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Cofactor engineering improved CALB production in Pichia pastoris through heterologous expression of NADH oxidase and adenylate kinase

The cofactor engineering strategy can relieve the metabolic stress induced by expression of recombinant protein in cellular metabolism related to cofactor and energy reactions. To study the effect of cofactor regeneration on recombinant protein expression, NADH oxidase (noxE) was engineered in P. pa...

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Detalles Bibliográficos
Autores principales:	Jayachandran, Charumathi, Palanisamy Athiyaman, Balakumaran, Sankaranarayanan, Meenakshisundaram
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5513558/ https://www.ncbi.nlm.nih.gov/pubmed/28715469 http://dx.doi.org/10.1371/journal.pone.0181370

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