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Effects of microgravity simulation on zebrafish transcriptomes and bone physiology—exposure starting at 5 days post fertilization

Physiological modifications in near weightlessness, as experienced by astronauts during space flight, have been the subject of numerous studies. Various animal models have been used on space missions or in microgravity simulation on ground to understand the effects of gravity on living animals. Here...

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Autores principales: Aceto, Jessica, Nourizadeh-Lillabadi, Rasoul, Bradamante, Silvia, Maier, Jeanette A, Alestrom, Peter, van Loon, Jack JWA, Muller, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515515/
https://www.ncbi.nlm.nih.gov/pubmed/28725727
http://dx.doi.org/10.1038/npjmgrav.2016.10
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author Aceto, Jessica
Nourizadeh-Lillabadi, Rasoul
Bradamante, Silvia
Maier, Jeanette A
Alestrom, Peter
van Loon, Jack JWA
Muller, Marc
author_facet Aceto, Jessica
Nourizadeh-Lillabadi, Rasoul
Bradamante, Silvia
Maier, Jeanette A
Alestrom, Peter
van Loon, Jack JWA
Muller, Marc
author_sort Aceto, Jessica
collection PubMed
description Physiological modifications in near weightlessness, as experienced by astronauts during space flight, have been the subject of numerous studies. Various animal models have been used on space missions or in microgravity simulation on ground to understand the effects of gravity on living animals. Here, we used the zebrafish larvae as a model to study the effect of microgravity simulation on bone formation and whole genome gene expression. To simulate microgravity (sim-μg), we used two-dimensional (2D) clinorotation starting at 5 days post fertilization to assess skeletal formation after 5 days of treatment. To assess early, regulatory effects on gene expression, a single day clinorotation was performed. Clinorotation for 5 days caused a significant decrease of bone formation, as shown by staining for cartilage and bone structures. This effect was not due to stress, as assessed by measuring cortisol levels in treated larvae. Gene expression results indicate that 1-day simulated microgravity affected musculoskeletal, cardiovascular, and nuclear receptor systems. With free-swimming model organisms such as zebrafish larvae, the 2D clinorotation setup appears to be a very appropriate approach to sim-μg. We provide evidence for alterations in bone formation and other important biological functions; in addition several affected genes and pathways involved in bone, muscle or cardiovascular development are identified.
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spelling pubmed-55155152017-07-19 Effects of microgravity simulation on zebrafish transcriptomes and bone physiology—exposure starting at 5 days post fertilization Aceto, Jessica Nourizadeh-Lillabadi, Rasoul Bradamante, Silvia Maier, Jeanette A Alestrom, Peter van Loon, Jack JWA Muller, Marc NPJ Microgravity Article Physiological modifications in near weightlessness, as experienced by astronauts during space flight, have been the subject of numerous studies. Various animal models have been used on space missions or in microgravity simulation on ground to understand the effects of gravity on living animals. Here, we used the zebrafish larvae as a model to study the effect of microgravity simulation on bone formation and whole genome gene expression. To simulate microgravity (sim-μg), we used two-dimensional (2D) clinorotation starting at 5 days post fertilization to assess skeletal formation after 5 days of treatment. To assess early, regulatory effects on gene expression, a single day clinorotation was performed. Clinorotation for 5 days caused a significant decrease of bone formation, as shown by staining for cartilage and bone structures. This effect was not due to stress, as assessed by measuring cortisol levels in treated larvae. Gene expression results indicate that 1-day simulated microgravity affected musculoskeletal, cardiovascular, and nuclear receptor systems. With free-swimming model organisms such as zebrafish larvae, the 2D clinorotation setup appears to be a very appropriate approach to sim-μg. We provide evidence for alterations in bone formation and other important biological functions; in addition several affected genes and pathways involved in bone, muscle or cardiovascular development are identified. Nature Publishing Group 2016-04-07 /pmc/articles/PMC5515515/ /pubmed/28725727 http://dx.doi.org/10.1038/npjmgrav.2016.10 Text en Copyright © 2016 Macmillan Publishers Limited http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Aceto, Jessica
Nourizadeh-Lillabadi, Rasoul
Bradamante, Silvia
Maier, Jeanette A
Alestrom, Peter
van Loon, Jack JWA
Muller, Marc
Effects of microgravity simulation on zebrafish transcriptomes and bone physiology—exposure starting at 5 days post fertilization
title Effects of microgravity simulation on zebrafish transcriptomes and bone physiology—exposure starting at 5 days post fertilization
title_full Effects of microgravity simulation on zebrafish transcriptomes and bone physiology—exposure starting at 5 days post fertilization
title_fullStr Effects of microgravity simulation on zebrafish transcriptomes and bone physiology—exposure starting at 5 days post fertilization
title_full_unstemmed Effects of microgravity simulation on zebrafish transcriptomes and bone physiology—exposure starting at 5 days post fertilization
title_short Effects of microgravity simulation on zebrafish transcriptomes and bone physiology—exposure starting at 5 days post fertilization
title_sort effects of microgravity simulation on zebrafish transcriptomes and bone physiology—exposure starting at 5 days post fertilization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515515/
https://www.ncbi.nlm.nih.gov/pubmed/28725727
http://dx.doi.org/10.1038/npjmgrav.2016.10
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