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Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes

Background: Oleoresin capsicum (OC) is an organic extract from fruits of the genus Capsicum, and has been reported to have an anti-obesity effect. Objective: This study comparatively investigated lipolytic effects of single-layer nanoemulsion (SN) and alginate double-layer nanoemulsion (AN) containi...

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Autores principales: Lee, Mak-Soon, Jung, Sunyoon, Shin, Yoonjin, Lee, Seohyun, Kim, Chong-Tai, Kim, In-Hwan, Kim, Yangha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5510203/
https://www.ncbi.nlm.nih.gov/pubmed/28747860
http://dx.doi.org/10.1080/16546628.2017.1339553
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author Lee, Mak-Soon
Jung, Sunyoon
Shin, Yoonjin
Lee, Seohyun
Kim, Chong-Tai
Kim, In-Hwan
Kim, Yangha
author_facet Lee, Mak-Soon
Jung, Sunyoon
Shin, Yoonjin
Lee, Seohyun
Kim, Chong-Tai
Kim, In-Hwan
Kim, Yangha
author_sort Lee, Mak-Soon
collection PubMed
description Background: Oleoresin capsicum (OC) is an organic extract from fruits of the genus Capsicum, and has been reported to have an anti-obesity effect. Objective: This study comparatively investigated lipolytic effects of single-layer nanoemulsion (SN) and alginate double-layer nanoemulsion (AN) containing OC in 3T3-L1 adipocytes. Methods: SN and AN were compared by analyzing the intracellular lipid accumulation, triglyceride (TG) content, release of free fatty acids (FFAs) and glycerol, and mRNA expression of genes related to adipogenesis and lipolysis were analyzed in fully differentiated 3T3-L1 adipocytes. Results: Compared with SN, AN exhibited higher efficiency in inhibiting the intracellular lipid accumulation and TG content, and enhanced the release of FFAs and glycerol into the medium. In AN-treated cells, mRNA levels of peroxisome proliferator-activated receptor-γ and the fatty acid-binding protein adipocyte protein-2, which are involved in adipogenesis, were down-regulated, whereas those of genes related to lipolysis, including hormone-sensitive lipase and carnitine palmitoyl transferase-1α, were up-regulated compared with SN-treated cells. Conclusion: The lipolytic effect of AN was greater than that of SN; this was partly associated with the increased TG hydrolysis via induction of lipolytic gene expression and suppression of adipogenic gene expression in 3T3-L1 adipocytes.​​​​
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spelling pubmed-55102032017-07-26 Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes Lee, Mak-Soon Jung, Sunyoon Shin, Yoonjin Lee, Seohyun Kim, Chong-Tai Kim, In-Hwan Kim, Yangha Food Nutr Res Original Article Background: Oleoresin capsicum (OC) is an organic extract from fruits of the genus Capsicum, and has been reported to have an anti-obesity effect. Objective: This study comparatively investigated lipolytic effects of single-layer nanoemulsion (SN) and alginate double-layer nanoemulsion (AN) containing OC in 3T3-L1 adipocytes. Methods: SN and AN were compared by analyzing the intracellular lipid accumulation, triglyceride (TG) content, release of free fatty acids (FFAs) and glycerol, and mRNA expression of genes related to adipogenesis and lipolysis were analyzed in fully differentiated 3T3-L1 adipocytes. Results: Compared with SN, AN exhibited higher efficiency in inhibiting the intracellular lipid accumulation and TG content, and enhanced the release of FFAs and glycerol into the medium. In AN-treated cells, mRNA levels of peroxisome proliferator-activated receptor-γ and the fatty acid-binding protein adipocyte protein-2, which are involved in adipogenesis, were down-regulated, whereas those of genes related to lipolysis, including hormone-sensitive lipase and carnitine palmitoyl transferase-1α, were up-regulated compared with SN-treated cells. Conclusion: The lipolytic effect of AN was greater than that of SN; this was partly associated with the increased TG hydrolysis via induction of lipolytic gene expression and suppression of adipogenic gene expression in 3T3-L1 adipocytes.​​​​ Taylor & Francis 2017-06-29 /pmc/articles/PMC5510203/ /pubmed/28747860 http://dx.doi.org/10.1080/16546628.2017.1339553 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Mak-Soon
Jung, Sunyoon
Shin, Yoonjin
Lee, Seohyun
Kim, Chong-Tai
Kim, In-Hwan
Kim, Yangha
Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes
title Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes
title_full Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes
title_fullStr Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes
title_full_unstemmed Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes
title_short Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes
title_sort lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3t3-l1 adipocytes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5510203/
https://www.ncbi.nlm.nih.gov/pubmed/28747860
http://dx.doi.org/10.1080/16546628.2017.1339553
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