Cargando…

Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence

Groundnut (Arachis hypogaea L.) is an important oil seed crop having major biotic constraint in production due to stem rot disease caused by fungus, Athelia rolfsii causing 25–80% loss in productivity. As chemical and biological combating strategies of this fungus are not very effective, thus genome...

Descripción completa

Detalles Bibliográficos
Autores principales: Iquebal, M. A., Tomar, Rukam S., Parakhia, M. V., Singla, Deepak, Jaiswal, Sarika, Rathod, V. M., Padhiyar, S. M., Kumar, Neeraj, Rai, Anil, Kumar, Dinesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509663/
https://www.ncbi.nlm.nih.gov/pubmed/28706242
http://dx.doi.org/10.1038/s41598-017-05478-8
_version_ 1783250045817061376
author Iquebal, M. A.
Tomar, Rukam S.
Parakhia, M. V.
Singla, Deepak
Jaiswal, Sarika
Rathod, V. M.
Padhiyar, S. M.
Kumar, Neeraj
Rai, Anil
Kumar, Dinesh
author_facet Iquebal, M. A.
Tomar, Rukam S.
Parakhia, M. V.
Singla, Deepak
Jaiswal, Sarika
Rathod, V. M.
Padhiyar, S. M.
Kumar, Neeraj
Rai, Anil
Kumar, Dinesh
author_sort Iquebal, M. A.
collection PubMed
description Groundnut (Arachis hypogaea L.) is an important oil seed crop having major biotic constraint in production due to stem rot disease caused by fungus, Athelia rolfsii causing 25–80% loss in productivity. As chemical and biological combating strategies of this fungus are not very effective, thus genome sequencing can reveal virulence and pathogenicity related genes for better understanding of the host-parasite interaction. We report draft assembly of Athelia rolfsii genome of ~73 Mb having 8919 contigs. Annotation analysis revealed 16830 genes which are involved in fungicide resistance, virulence and pathogenicity along with putative effector and lethal genes. Secretome analysis revealed CAZY genes representing 1085 enzymatic genes, glycoside hydrolases, carbohydrate esterases, carbohydrate-binding modules, auxillary activities, glycosyl transferases and polysaccharide lyases. Repeat analysis revealed 11171 SSRs, LTR, GYPSY and COPIA elements. Comparative analysis with other existing ascomycotina genome predicted conserved domain family of WD40, CYP450, Pkinase and ABC transporter revealing insight of evolution of pathogenicity and virulence. This study would help in understanding pathogenicity and virulence at molecular level and development of new combating strategies. Such approach is imperative in endeavour of genome based solution in stem rot disease management leading to better productivity of groundnut crop in tropical region of world.
format Online
Article
Text
id pubmed-5509663
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-55096632017-07-14 Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence Iquebal, M. A. Tomar, Rukam S. Parakhia, M. V. Singla, Deepak Jaiswal, Sarika Rathod, V. M. Padhiyar, S. M. Kumar, Neeraj Rai, Anil Kumar, Dinesh Sci Rep Article Groundnut (Arachis hypogaea L.) is an important oil seed crop having major biotic constraint in production due to stem rot disease caused by fungus, Athelia rolfsii causing 25–80% loss in productivity. As chemical and biological combating strategies of this fungus are not very effective, thus genome sequencing can reveal virulence and pathogenicity related genes for better understanding of the host-parasite interaction. We report draft assembly of Athelia rolfsii genome of ~73 Mb having 8919 contigs. Annotation analysis revealed 16830 genes which are involved in fungicide resistance, virulence and pathogenicity along with putative effector and lethal genes. Secretome analysis revealed CAZY genes representing 1085 enzymatic genes, glycoside hydrolases, carbohydrate esterases, carbohydrate-binding modules, auxillary activities, glycosyl transferases and polysaccharide lyases. Repeat analysis revealed 11171 SSRs, LTR, GYPSY and COPIA elements. Comparative analysis with other existing ascomycotina genome predicted conserved domain family of WD40, CYP450, Pkinase and ABC transporter revealing insight of evolution of pathogenicity and virulence. This study would help in understanding pathogenicity and virulence at molecular level and development of new combating strategies. Such approach is imperative in endeavour of genome based solution in stem rot disease management leading to better productivity of groundnut crop in tropical region of world. Nature Publishing Group UK 2017-07-13 /pmc/articles/PMC5509663/ /pubmed/28706242 http://dx.doi.org/10.1038/s41598-017-05478-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Iquebal, M. A.
Tomar, Rukam S.
Parakhia, M. V.
Singla, Deepak
Jaiswal, Sarika
Rathod, V. M.
Padhiyar, S. M.
Kumar, Neeraj
Rai, Anil
Kumar, Dinesh
Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence
title Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence
title_full Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence
title_fullStr Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence
title_full_unstemmed Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence
title_short Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence
title_sort draft whole genome sequence of groundnut stem rot fungus athelia rolfsii revealing genetic architect of its pathogenicity and virulence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509663/
https://www.ncbi.nlm.nih.gov/pubmed/28706242
http://dx.doi.org/10.1038/s41598-017-05478-8
work_keys_str_mv AT iquebalma draftwholegenomesequenceofgroundnutstemrotfungusatheliarolfsiirevealinggeneticarchitectofitspathogenicityandvirulence
AT tomarrukams draftwholegenomesequenceofgroundnutstemrotfungusatheliarolfsiirevealinggeneticarchitectofitspathogenicityandvirulence
AT parakhiamv draftwholegenomesequenceofgroundnutstemrotfungusatheliarolfsiirevealinggeneticarchitectofitspathogenicityandvirulence
AT singladeepak draftwholegenomesequenceofgroundnutstemrotfungusatheliarolfsiirevealinggeneticarchitectofitspathogenicityandvirulence
AT jaiswalsarika draftwholegenomesequenceofgroundnutstemrotfungusatheliarolfsiirevealinggeneticarchitectofitspathogenicityandvirulence
AT rathodvm draftwholegenomesequenceofgroundnutstemrotfungusatheliarolfsiirevealinggeneticarchitectofitspathogenicityandvirulence
AT padhiyarsm draftwholegenomesequenceofgroundnutstemrotfungusatheliarolfsiirevealinggeneticarchitectofitspathogenicityandvirulence
AT kumarneeraj draftwholegenomesequenceofgroundnutstemrotfungusatheliarolfsiirevealinggeneticarchitectofitspathogenicityandvirulence
AT raianil draftwholegenomesequenceofgroundnutstemrotfungusatheliarolfsiirevealinggeneticarchitectofitspathogenicityandvirulence
AT kumardinesh draftwholegenomesequenceofgroundnutstemrotfungusatheliarolfsiirevealinggeneticarchitectofitspathogenicityandvirulence