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A perspective on 16S rRNA operational taxonomic unit clustering using sequence similarity

The standard pipeline for 16S amplicon analysis starts by clustering sequences within a percent sequence similarity threshold (typically 97%) into ‘Operational Taxonomic Units’ (OTUs). From each OTU, a single sequence is selected as a representative. This representative sequence is annotated, and th...

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Detalles Bibliográficos
Autores principales: Nguyen, Nam-Phuong, Warnow, Tandy, Pop, Mihai, White, Bryan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515256/
https://www.ncbi.nlm.nih.gov/pubmed/28721243
http://dx.doi.org/10.1038/npjbiofilms.2016.4