Morphological, Immunocytochemical, and Biochemical Studies of Rat Costal Chondrocytes Exposed to IL-1β and TGF-β1

This study was undertaken to determine the effects of IL-1β and TGF-β1 on the expression of differentiation-associated genes in chondrocytes in vitro. Rat costal chondrocytes were exposed to different concentrations of IL-1β and TGF-β1 for 48 h and tested for gene expression. IL-1β increased the exp...

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Detalles Bibliográficos
Autores principales: Li, Xiaoli, Ren, Xiaoyong, Li, Sisi, Liang, Jianmin, Zhao, Xiaoyan, Wang, Ting, Wang, Zhenghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5514342/
https://www.ncbi.nlm.nih.gov/pubmed/29065675
http://dx.doi.org/10.1155/2017/9747264
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author Li, Xiaoli
Ren, Xiaoyong
Li, Sisi
Liang, Jianmin
Zhao, Xiaoyan
Wang, Ting
Wang, Zhenghui
author_facet Li, Xiaoli
Ren, Xiaoyong
Li, Sisi
Liang, Jianmin
Zhao, Xiaoyan
Wang, Ting
Wang, Zhenghui
author_sort Li, Xiaoli
collection PubMed
description This study was undertaken to determine the effects of IL-1β and TGF-β1 on the expression of differentiation-associated genes in chondrocytes in vitro. Rat costal chondrocytes were exposed to different concentrations of IL-1β and TGF-β1 for 48 h and tested for gene expression. IL-1β increased the expression of aggrecanase-1 and aggrecanase-2 and decreased the content of aggrecan and collagen II. Low concentration of TGF-β1 decreased the expression of aggrecan and collagen II and increased the expression of aggrecanase-2. However, the level of aggrecanase-1 was significantly elevated in the presence of high concentration of TGF-β1. IL-1β and TGF-β1 show the ability to modulate the production of aggrecan and collagen II in chondrocytes in vitro.
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spelling pubmed-55143422017-07-25 Morphological, Immunocytochemical, and Biochemical Studies of Rat Costal Chondrocytes Exposed to IL-1β and TGF-β1 Li, Xiaoli Ren, Xiaoyong Li, Sisi Liang, Jianmin Zhao, Xiaoyan Wang, Ting Wang, Zhenghui J Healthc Eng Research Article This study was undertaken to determine the effects of IL-1β and TGF-β1 on the expression of differentiation-associated genes in chondrocytes in vitro. Rat costal chondrocytes were exposed to different concentrations of IL-1β and TGF-β1 for 48 h and tested for gene expression. IL-1β increased the expression of aggrecanase-1 and aggrecanase-2 and decreased the content of aggrecan and collagen II. Low concentration of TGF-β1 decreased the expression of aggrecan and collagen II and increased the expression of aggrecanase-2. However, the level of aggrecanase-1 was significantly elevated in the presence of high concentration of TGF-β1. IL-1β and TGF-β1 show the ability to modulate the production of aggrecan and collagen II in chondrocytes in vitro. Hindawi 2017 2017-07-04 /pmc/articles/PMC5514342/ /pubmed/29065675 http://dx.doi.org/10.1155/2017/9747264 Text en Copyright © 2017 Xiaoli Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Xiaoli
Ren, Xiaoyong
Li, Sisi
Liang, Jianmin
Zhao, Xiaoyan
Wang, Ting
Wang, Zhenghui
Morphological, Immunocytochemical, and Biochemical Studies of Rat Costal Chondrocytes Exposed to IL-1β and TGF-β1
title Morphological, Immunocytochemical, and Biochemical Studies of Rat Costal Chondrocytes Exposed to IL-1β and TGF-β1
title_full Morphological, Immunocytochemical, and Biochemical Studies of Rat Costal Chondrocytes Exposed to IL-1β and TGF-β1
title_fullStr Morphological, Immunocytochemical, and Biochemical Studies of Rat Costal Chondrocytes Exposed to IL-1β and TGF-β1
title_full_unstemmed Morphological, Immunocytochemical, and Biochemical Studies of Rat Costal Chondrocytes Exposed to IL-1β and TGF-β1
title_short Morphological, Immunocytochemical, and Biochemical Studies of Rat Costal Chondrocytes Exposed to IL-1β and TGF-β1
title_sort morphological, immunocytochemical, and biochemical studies of rat costal chondrocytes exposed to il-1β and tgf-β1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5514342/
https://www.ncbi.nlm.nih.gov/pubmed/29065675
http://dx.doi.org/10.1155/2017/9747264
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