Cargando…
Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis
This study aimed to investigate the antifertility effect of Juniperus sabina fruit on male rats and its possible mechanism, and hence it might be developed as a potential nonhormonal male contraceptive. Male rats were intragastrically fed for consecutive 8-week and 4-week recovery with the fruit of...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5514346/ https://www.ncbi.nlm.nih.gov/pubmed/28744317 http://dx.doi.org/10.1155/2017/6958982 |
_version_ | 1783250827275665408 |
---|---|
author | Xie, Shuwu Li, Guoting Qu, Lijuan Zhong, Ruihua Chen, Ping Lu, Zhigang Zhou, Jieyun Guo, Xiangjie Li, Zhao Ma, Aying Qian, Yueying Zhu, Yan |
author_facet | Xie, Shuwu Li, Guoting Qu, Lijuan Zhong, Ruihua Chen, Ping Lu, Zhigang Zhou, Jieyun Guo, Xiangjie Li, Zhao Ma, Aying Qian, Yueying Zhu, Yan |
author_sort | Xie, Shuwu |
collection | PubMed |
description | This study aimed to investigate the antifertility effect of Juniperus sabina fruit on male rats and its possible mechanism, and hence it might be developed as a potential nonhormonal male contraceptive. Male rats were intragastrically fed for consecutive 8-week and 4-week recovery with the fruit of J. Sabina, and sperm maturation, serum testosterone level, and histopathology were analyzed. Epididymal epithelial cell culture was prepared for detection of podophyllotoxin activities. Furthermore, cell proliferation, transmission electron microscopy, Annexin V/Propidium iodide, TUNEL, RT-PCR, ELISA, and western blotting were examined. The results showed that rat sperm motility and fertility were remarkably declined after feeding the fruit. Moreover, the fruit targeted the epididymis rather than the testis. After 4-week recovery, more than half of the male rats resumed normal fertility. It was found that podophyllotoxin significantly inhibited epididymal epithelial cell proliferation, promoted cell apoptosis, and increased the mRNA and protein levels of TNF-α and the expression levels of cytochrome c, caspase-8, caspase-9, and caspase-3. Our findings suggest that the fruit of J. sabina could inhibit male rat sperm maturation and fertility. The potential mechanism might be related to podophyllotoxin, inducing epididymal epithelial cell apoptosis through TNF-α and caspase signaling pathway. |
format | Online Article Text |
id | pubmed-5514346 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-55143462017-07-25 Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis Xie, Shuwu Li, Guoting Qu, Lijuan Zhong, Ruihua Chen, Ping Lu, Zhigang Zhou, Jieyun Guo, Xiangjie Li, Zhao Ma, Aying Qian, Yueying Zhu, Yan Evid Based Complement Alternat Med Research Article This study aimed to investigate the antifertility effect of Juniperus sabina fruit on male rats and its possible mechanism, and hence it might be developed as a potential nonhormonal male contraceptive. Male rats were intragastrically fed for consecutive 8-week and 4-week recovery with the fruit of J. Sabina, and sperm maturation, serum testosterone level, and histopathology were analyzed. Epididymal epithelial cell culture was prepared for detection of podophyllotoxin activities. Furthermore, cell proliferation, transmission electron microscopy, Annexin V/Propidium iodide, TUNEL, RT-PCR, ELISA, and western blotting were examined. The results showed that rat sperm motility and fertility were remarkably declined after feeding the fruit. Moreover, the fruit targeted the epididymis rather than the testis. After 4-week recovery, more than half of the male rats resumed normal fertility. It was found that podophyllotoxin significantly inhibited epididymal epithelial cell proliferation, promoted cell apoptosis, and increased the mRNA and protein levels of TNF-α and the expression levels of cytochrome c, caspase-8, caspase-9, and caspase-3. Our findings suggest that the fruit of J. sabina could inhibit male rat sperm maturation and fertility. The potential mechanism might be related to podophyllotoxin, inducing epididymal epithelial cell apoptosis through TNF-α and caspase signaling pathway. Hindawi 2017 2017-07-04 /pmc/articles/PMC5514346/ /pubmed/28744317 http://dx.doi.org/10.1155/2017/6958982 Text en Copyright © 2017 Shuwu Xie et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xie, Shuwu Li, Guoting Qu, Lijuan Zhong, Ruihua Chen, Ping Lu, Zhigang Zhou, Jieyun Guo, Xiangjie Li, Zhao Ma, Aying Qian, Yueying Zhu, Yan Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis |
title | Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis |
title_full | Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis |
title_fullStr | Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis |
title_full_unstemmed | Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis |
title_short | Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis |
title_sort | podophyllotoxin extracted from juniperus sabina fruit inhibits rat sperm maturation and fertility by promoting epididymal epithelial cell apoptosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5514346/ https://www.ncbi.nlm.nih.gov/pubmed/28744317 http://dx.doi.org/10.1155/2017/6958982 |
work_keys_str_mv | AT xieshuwu podophyllotoxinextractedfromjuniperussabinafruitinhibitsratspermmaturationandfertilitybypromotingepididymalepithelialcellapoptosis AT liguoting podophyllotoxinextractedfromjuniperussabinafruitinhibitsratspermmaturationandfertilitybypromotingepididymalepithelialcellapoptosis AT qulijuan podophyllotoxinextractedfromjuniperussabinafruitinhibitsratspermmaturationandfertilitybypromotingepididymalepithelialcellapoptosis AT zhongruihua podophyllotoxinextractedfromjuniperussabinafruitinhibitsratspermmaturationandfertilitybypromotingepididymalepithelialcellapoptosis AT chenping podophyllotoxinextractedfromjuniperussabinafruitinhibitsratspermmaturationandfertilitybypromotingepididymalepithelialcellapoptosis AT luzhigang podophyllotoxinextractedfromjuniperussabinafruitinhibitsratspermmaturationandfertilitybypromotingepididymalepithelialcellapoptosis AT zhoujieyun podophyllotoxinextractedfromjuniperussabinafruitinhibitsratspermmaturationandfertilitybypromotingepididymalepithelialcellapoptosis AT guoxiangjie podophyllotoxinextractedfromjuniperussabinafruitinhibitsratspermmaturationandfertilitybypromotingepididymalepithelialcellapoptosis AT lizhao podophyllotoxinextractedfromjuniperussabinafruitinhibitsratspermmaturationandfertilitybypromotingepididymalepithelialcellapoptosis AT maaying podophyllotoxinextractedfromjuniperussabinafruitinhibitsratspermmaturationandfertilitybypromotingepididymalepithelialcellapoptosis AT qianyueying podophyllotoxinextractedfromjuniperussabinafruitinhibitsratspermmaturationandfertilitybypromotingepididymalepithelialcellapoptosis AT zhuyan podophyllotoxinextractedfromjuniperussabinafruitinhibitsratspermmaturationandfertilitybypromotingepididymalepithelialcellapoptosis |