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Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape

Xanthomonas citri (Xac) is the causal agent of citrus canker, a disease that affects citrus crops and causes economic impact worldwide. To further characterize cell division in this plant pathogen, we investigated the role of the protein MinC in cell division, chromosome segregation, and peptidoglyc...

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Autores principales: Lorenzoni, André S. G., Dantas, Giordanni C., Bergsma, Tessa, Ferreira, Henrique, Scheffers, Dirk-Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515816/
https://www.ncbi.nlm.nih.gov/pubmed/28769912
http://dx.doi.org/10.3389/fmicb.2017.01352
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author Lorenzoni, André S. G.
Dantas, Giordanni C.
Bergsma, Tessa
Ferreira, Henrique
Scheffers, Dirk-Jan
author_facet Lorenzoni, André S. G.
Dantas, Giordanni C.
Bergsma, Tessa
Ferreira, Henrique
Scheffers, Dirk-Jan
author_sort Lorenzoni, André S. G.
collection PubMed
description Xanthomonas citri (Xac) is the causal agent of citrus canker, a disease that affects citrus crops and causes economic impact worldwide. To further characterize cell division in this plant pathogen, we investigated the role of the protein MinC in cell division, chromosome segregation, and peptidoglycan incorporation by deleting the gene minC using allele exchange. Xac with minC deleted exhibited the classic Δmin phenotype observed in other bacteria deleted for min components: minicells and short filamentation. In addition we noticed the formation of branches, which is similar to what was previously described for Escherichia coli deleted for either min or for several low molecular weight penicillin-binding proteins (PBPs). The branching phenotype was medium dependent and probably linked to gluconeogenic growth. We complemented the minC gene by integrating gfp-minC into the amy locus. Xac complemented strains displayed a wild-type phenotype. In addition, GFP-MinC oscillated from pole to pole, similar to MinCD oscillations observed in E. coli and more recently in Synechococcus elongatus. Further investigation of the branching phenotype revealed that in branching cells nucleoid organization, divisome formation and peptidoglycan incorporation were disrupted.
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spelling pubmed-55158162017-08-02 Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape Lorenzoni, André S. G. Dantas, Giordanni C. Bergsma, Tessa Ferreira, Henrique Scheffers, Dirk-Jan Front Microbiol Microbiology Xanthomonas citri (Xac) is the causal agent of citrus canker, a disease that affects citrus crops and causes economic impact worldwide. To further characterize cell division in this plant pathogen, we investigated the role of the protein MinC in cell division, chromosome segregation, and peptidoglycan incorporation by deleting the gene minC using allele exchange. Xac with minC deleted exhibited the classic Δmin phenotype observed in other bacteria deleted for min components: minicells and short filamentation. In addition we noticed the formation of branches, which is similar to what was previously described for Escherichia coli deleted for either min or for several low molecular weight penicillin-binding proteins (PBPs). The branching phenotype was medium dependent and probably linked to gluconeogenic growth. We complemented the minC gene by integrating gfp-minC into the amy locus. Xac complemented strains displayed a wild-type phenotype. In addition, GFP-MinC oscillated from pole to pole, similar to MinCD oscillations observed in E. coli and more recently in Synechococcus elongatus. Further investigation of the branching phenotype revealed that in branching cells nucleoid organization, divisome formation and peptidoglycan incorporation were disrupted. Frontiers Media S.A. 2017-07-19 /pmc/articles/PMC5515816/ /pubmed/28769912 http://dx.doi.org/10.3389/fmicb.2017.01352 Text en Copyright © 2017 Lorenzoni, Dantas, Bergsma, Ferreira and Scheffers. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lorenzoni, André S. G.
Dantas, Giordanni C.
Bergsma, Tessa
Ferreira, Henrique
Scheffers, Dirk-Jan
Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape
title Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape
title_full Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape
title_fullStr Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape
title_full_unstemmed Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape
title_short Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape
title_sort xanthomonas citri minc oscillates from pole to pole to ensure proper cell division and shape
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515816/
https://www.ncbi.nlm.nih.gov/pubmed/28769912
http://dx.doi.org/10.3389/fmicb.2017.01352
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