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CircRNA_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting miR-630
Although CircRNA_100269 is a biomarker used to predict cancer recurrence, its expression and function in gastric cancer (GC) remain unknown. In this study, the expression of circRNA_100269 and its potential downstream miRNA targets were investigated. The molecular function and regulatory mechanism o...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509457/ https://www.ncbi.nlm.nih.gov/pubmed/28657541 http://dx.doi.org/10.18632/aging.101254 |
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author | Zhang, Yan Liu, Hao Li, Wende Yu, Jiang Li, Jin Shen, Zhiyong Ye, Gentai Qi, Xiaolong Li, Guoxin |
author_facet | Zhang, Yan Liu, Hao Li, Wende Yu, Jiang Li, Jin Shen, Zhiyong Ye, Gentai Qi, Xiaolong Li, Guoxin |
author_sort | Zhang, Yan |
collection | PubMed |
description | Although CircRNA_100269 is a biomarker used to predict cancer recurrence, its expression and function in gastric cancer (GC) remain unknown. In this study, the expression of circRNA_100269 and its potential downstream miRNA targets were investigated. The molecular function and regulatory mechanism of circRNA_100269 in GC cell lines were also elucidated. The expression levels of circRNA_100269 and its linear isomer LPHN2 mRNA were found to be downregulated (p<0.01) in GC tissues. The target miRNA was predicted to be miR-630, whose expression was upregulated (p<0.01) and found to be negatively correlated with that of circRNA_100269 (r = −0.688) in GC tissues. Moreover, direct interaction of circRNA_100269 and miR-630 was confirmed through dual-luciferase assays. Overexpressing the circRNA_100269 plasmid inhibited cell proliferation (p<0.05). Furthermore, transfection of miR-630 mimics into cell lines overexpressing circRNA_100269 blocked the function of circRNA_100269 (p<0.05). Thus, circRNA_100269 level was downregulated in GC and correlated negatively with that of miR-630. Taken together, our results suggest that circRNA_100269 and miR-630 comprise a novel pathway that regulates proliferation of GC cells. |
format | Online Article Text |
id | pubmed-5509457 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-55094572017-08-03 CircRNA_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting miR-630 Zhang, Yan Liu, Hao Li, Wende Yu, Jiang Li, Jin Shen, Zhiyong Ye, Gentai Qi, Xiaolong Li, Guoxin Aging (Albany NY) Research Paper Although CircRNA_100269 is a biomarker used to predict cancer recurrence, its expression and function in gastric cancer (GC) remain unknown. In this study, the expression of circRNA_100269 and its potential downstream miRNA targets were investigated. The molecular function and regulatory mechanism of circRNA_100269 in GC cell lines were also elucidated. The expression levels of circRNA_100269 and its linear isomer LPHN2 mRNA were found to be downregulated (p<0.01) in GC tissues. The target miRNA was predicted to be miR-630, whose expression was upregulated (p<0.01) and found to be negatively correlated with that of circRNA_100269 (r = −0.688) in GC tissues. Moreover, direct interaction of circRNA_100269 and miR-630 was confirmed through dual-luciferase assays. Overexpressing the circRNA_100269 plasmid inhibited cell proliferation (p<0.05). Furthermore, transfection of miR-630 mimics into cell lines overexpressing circRNA_100269 blocked the function of circRNA_100269 (p<0.05). Thus, circRNA_100269 level was downregulated in GC and correlated negatively with that of miR-630. Taken together, our results suggest that circRNA_100269 and miR-630 comprise a novel pathway that regulates proliferation of GC cells. Impact Journals LLC 2017-06-27 /pmc/articles/PMC5509457/ /pubmed/28657541 http://dx.doi.org/10.18632/aging.101254 Text en Copyright: © 2017 Zhang et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Zhang, Yan Liu, Hao Li, Wende Yu, Jiang Li, Jin Shen, Zhiyong Ye, Gentai Qi, Xiaolong Li, Guoxin CircRNA_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting miR-630 |
title | CircRNA_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting miR-630 |
title_full | CircRNA_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting miR-630 |
title_fullStr | CircRNA_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting miR-630 |
title_full_unstemmed | CircRNA_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting miR-630 |
title_short | CircRNA_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting miR-630 |
title_sort | circrna_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting mir-630 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509457/ https://www.ncbi.nlm.nih.gov/pubmed/28657541 http://dx.doi.org/10.18632/aging.101254 |
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