Cargando…

Highly efficient multicolor multifocus microscopy by optimal design of diffraction binary gratings

Multifocus microscopy (MFM) allows sensitive and fast three-dimensional imaging. It relies on the efficient design of diffraction phase gratings yielding homogeneous intensities in desired diffraction orders. Such performances are however guaranteed only for a specific wavelength. Here, we discuss a...

Descripción completa

Detalles Bibliográficos
Autores principales: Hajj, Bassam, Oudjedi, Laura, Fiche, Jean-Bernard, Dahan, Maxime, Nollmann, Marcelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509674/
https://www.ncbi.nlm.nih.gov/pubmed/28706216
http://dx.doi.org/10.1038/s41598-017-05531-6
_version_ 1783250048480444416
author Hajj, Bassam
Oudjedi, Laura
Fiche, Jean-Bernard
Dahan, Maxime
Nollmann, Marcelo
author_facet Hajj, Bassam
Oudjedi, Laura
Fiche, Jean-Bernard
Dahan, Maxime
Nollmann, Marcelo
author_sort Hajj, Bassam
collection PubMed
description Multifocus microscopy (MFM) allows sensitive and fast three-dimensional imaging. It relies on the efficient design of diffraction phase gratings yielding homogeneous intensities in desired diffraction orders. Such performances are however guaranteed only for a specific wavelength. Here, we discuss a novel approach for designing binary phase gratings with dual color properties and improved diffraction efficiency for MFM. We simulate binary diffraction gratings with tunable phase shifts to explore its best diffraction performances. We report the design and fabrication of a binary array generator of 3 × 3 equal-intensity diffraction orders with 74% efficiency, 95% uniformity and dual color capability. The multicolor properties of this new design are highlighted by two-color MFM imaging. Finally, we discuss the basics of extending this approach to a variety of diffraction pattern designs.
format Online
Article
Text
id pubmed-5509674
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-55096742017-07-14 Highly efficient multicolor multifocus microscopy by optimal design of diffraction binary gratings Hajj, Bassam Oudjedi, Laura Fiche, Jean-Bernard Dahan, Maxime Nollmann, Marcelo Sci Rep Article Multifocus microscopy (MFM) allows sensitive and fast three-dimensional imaging. It relies on the efficient design of diffraction phase gratings yielding homogeneous intensities in desired diffraction orders. Such performances are however guaranteed only for a specific wavelength. Here, we discuss a novel approach for designing binary phase gratings with dual color properties and improved diffraction efficiency for MFM. We simulate binary diffraction gratings with tunable phase shifts to explore its best diffraction performances. We report the design and fabrication of a binary array generator of 3 × 3 equal-intensity diffraction orders with 74% efficiency, 95% uniformity and dual color capability. The multicolor properties of this new design are highlighted by two-color MFM imaging. Finally, we discuss the basics of extending this approach to a variety of diffraction pattern designs. Nature Publishing Group UK 2017-07-13 /pmc/articles/PMC5509674/ /pubmed/28706216 http://dx.doi.org/10.1038/s41598-017-05531-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hajj, Bassam
Oudjedi, Laura
Fiche, Jean-Bernard
Dahan, Maxime
Nollmann, Marcelo
Highly efficient multicolor multifocus microscopy by optimal design of diffraction binary gratings
title Highly efficient multicolor multifocus microscopy by optimal design of diffraction binary gratings
title_full Highly efficient multicolor multifocus microscopy by optimal design of diffraction binary gratings
title_fullStr Highly efficient multicolor multifocus microscopy by optimal design of diffraction binary gratings
title_full_unstemmed Highly efficient multicolor multifocus microscopy by optimal design of diffraction binary gratings
title_short Highly efficient multicolor multifocus microscopy by optimal design of diffraction binary gratings
title_sort highly efficient multicolor multifocus microscopy by optimal design of diffraction binary gratings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509674/
https://www.ncbi.nlm.nih.gov/pubmed/28706216
http://dx.doi.org/10.1038/s41598-017-05531-6
work_keys_str_mv AT hajjbassam highlyefficientmulticolormultifocusmicroscopybyoptimaldesignofdiffractionbinarygratings
AT oudjedilaura highlyefficientmulticolormultifocusmicroscopybyoptimaldesignofdiffractionbinarygratings
AT fichejeanbernard highlyefficientmulticolormultifocusmicroscopybyoptimaldesignofdiffractionbinarygratings
AT dahanmaxime highlyefficientmulticolormultifocusmicroscopybyoptimaldesignofdiffractionbinarygratings
AT nollmannmarcelo highlyefficientmulticolormultifocusmicroscopybyoptimaldesignofdiffractionbinarygratings