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Draft sequencing and assembly of the genome of the world’s largest fish, the whale shark: Rhincodon typus Smith 1828

BACKGROUND: The whale shark (Rhincodon typus) has by far the largest body size of any elasmobranch (shark or ray) species. Therefore, it is also the largest extant species of the paraphyletic assemblage commonly referred to as fishes. As both a phenotypic extreme and a member of the group Chondricht...

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Autores principales: Read, Timothy D., Petit, Robert A., Joseph, Sandeep J., Alam, Md. Tauqeer, Weil, M. Ryan, Ahmad, Maida, Bhimani, Ravila, Vuong, Jocelyn S., Haase, Chad P., Webb, D. Harry, Tan, Milton, Dove, Alistair D. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5513125/
https://www.ncbi.nlm.nih.gov/pubmed/28709399
http://dx.doi.org/10.1186/s12864-017-3926-9
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author Read, Timothy D.
Petit, Robert A.
Joseph, Sandeep J.
Alam, Md. Tauqeer
Weil, M. Ryan
Ahmad, Maida
Bhimani, Ravila
Vuong, Jocelyn S.
Haase, Chad P.
Webb, D. Harry
Tan, Milton
Dove, Alistair D. M.
author_facet Read, Timothy D.
Petit, Robert A.
Joseph, Sandeep J.
Alam, Md. Tauqeer
Weil, M. Ryan
Ahmad, Maida
Bhimani, Ravila
Vuong, Jocelyn S.
Haase, Chad P.
Webb, D. Harry
Tan, Milton
Dove, Alistair D. M.
author_sort Read, Timothy D.
collection PubMed
description BACKGROUND: The whale shark (Rhincodon typus) has by far the largest body size of any elasmobranch (shark or ray) species. Therefore, it is also the largest extant species of the paraphyletic assemblage commonly referred to as fishes. As both a phenotypic extreme and a member of the group Chondrichthyes – the sister group to the remaining gnathostomes, which includes all tetrapods and therefore also humans – its genome is of substantial comparative interest. Whale sharks are also listed as an endangered species on the International Union for Conservation of Nature’s Red List of threatened species and are of growing popularity as both a target of ecotourism and as a charismatic conservation ambassador for the pelagic ecosystem. A genome map for this species would aid in defining effective conservation units and understanding global population structure. RESULTS: We characterised the nuclear genome of the whale shark using next generation sequencing (454, Illumina) and de novo assembly and annotation methods, based on material collected from the Georgia Aquarium. The data set consisted of 878,654,233 reads, which yielded a draft assembly of 1,213,200 contigs and 997,976 scaffolds. The estimated genome size was 3.44Gb. As expected, the proteome of the whale shark was most closely related to the only other complete genome of a cartilaginous fish, the holocephalan elephant shark. The whale shark contained a novel Toll-like-receptor (TLR) protein with sequence similarity to both the TLR4 and TLR13 proteins of mammals and TLR21 of teleosts. The data are publicly available on GenBank, FigShare, and from the NCBI Short Read Archive under accession number SRP044374. CONCLUSIONS: This represents the first shotgun elasmobranch genome and will aid studies of molecular systematics, biogeography, genetic differentiation, and conservation genetics in this and other shark species, as well as providing comparative data for studies of evolutionary biology and immunology across the jawed vertebrate lineages.
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spelling pubmed-55131252017-07-19 Draft sequencing and assembly of the genome of the world’s largest fish, the whale shark: Rhincodon typus Smith 1828 Read, Timothy D. Petit, Robert A. Joseph, Sandeep J. Alam, Md. Tauqeer Weil, M. Ryan Ahmad, Maida Bhimani, Ravila Vuong, Jocelyn S. Haase, Chad P. Webb, D. Harry Tan, Milton Dove, Alistair D. M. BMC Genomics Research Article BACKGROUND: The whale shark (Rhincodon typus) has by far the largest body size of any elasmobranch (shark or ray) species. Therefore, it is also the largest extant species of the paraphyletic assemblage commonly referred to as fishes. As both a phenotypic extreme and a member of the group Chondrichthyes – the sister group to the remaining gnathostomes, which includes all tetrapods and therefore also humans – its genome is of substantial comparative interest. Whale sharks are also listed as an endangered species on the International Union for Conservation of Nature’s Red List of threatened species and are of growing popularity as both a target of ecotourism and as a charismatic conservation ambassador for the pelagic ecosystem. A genome map for this species would aid in defining effective conservation units and understanding global population structure. RESULTS: We characterised the nuclear genome of the whale shark using next generation sequencing (454, Illumina) and de novo assembly and annotation methods, based on material collected from the Georgia Aquarium. The data set consisted of 878,654,233 reads, which yielded a draft assembly of 1,213,200 contigs and 997,976 scaffolds. The estimated genome size was 3.44Gb. As expected, the proteome of the whale shark was most closely related to the only other complete genome of a cartilaginous fish, the holocephalan elephant shark. The whale shark contained a novel Toll-like-receptor (TLR) protein with sequence similarity to both the TLR4 and TLR13 proteins of mammals and TLR21 of teleosts. The data are publicly available on GenBank, FigShare, and from the NCBI Short Read Archive under accession number SRP044374. CONCLUSIONS: This represents the first shotgun elasmobranch genome and will aid studies of molecular systematics, biogeography, genetic differentiation, and conservation genetics in this and other shark species, as well as providing comparative data for studies of evolutionary biology and immunology across the jawed vertebrate lineages. BioMed Central 2017-07-14 /pmc/articles/PMC5513125/ /pubmed/28709399 http://dx.doi.org/10.1186/s12864-017-3926-9 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Read, Timothy D.
Petit, Robert A.
Joseph, Sandeep J.
Alam, Md. Tauqeer
Weil, M. Ryan
Ahmad, Maida
Bhimani, Ravila
Vuong, Jocelyn S.
Haase, Chad P.
Webb, D. Harry
Tan, Milton
Dove, Alistair D. M.
Draft sequencing and assembly of the genome of the world’s largest fish, the whale shark: Rhincodon typus Smith 1828
title Draft sequencing and assembly of the genome of the world’s largest fish, the whale shark: Rhincodon typus Smith 1828
title_full Draft sequencing and assembly of the genome of the world’s largest fish, the whale shark: Rhincodon typus Smith 1828
title_fullStr Draft sequencing and assembly of the genome of the world’s largest fish, the whale shark: Rhincodon typus Smith 1828
title_full_unstemmed Draft sequencing and assembly of the genome of the world’s largest fish, the whale shark: Rhincodon typus Smith 1828
title_short Draft sequencing and assembly of the genome of the world’s largest fish, the whale shark: Rhincodon typus Smith 1828
title_sort draft sequencing and assembly of the genome of the world’s largest fish, the whale shark: rhincodon typus smith 1828
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5513125/
https://www.ncbi.nlm.nih.gov/pubmed/28709399
http://dx.doi.org/10.1186/s12864-017-3926-9
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