Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails

It is not always easy to apply microarray technology to small numbers of cells because of the difficulty in selectively isolating mRNA from such cells. We report here the preparation of mRNA from ciliated sensory neurons of Caenorhabditis elegans using the mRNA-tagging method, in which poly(A) RNA w...

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Detalles Bibliográficos
Autores principales: Kunitomo, Hirofumi, Uesugi, Hiroko, Kohara, Yuji, Iino, Yuichi
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551537/
https://www.ncbi.nlm.nih.gov/pubmed/15693946
http://dx.doi.org/10.1186/gb-2005-6-2-r17
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author Kunitomo, Hirofumi
Uesugi, Hiroko
Kohara, Yuji
Iino, Yuichi
author_facet Kunitomo, Hirofumi
Uesugi, Hiroko
Kohara, Yuji
Iino, Yuichi
author_sort Kunitomo, Hirofumi
collection PubMed
description It is not always easy to apply microarray technology to small numbers of cells because of the difficulty in selectively isolating mRNA from such cells. We report here the preparation of mRNA from ciliated sensory neurons of Caenorhabditis elegans using the mRNA-tagging method, in which poly(A) RNA was co-immunoprecipitated with an epitope-tagged poly(A)-binding protein specifically expressed in sensory neurons. Subsequent cDNA microarray analyses led to the identification of a panel of sensory neuron-expressed genes.
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spelling pubmed-5515372005-03-03 Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails Kunitomo, Hirofumi Uesugi, Hiroko Kohara, Yuji Iino, Yuichi Genome Biol Method It is not always easy to apply microarray technology to small numbers of cells because of the difficulty in selectively isolating mRNA from such cells. We report here the preparation of mRNA from ciliated sensory neurons of Caenorhabditis elegans using the mRNA-tagging method, in which poly(A) RNA was co-immunoprecipitated with an epitope-tagged poly(A)-binding protein specifically expressed in sensory neurons. Subsequent cDNA microarray analyses led to the identification of a panel of sensory neuron-expressed genes. BioMed Central 2005 2005-01-31 /pmc/articles/PMC551537/ /pubmed/15693946 http://dx.doi.org/10.1186/gb-2005-6-2-r17 Text en Copyright © 2005 Kunitomo et al.; licensee BioMed Central Ltd.
spellingShingle Method
Kunitomo, Hirofumi
Uesugi, Hiroko
Kohara, Yuji
Iino, Yuichi
Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails
title Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails
title_full Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails
title_fullStr Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails
title_full_unstemmed Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails
title_short Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails
title_sort identification of ciliated sensory neuron-expressed genes in caenorhabditis elegans using targeted pull-down of poly(a) tails
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551537/
https://www.ncbi.nlm.nih.gov/pubmed/15693946
http://dx.doi.org/10.1186/gb-2005-6-2-r17
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