Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails

It is not always easy to apply microarray technology to small numbers of cells because of the difficulty in selectively isolating mRNA from such cells. We report here the preparation of mRNA from ciliated sensory neurons of Caenorhabditis elegans using the mRNA-tagging method, in which poly(A) RNA w...

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Detalles Bibliográficos
Autores principales: Kunitomo, Hirofumi, Uesugi, Hiroko, Kohara, Yuji, Iino, Yuichi
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551537/
https://www.ncbi.nlm.nih.gov/pubmed/15693946
http://dx.doi.org/10.1186/gb-2005-6-2-r17
Descripción
Sumario:It is not always easy to apply microarray technology to small numbers of cells because of the difficulty in selectively isolating mRNA from such cells. We report here the preparation of mRNA from ciliated sensory neurons of Caenorhabditis elegans using the mRNA-tagging method, in which poly(A) RNA was co-immunoprecipitated with an epitope-tagged poly(A)-binding protein specifically expressed in sensory neurons. Subsequent cDNA microarray analyses led to the identification of a panel of sensory neuron-expressed genes.

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