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The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy
Reorientation of cucumber seedlings induces re-localization of CsPIN1 auxin efflux carriers in endodermal cells of the transition zone between hypocotyl and roots. This study examined whether the re-localization of CsPIN1 was due to the graviresponse. Immunohistochemical analysis indicated that, whe...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515524/ https://www.ncbi.nlm.nih.gov/pubmed/28725738 http://dx.doi.org/10.1038/npjmgrav.2016.30 |
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author | Yamazaki, Chiaki Fujii, Nobuharu Miyazawa, Yutaka Kamada, Motoshi Kasahara, Haruo Osada, Ikuko Shimazu, Toru Fusejima, Yasuo Higashibata, Akira Yamazaki, Takashi Ishioka, Noriaki Takahashi, Hideyuki |
author_facet | Yamazaki, Chiaki Fujii, Nobuharu Miyazawa, Yutaka Kamada, Motoshi Kasahara, Haruo Osada, Ikuko Shimazu, Toru Fusejima, Yasuo Higashibata, Akira Yamazaki, Takashi Ishioka, Noriaki Takahashi, Hideyuki |
author_sort | Yamazaki, Chiaki |
collection | PubMed |
description | Reorientation of cucumber seedlings induces re-localization of CsPIN1 auxin efflux carriers in endodermal cells of the transition zone between hypocotyl and roots. This study examined whether the re-localization of CsPIN1 was due to the graviresponse. Immunohistochemical analysis indicated that, when cucumber seedlings were grown entirely under microgravity conditions in space, CsPIN1 in endodermal cells was mainly localized to the cell side parallel to the minor axis of the elliptic cross-section of the transition zone. However, when cucumber seeds were germinated in microgravity for 24 h and then exposed to 1g centrifugation in a direction crosswise to the seedling axis for 2 h in space, CsPIN1 was re-localized to the bottom of endodermal cells of the transition zone. These results reveal that the localization of CsPIN1 in endodermal cells changes in response to gravity. Furthermore, our results suggest that the endodermal cell layer becomes a canal by which auxin is laterally transported from the upper to the lower flank in response to gravity. The graviresponse-regulated re-localization of CsPIN1 could be responsible for the decrease in auxin level, and thus for the suppression of peg formation, on the upper side of the transition zone in horizontally placed seedlings of cucumber. |
format | Online Article Text |
id | pubmed-5515524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-55155242017-07-19 The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy Yamazaki, Chiaki Fujii, Nobuharu Miyazawa, Yutaka Kamada, Motoshi Kasahara, Haruo Osada, Ikuko Shimazu, Toru Fusejima, Yasuo Higashibata, Akira Yamazaki, Takashi Ishioka, Noriaki Takahashi, Hideyuki NPJ Microgravity Article Reorientation of cucumber seedlings induces re-localization of CsPIN1 auxin efflux carriers in endodermal cells of the transition zone between hypocotyl and roots. This study examined whether the re-localization of CsPIN1 was due to the graviresponse. Immunohistochemical analysis indicated that, when cucumber seedlings were grown entirely under microgravity conditions in space, CsPIN1 in endodermal cells was mainly localized to the cell side parallel to the minor axis of the elliptic cross-section of the transition zone. However, when cucumber seeds were germinated in microgravity for 24 h and then exposed to 1g centrifugation in a direction crosswise to the seedling axis for 2 h in space, CsPIN1 was re-localized to the bottom of endodermal cells of the transition zone. These results reveal that the localization of CsPIN1 in endodermal cells changes in response to gravity. Furthermore, our results suggest that the endodermal cell layer becomes a canal by which auxin is laterally transported from the upper to the lower flank in response to gravity. The graviresponse-regulated re-localization of CsPIN1 could be responsible for the decrease in auxin level, and thus for the suppression of peg formation, on the upper side of the transition zone in horizontally placed seedlings of cucumber. Nature Publishing Group 2016-09-15 /pmc/articles/PMC5515524/ /pubmed/28725738 http://dx.doi.org/10.1038/npjmgrav.2016.30 Text en Copyright © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Yamazaki, Chiaki Fujii, Nobuharu Miyazawa, Yutaka Kamada, Motoshi Kasahara, Haruo Osada, Ikuko Shimazu, Toru Fusejima, Yasuo Higashibata, Akira Yamazaki, Takashi Ishioka, Noriaki Takahashi, Hideyuki The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy |
title | The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy |
title_full | The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy |
title_fullStr | The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy |
title_full_unstemmed | The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy |
title_short | The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy |
title_sort | gravity-induced re-localization of auxin efflux carrier cspin1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515524/ https://www.ncbi.nlm.nih.gov/pubmed/28725738 http://dx.doi.org/10.1038/npjmgrav.2016.30 |
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