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Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro
The objective of this study was to assess the effects of exogenously expressed proinsulin on the biological characters of a hematopoietic stem cell line (HSC) and erythroid myeloid lymphoid (EML) cells and explore new strategies for cell therapy for type I diabetes. EML cells were transduced with le...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5512049/ https://www.ncbi.nlm.nih.gov/pubmed/28758130 http://dx.doi.org/10.1155/2017/5649191 |
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author | Han, Yuewen Liu, Tingting Zou, Yunding Ji, Ling Li, Yuanyuan Li, Jing Wang, Jing Chen, Guopin Chen, Jieping Chen, Liang Ye, Zhijia |
author_facet | Han, Yuewen Liu, Tingting Zou, Yunding Ji, Ling Li, Yuanyuan Li, Jing Wang, Jing Chen, Guopin Chen, Jieping Chen, Liang Ye, Zhijia |
author_sort | Han, Yuewen |
collection | PubMed |
description | The objective of this study was to assess the effects of exogenously expressed proinsulin on the biological characters of a hematopoietic stem cell line (HSC) and erythroid myeloid lymphoid (EML) cells and explore new strategies for cell therapy for type I diabetes. EML cells were transduced with lentivirus particles carrying the human proinsulin (proINS) gene. The positive transduced cells were selected based on green fluorescence protein (GFP) positivity and puromycin resistance. Overexpression of proINS was confirmed via real-time PCR and Western blotting. The functional activity of the human proINS secreted by EML cells was elucidated by analyzing the activation of insulin receptor and its downstream signaling. Pro-INS + EML cells were able to prime the phosphorylation of insulin receptor as well as induce the expression of downstream genes of insulin receptor. Furthermore, Wnt3a can significantly promote self-renewal of Pro-INS + EML cells. However, we did not observe significant changes in the proliferation and differentiation of INS + EML cells, compared to the control EML cells. Our results might be useful for developing a new therapy for diabetes mellitus. |
format | Online Article Text |
id | pubmed-5512049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-55120492017-07-30 Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro Han, Yuewen Liu, Tingting Zou, Yunding Ji, Ling Li, Yuanyuan Li, Jing Wang, Jing Chen, Guopin Chen, Jieping Chen, Liang Ye, Zhijia J Diabetes Res Research Article The objective of this study was to assess the effects of exogenously expressed proinsulin on the biological characters of a hematopoietic stem cell line (HSC) and erythroid myeloid lymphoid (EML) cells and explore new strategies for cell therapy for type I diabetes. EML cells were transduced with lentivirus particles carrying the human proinsulin (proINS) gene. The positive transduced cells were selected based on green fluorescence protein (GFP) positivity and puromycin resistance. Overexpression of proINS was confirmed via real-time PCR and Western blotting. The functional activity of the human proINS secreted by EML cells was elucidated by analyzing the activation of insulin receptor and its downstream signaling. Pro-INS + EML cells were able to prime the phosphorylation of insulin receptor as well as induce the expression of downstream genes of insulin receptor. Furthermore, Wnt3a can significantly promote self-renewal of Pro-INS + EML cells. However, we did not observe significant changes in the proliferation and differentiation of INS + EML cells, compared to the control EML cells. Our results might be useful for developing a new therapy for diabetes mellitus. Hindawi 2017 2017-07-03 /pmc/articles/PMC5512049/ /pubmed/28758130 http://dx.doi.org/10.1155/2017/5649191 Text en Copyright © 2017 Yuewen Han et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Han, Yuewen Liu, Tingting Zou, Yunding Ji, Ling Li, Yuanyuan Li, Jing Wang, Jing Chen, Guopin Chen, Jieping Chen, Liang Ye, Zhijia Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro |
title | Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro |
title_full | Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro |
title_fullStr | Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro |
title_full_unstemmed | Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro |
title_short | Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro |
title_sort | proinsulin promotes self-renewal of a hematopoietic progenitor cell line in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5512049/ https://www.ncbi.nlm.nih.gov/pubmed/28758130 http://dx.doi.org/10.1155/2017/5649191 |
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