A universal method for automated gene mapping

Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes st...

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Detalles Bibliográficos
Autores principales: Zipperlen, Peder, Nairz, Knud, Rimann, Ivo, Basler, Konrad, Hafen, Ernst, Hengartner, Michael, Hajnal, Alex
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551539/
https://www.ncbi.nlm.nih.gov/pubmed/15693948
http://dx.doi.org/10.1186/gb-2005-6-2-r19
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author Zipperlen, Peder
Nairz, Knud
Rimann, Ivo
Basler, Konrad
Hafen, Ernst
Hengartner, Michael
Hajnal, Alex
author_facet Zipperlen, Peder
Nairz, Knud
Rimann, Ivo
Basler, Konrad
Hafen, Ernst
Hengartner, Michael
Hajnal, Alex
author_sort Zipperlen, Peder
collection PubMed
description Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual input and at comparatively low cost.
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spelling pubmed-5515392005-03-03 A universal method for automated gene mapping Zipperlen, Peder Nairz, Knud Rimann, Ivo Basler, Konrad Hafen, Ernst Hengartner, Michael Hajnal, Alex Genome Biol Method Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual input and at comparatively low cost. BioMed Central 2005 2005-01-17 /pmc/articles/PMC551539/ /pubmed/15693948 http://dx.doi.org/10.1186/gb-2005-6-2-r19 Text en Copyright © 2005 Zipperlen et al.; licensee BioMed Central Ltd.
spellingShingle Method
Zipperlen, Peder
Nairz, Knud
Rimann, Ivo
Basler, Konrad
Hafen, Ernst
Hengartner, Michael
Hajnal, Alex
A universal method for automated gene mapping
title A universal method for automated gene mapping
title_full A universal method for automated gene mapping
title_fullStr A universal method for automated gene mapping
title_full_unstemmed A universal method for automated gene mapping
title_short A universal method for automated gene mapping
title_sort universal method for automated gene mapping
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551539/
https://www.ncbi.nlm.nih.gov/pubmed/15693948
http://dx.doi.org/10.1186/gb-2005-6-2-r19
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