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Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors
There is no available blood marker for the preoperative diagnosis of adrenocortical malignancy. The objective of this study was to investigate the expression of extracellular vesicle-associated microRNAs and their diagnostic potential in plasma samples of patients suffering from adrenocortical tumor...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5511159/ https://www.ncbi.nlm.nih.gov/pubmed/28710381 http://dx.doi.org/10.1038/s41598-017-05777-0 |
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author | Perge, Pál Butz, Henriett Pezzani, Raffaele Bancos, Irina Nagy, Zoltán Pálóczi, Krisztina Nyírő, Gábor Decmann, Ábel Pap, Erna Luconi, Michaela Mannelli, Massimo Buzás, Edit I. Tóth, Miklós Boscaro, Marco Patócs, Attila Igaz, Peter |
author_facet | Perge, Pál Butz, Henriett Pezzani, Raffaele Bancos, Irina Nagy, Zoltán Pálóczi, Krisztina Nyírő, Gábor Decmann, Ábel Pap, Erna Luconi, Michaela Mannelli, Massimo Buzás, Edit I. Tóth, Miklós Boscaro, Marco Patócs, Attila Igaz, Peter |
author_sort | Perge, Pál |
collection | PubMed |
description | There is no available blood marker for the preoperative diagnosis of adrenocortical malignancy. The objective of this study was to investigate the expression of extracellular vesicle-associated microRNAs and their diagnostic potential in plasma samples of patients suffering from adrenocortical tumors. Extracellular vesicles were isolated either by using Total Exosome Isolation Kit or by differential centrifugation/ultracentrifugation. Preoperative plasma extracellular vesicle samples of 6 adrenocortical adenomas (ACA) and 6 histologically verified adrenocortical cancer (ACC) were first screened by Taqman Human Microarray A-cards. Based on the results of screening, two miRNAs were selected and validated by targeted quantitative real-time PCR. The validation cohort included 18 ACAs and 16 ACCs. Beside RNA analysis, extracellular vesicle preparations were also assessed by transmission electron microscopy, flow cytometry and dynamic light scattering. Significant overexpression of hsa-miR-101 and hsa-miR-483-5p in ACC relative to ACA samples has been validated. Receiver operator characteristics of data revealed dCT(hsa-miR-483-5p) normalized to cel-miR-39 to have the highest diagnostic accuracy (area under curve 0.965), the sensitivity and the specifity were 87.5 and 94.44, respectively. Extracellular vesicle-associated hsa-miR-483-5p thus appears to be a promising minimally invasive biomarker in the preoperative diagnosis of ACC but needs further validation in larger cohorts of patients. |
format | Online Article Text |
id | pubmed-5511159 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55111592017-07-17 Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors Perge, Pál Butz, Henriett Pezzani, Raffaele Bancos, Irina Nagy, Zoltán Pálóczi, Krisztina Nyírő, Gábor Decmann, Ábel Pap, Erna Luconi, Michaela Mannelli, Massimo Buzás, Edit I. Tóth, Miklós Boscaro, Marco Patócs, Attila Igaz, Peter Sci Rep Article There is no available blood marker for the preoperative diagnosis of adrenocortical malignancy. The objective of this study was to investigate the expression of extracellular vesicle-associated microRNAs and their diagnostic potential in plasma samples of patients suffering from adrenocortical tumors. Extracellular vesicles were isolated either by using Total Exosome Isolation Kit or by differential centrifugation/ultracentrifugation. Preoperative plasma extracellular vesicle samples of 6 adrenocortical adenomas (ACA) and 6 histologically verified adrenocortical cancer (ACC) were first screened by Taqman Human Microarray A-cards. Based on the results of screening, two miRNAs were selected and validated by targeted quantitative real-time PCR. The validation cohort included 18 ACAs and 16 ACCs. Beside RNA analysis, extracellular vesicle preparations were also assessed by transmission electron microscopy, flow cytometry and dynamic light scattering. Significant overexpression of hsa-miR-101 and hsa-miR-483-5p in ACC relative to ACA samples has been validated. Receiver operator characteristics of data revealed dCT(hsa-miR-483-5p) normalized to cel-miR-39 to have the highest diagnostic accuracy (area under curve 0.965), the sensitivity and the specifity were 87.5 and 94.44, respectively. Extracellular vesicle-associated hsa-miR-483-5p thus appears to be a promising minimally invasive biomarker in the preoperative diagnosis of ACC but needs further validation in larger cohorts of patients. Nature Publishing Group UK 2017-07-14 /pmc/articles/PMC5511159/ /pubmed/28710381 http://dx.doi.org/10.1038/s41598-017-05777-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Perge, Pál Butz, Henriett Pezzani, Raffaele Bancos, Irina Nagy, Zoltán Pálóczi, Krisztina Nyírő, Gábor Decmann, Ábel Pap, Erna Luconi, Michaela Mannelli, Massimo Buzás, Edit I. Tóth, Miklós Boscaro, Marco Patócs, Attila Igaz, Peter Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors |
title | Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors |
title_full | Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors |
title_fullStr | Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors |
title_full_unstemmed | Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors |
title_short | Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors |
title_sort | evaluation and diagnostic potential of circulating extracellular vesicle-associated micrornas in adrenocortical tumors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5511159/ https://www.ncbi.nlm.nih.gov/pubmed/28710381 http://dx.doi.org/10.1038/s41598-017-05777-0 |
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