sort transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane erad substrates
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509421/
https://www.ncbi.nlm.nih.gov/pubmed/28539401
http://dx.doi.org/10.1091/mbc.E17-03-0184
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