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Effects of radiofrequency exposure emitted from a GSM mobile phone on proliferation, differentiation, and apoptosis of neural stem cells

Due to the importance of neural stem cells (NSCs) in plasticity of the nervous system and treating neurodegenerative diseases, the main goal of this study was to evaluate the effects of radiofrequency radiation emitted from a GSM 900-MHz mobile phone with different exposure duration on proliferation...

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Autores principales: Eghlidospour, Mahsa, Ghanbari, Amir, Mortazavi, Seyyed Mohammad Javad, Azari, Hassan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Association of Anatomists 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509895/
https://www.ncbi.nlm.nih.gov/pubmed/28713615
http://dx.doi.org/10.5115/acb.2017.50.2.115
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author Eghlidospour, Mahsa
Ghanbari, Amir
Mortazavi, Seyyed Mohammad Javad
Azari, Hassan
author_facet Eghlidospour, Mahsa
Ghanbari, Amir
Mortazavi, Seyyed Mohammad Javad
Azari, Hassan
author_sort Eghlidospour, Mahsa
collection PubMed
description Due to the importance of neural stem cells (NSCs) in plasticity of the nervous system and treating neurodegenerative diseases, the main goal of this study was to evaluate the effects of radiofrequency radiation emitted from a GSM 900-MHz mobile phone with different exposure duration on proliferation, differentiation and apoptosis of adult murine NSCs in vitro. We used neurosphere assay to evaluate NSCs proliferation, and immunofluorescence assay of neural cell markers to examine NSCs differentiation. We also employed alamarBlue and caspase 3 apoptosis assays to assess harmful effects of mobile phone on NSCs. Our results showed that the number and size of resulting neurospheres and also the percentage of cells differentiated into neurons decreased significantly with increasing exposure duration to GSM 900-MHz radiofrequency (RF)-electromagnetic field (EMF). In contrast, exposure to GSM 900-MHz RF-EMF at different durations did not influence cell viability and apoptosis of NSCs and also their astrocytic differentiation. It is concluded that accumulating dose of GSM 900-MHz RF-EMF might have devastating effects on NSCs proliferation and neurogenesis requiring more causations in terms of using mobile devices.
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spelling pubmed-55098952017-07-14 Effects of radiofrequency exposure emitted from a GSM mobile phone on proliferation, differentiation, and apoptosis of neural stem cells Eghlidospour, Mahsa Ghanbari, Amir Mortazavi, Seyyed Mohammad Javad Azari, Hassan Anat Cell Biol Original Article Due to the importance of neural stem cells (NSCs) in plasticity of the nervous system and treating neurodegenerative diseases, the main goal of this study was to evaluate the effects of radiofrequency radiation emitted from a GSM 900-MHz mobile phone with different exposure duration on proliferation, differentiation and apoptosis of adult murine NSCs in vitro. We used neurosphere assay to evaluate NSCs proliferation, and immunofluorescence assay of neural cell markers to examine NSCs differentiation. We also employed alamarBlue and caspase 3 apoptosis assays to assess harmful effects of mobile phone on NSCs. Our results showed that the number and size of resulting neurospheres and also the percentage of cells differentiated into neurons decreased significantly with increasing exposure duration to GSM 900-MHz radiofrequency (RF)-electromagnetic field (EMF). In contrast, exposure to GSM 900-MHz RF-EMF at different durations did not influence cell viability and apoptosis of NSCs and also their astrocytic differentiation. It is concluded that accumulating dose of GSM 900-MHz RF-EMF might have devastating effects on NSCs proliferation and neurogenesis requiring more causations in terms of using mobile devices. Korean Association of Anatomists 2017-06 2017-06-27 /pmc/articles/PMC5509895/ /pubmed/28713615 http://dx.doi.org/10.5115/acb.2017.50.2.115 Text en Copyright © 2017. Anatomy & Cell Biology http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Eghlidospour, Mahsa
Ghanbari, Amir
Mortazavi, Seyyed Mohammad Javad
Azari, Hassan
Effects of radiofrequency exposure emitted from a GSM mobile phone on proliferation, differentiation, and apoptosis of neural stem cells
title Effects of radiofrequency exposure emitted from a GSM mobile phone on proliferation, differentiation, and apoptosis of neural stem cells
title_full Effects of radiofrequency exposure emitted from a GSM mobile phone on proliferation, differentiation, and apoptosis of neural stem cells
title_fullStr Effects of radiofrequency exposure emitted from a GSM mobile phone on proliferation, differentiation, and apoptosis of neural stem cells
title_full_unstemmed Effects of radiofrequency exposure emitted from a GSM mobile phone on proliferation, differentiation, and apoptosis of neural stem cells
title_short Effects of radiofrequency exposure emitted from a GSM mobile phone on proliferation, differentiation, and apoptosis of neural stem cells
title_sort effects of radiofrequency exposure emitted from a gsm mobile phone on proliferation, differentiation, and apoptosis of neural stem cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509895/
https://www.ncbi.nlm.nih.gov/pubmed/28713615
http://dx.doi.org/10.5115/acb.2017.50.2.115
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