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A minimalist fluorescent probe for differentiating Cys, Hcy and GSH in live cells
A simple molecule, tetrafluoroterephthalonitrile (4F-2CN), was discovered to be an efficient fluorescent probe for detecting biological thiol species. The probe responded to Cys and emitted strong green fluorescence, whereas it reacted with Hcy/GSH and generated blue fluorescence. Addition of CTAB (...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515046/ https://www.ncbi.nlm.nih.gov/pubmed/28758002 http://dx.doi.org/10.1039/c5sc02431e |
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author | Zhang, Huatang Liu, Ruochuan Liu, Jie Li, Lin Wang, Ping Yao, Shao Q. Xu, Zhengtao Sun, Hongyan |
author_facet | Zhang, Huatang Liu, Ruochuan Liu, Jie Li, Lin Wang, Ping Yao, Shao Q. Xu, Zhengtao Sun, Hongyan |
author_sort | Zhang, Huatang |
collection | PubMed |
description | A simple molecule, tetrafluoroterephthalonitrile (4F-2CN), was discovered to be an efficient fluorescent probe for detecting biological thiol species. The probe responded to Cys and emitted strong green fluorescence, whereas it reacted with Hcy/GSH and generated blue fluorescence. Addition of CTAB (cetyl trimethylammonium bromide) was observed to alter the fluorescence color of the reaction product of 4F-2CN and Hcy (from blue to green), but no alteration of the fluorescence color occurred for Cys and GSH. For the very first time, cell imaging experiments showed that the three commonly occurring thiols (Cys/Hcy/GSH) could be differentiated using a single fluorescent probe. In addition, the reaction product of 4F-2CN and Cys exhibits two-photon properties, offering a potentially useful tool for tissue imaging studies. To the best of our knowledge, 4F-2CN is currently the smallest fluorescent probe for thiol detection. We envision that this new and versatile probe will be a useful tool for further elucidating the roles of thiols in biology. |
format | Online Article Text |
id | pubmed-5515046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-55150462017-07-28 A minimalist fluorescent probe for differentiating Cys, Hcy and GSH in live cells Zhang, Huatang Liu, Ruochuan Liu, Jie Li, Lin Wang, Ping Yao, Shao Q. Xu, Zhengtao Sun, Hongyan Chem Sci Chemistry A simple molecule, tetrafluoroterephthalonitrile (4F-2CN), was discovered to be an efficient fluorescent probe for detecting biological thiol species. The probe responded to Cys and emitted strong green fluorescence, whereas it reacted with Hcy/GSH and generated blue fluorescence. Addition of CTAB (cetyl trimethylammonium bromide) was observed to alter the fluorescence color of the reaction product of 4F-2CN and Hcy (from blue to green), but no alteration of the fluorescence color occurred for Cys and GSH. For the very first time, cell imaging experiments showed that the three commonly occurring thiols (Cys/Hcy/GSH) could be differentiated using a single fluorescent probe. In addition, the reaction product of 4F-2CN and Cys exhibits two-photon properties, offering a potentially useful tool for tissue imaging studies. To the best of our knowledge, 4F-2CN is currently the smallest fluorescent probe for thiol detection. We envision that this new and versatile probe will be a useful tool for further elucidating the roles of thiols in biology. Royal Society of Chemistry 2016-01-01 2015-09-22 /pmc/articles/PMC5515046/ /pubmed/28758002 http://dx.doi.org/10.1039/c5sc02431e Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry Zhang, Huatang Liu, Ruochuan Liu, Jie Li, Lin Wang, Ping Yao, Shao Q. Xu, Zhengtao Sun, Hongyan A minimalist fluorescent probe for differentiating Cys, Hcy and GSH in live cells |
title | A minimalist fluorescent probe for differentiating Cys, Hcy and GSH in live cells
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title_full | A minimalist fluorescent probe for differentiating Cys, Hcy and GSH in live cells
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title_fullStr | A minimalist fluorescent probe for differentiating Cys, Hcy and GSH in live cells
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title_full_unstemmed | A minimalist fluorescent probe for differentiating Cys, Hcy and GSH in live cells
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title_short | A minimalist fluorescent probe for differentiating Cys, Hcy and GSH in live cells
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title_sort | minimalist fluorescent probe for differentiating cys, hcy and gsh in live cells |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515046/ https://www.ncbi.nlm.nih.gov/pubmed/28758002 http://dx.doi.org/10.1039/c5sc02431e |
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