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Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping
Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5511349/ https://www.ncbi.nlm.nih.gov/pubmed/28703221 http://dx.doi.org/10.1038/ncomms15993 |
| _version_ | 1783250324518076416 |
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| author | Morgan, Stefanie L. Mariano, Natasha C. Bermudez, Abel Arruda, Nicole L. Wu, Fangting Luo, Yunhai Shankar, Gautam Jia, Lin Chen, Huiling Hu, Ji-Fan Hoffman, Andrew R. Huang, Chiao-Chain Pitteri, Sharon J. Wang, Kevin C. |
| author_facet | Morgan, Stefanie L. Mariano, Natasha C. Bermudez, Abel Arruda, Nicole L. Wu, Fangting Luo, Yunhai Shankar, Gautam Jia, Lin Chen, Huiling Hu, Ji-Fan Hoffman, Andrew R. Huang, Chiao-Chain Pitteri, Sharon J. Wang, Kevin C. |
| author_sort | Morgan, Stefanie L. |
| collection | PubMed |
| description | Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci. |
| format | Online Article Text |
| id | pubmed-5511349 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-55113492017-07-20 Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping Morgan, Stefanie L. Mariano, Natasha C. Bermudez, Abel Arruda, Nicole L. Wu, Fangting Luo, Yunhai Shankar, Gautam Jia, Lin Chen, Huiling Hu, Ji-Fan Hoffman, Andrew R. Huang, Chiao-Chain Pitteri, Sharon J. Wang, Kevin C. Nat Commun Article Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci. Nature Publishing Group 2017-07-13 /pmc/articles/PMC5511349/ /pubmed/28703221 http://dx.doi.org/10.1038/ncomms15993 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| spellingShingle | Article Morgan, Stefanie L. Mariano, Natasha C. Bermudez, Abel Arruda, Nicole L. Wu, Fangting Luo, Yunhai Shankar, Gautam Jia, Lin Chen, Huiling Hu, Ji-Fan Hoffman, Andrew R. Huang, Chiao-Chain Pitteri, Sharon J. Wang, Kevin C. Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping |
| title | Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping |
| title_full | Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping |
| title_fullStr | Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping |
| title_full_unstemmed | Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping |
| title_short | Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping |
| title_sort | manipulation of nuclear architecture through crispr-mediated chromosomal looping |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5511349/ https://www.ncbi.nlm.nih.gov/pubmed/28703221 http://dx.doi.org/10.1038/ncomms15993 |
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