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Antiplasmodial activity and cytotoxicity of ethanol extract of Zea mays root
OBJECTIVE: Zea mays root decoction that has been traditionally used for the treatment of malaria by various tribes in Nigeria, was evaluated for antimalarial potential against malaria parasites using in vivo and in vitro models. MATERIALS AND METHODS: The root extract of Zea mays was investigated fo...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5511979/ https://www.ncbi.nlm.nih.gov/pubmed/28748174 |
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author | Okokon, Jude Efiom Antia, Bassey Sunday Azare, Bala Adamu Okokon, Patience Jude |
author_facet | Okokon, Jude Efiom Antia, Bassey Sunday Azare, Bala Adamu Okokon, Patience Jude |
author_sort | Okokon, Jude Efiom |
collection | PubMed |
description | OBJECTIVE: Zea mays root decoction that has been traditionally used for the treatment of malaria by various tribes in Nigeria, was evaluated for antimalarial potential against malaria parasites using in vivo and in vitro models. MATERIALS AND METHODS: The root extract of Zea mays was investigated for antimalarial activity against Plasmodium berghei in mice using rodent malaria models; suppressive, prophylactic and curative tests and in vitro antiplasmodial activity against chloroquine-sensitive (Pf 3D7) and resistant (Pf INDO) strains of Plasmodium falciparum using SYBR green assay method. Median lethal dose and cytotoxic activity against HeLa and HEKS cells were assessed and phytochemical screening was also carried out using standard procedures. RESULTS: The LD(50) value of root extract was found to be 474.34 mg/kg. The crude extract (45-135 mg/kg, p.o) showed significant (p<0.05-0.001) antimalarial activity against P. berghei infection in suppressive, prophylactic and curative tests with a prolonged survival time. The crude extract also showed moderate activity against both chloroquine-sensitive (Pf 3D7) and resistant (Pf INDO) strains of P. falciparum with an IC(50) value of 71.62±3.38 μg/ml (for Pf 3D7) and 63.76±4.12 μg/ml (for Pf INDO). The crude extract was not cytotoxic to the two cell lines tested with TC(50) of >100 μg/ml against both HeLa and HEKS cell lines. CONCLUSION: These results suggest that the root extract of Zea mays possesses antimalarial activity against both chloroquine-sensitive and resistant malaria and these data justify its use in ethnomedicine to treat malaria infections. |
format | Online Article Text |
id | pubmed-5511979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-55119792017-07-26 Antiplasmodial activity and cytotoxicity of ethanol extract of Zea mays root Okokon, Jude Efiom Antia, Bassey Sunday Azare, Bala Adamu Okokon, Patience Jude Avicenna J Phytomed Original Article OBJECTIVE: Zea mays root decoction that has been traditionally used for the treatment of malaria by various tribes in Nigeria, was evaluated for antimalarial potential against malaria parasites using in vivo and in vitro models. MATERIALS AND METHODS: The root extract of Zea mays was investigated for antimalarial activity against Plasmodium berghei in mice using rodent malaria models; suppressive, prophylactic and curative tests and in vitro antiplasmodial activity against chloroquine-sensitive (Pf 3D7) and resistant (Pf INDO) strains of Plasmodium falciparum using SYBR green assay method. Median lethal dose and cytotoxic activity against HeLa and HEKS cells were assessed and phytochemical screening was also carried out using standard procedures. RESULTS: The LD(50) value of root extract was found to be 474.34 mg/kg. The crude extract (45-135 mg/kg, p.o) showed significant (p<0.05-0.001) antimalarial activity against P. berghei infection in suppressive, prophylactic and curative tests with a prolonged survival time. The crude extract also showed moderate activity against both chloroquine-sensitive (Pf 3D7) and resistant (Pf INDO) strains of P. falciparum with an IC(50) value of 71.62±3.38 μg/ml (for Pf 3D7) and 63.76±4.12 μg/ml (for Pf INDO). The crude extract was not cytotoxic to the two cell lines tested with TC(50) of >100 μg/ml against both HeLa and HEKS cell lines. CONCLUSION: These results suggest that the root extract of Zea mays possesses antimalarial activity against both chloroquine-sensitive and resistant malaria and these data justify its use in ethnomedicine to treat malaria infections. Mashhad University of Medical Sciences 2017 /pmc/articles/PMC5511979/ /pubmed/28748174 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Okokon, Jude Efiom Antia, Bassey Sunday Azare, Bala Adamu Okokon, Patience Jude Antiplasmodial activity and cytotoxicity of ethanol extract of Zea mays root |
title | Antiplasmodial activity and cytotoxicity of ethanol extract of Zea mays root |
title_full | Antiplasmodial activity and cytotoxicity of ethanol extract of Zea mays root |
title_fullStr | Antiplasmodial activity and cytotoxicity of ethanol extract of Zea mays root |
title_full_unstemmed | Antiplasmodial activity and cytotoxicity of ethanol extract of Zea mays root |
title_short | Antiplasmodial activity and cytotoxicity of ethanol extract of Zea mays root |
title_sort | antiplasmodial activity and cytotoxicity of ethanol extract of zea mays root |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5511979/ https://www.ncbi.nlm.nih.gov/pubmed/28748174 |
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