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A Combination of Ex vivo Diffusion MRI and Multiphoton to Study Microglia/Monocytes Alterations after Spinal Cord Injury

Central nervous system (CNS) injury has been observed to lead to microglia activation and monocytes infiltration at the lesion site. Ex vivo diffusion magnetic resonance imaging (diffusion MRI or DWI) allows detailed examination of CNS tissues, and recent advances in clearing procedures allow detail...

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Autores principales: Noristani, Harun N., Boukhaddaoui, Hassan, Saint-Martin, Guillaume, Auzer, Pauline, Sidiboulenouar, Rahima, Lonjon, Nicolas, Alibert, Eric, Tricaud, Nicolas, Goze-Bac, Christophe, Coillot, Christophe, Perrin, Florence E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515855/
https://www.ncbi.nlm.nih.gov/pubmed/28769787
http://dx.doi.org/10.3389/fnagi.2017.00230
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author Noristani, Harun N.
Boukhaddaoui, Hassan
Saint-Martin, Guillaume
Auzer, Pauline
Sidiboulenouar, Rahima
Lonjon, Nicolas
Alibert, Eric
Tricaud, Nicolas
Goze-Bac, Christophe
Coillot, Christophe
Perrin, Florence E.
author_facet Noristani, Harun N.
Boukhaddaoui, Hassan
Saint-Martin, Guillaume
Auzer, Pauline
Sidiboulenouar, Rahima
Lonjon, Nicolas
Alibert, Eric
Tricaud, Nicolas
Goze-Bac, Christophe
Coillot, Christophe
Perrin, Florence E.
author_sort Noristani, Harun N.
collection PubMed
description Central nervous system (CNS) injury has been observed to lead to microglia activation and monocytes infiltration at the lesion site. Ex vivo diffusion magnetic resonance imaging (diffusion MRI or DWI) allows detailed examination of CNS tissues, and recent advances in clearing procedures allow detailed imaging of fluorescent-labeled cells at high resolution. No study has yet combined ex vivo diffusion MRI and clearing procedures to establish a possible link between microglia/monocytes response and diffusion coefficient in the context of spinal cord injury (SCI). We carried out ex vivo MRI of the spinal cord at different time-points after spinal cord transection followed by tetrahydrofuran based clearing and examined the density and morphology of microglia/monocytes using two-photon microscopy. Quantitative analysis revealed an early marked increase in microglial/monocytes density that is associated with an increase in the extension of the lesion measured using diffusion MRI. Morphological examination of microglia/monocytes somata at the lesion site revealed a significant increase in their surface area and volume as early as 72 hours post-injury. Time-course analysis showed differential microglial/monocytes response rostral and caudal to the lesion site. Microglia/monocytes showed a decrease in reactivity over time caudal to the lesion site, but an increase was observed rostrally. Direct comparison of microglia/monocytes morphology, obtained through multiphoton, and the longitudinal apparent diffusion coefficient (ADC), measured with diffusion MRI, highlighted that axonal integrity does not correlate with the density of microglia/monocytes or their somata morphology. We emphasize that differential microglial/monocytes reactivity rostral and caudal to the lesion site may thus coincide, at least partially, with reported temporal differences in debris clearance. Our study demonstrates that the combination of ex vivo diffusion MRI and two-photon microscopy may be used to follow structural tissue alteration. Lesion extension coincides with microglia/monocytes density; however, a direct relationship between ADC and microglia/monocytes density and morphology was not observed. We highlighted a differential rostro-caudal microglia/monocytes reactivity that may correspond to a temporal difference in debris clearance and axonal integrity. Thus, potential therapeutic strategies targeting microglia/monocytes after SCI may need to be adjusted not only with the time after injury but also relative to the location to the lesion site.
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spelling pubmed-55158552017-08-02 A Combination of Ex vivo Diffusion MRI and Multiphoton to Study Microglia/Monocytes Alterations after Spinal Cord Injury Noristani, Harun N. Boukhaddaoui, Hassan Saint-Martin, Guillaume Auzer, Pauline Sidiboulenouar, Rahima Lonjon, Nicolas Alibert, Eric Tricaud, Nicolas Goze-Bac, Christophe Coillot, Christophe Perrin, Florence E. Front Aging Neurosci Neuroscience Central nervous system (CNS) injury has been observed to lead to microglia activation and monocytes infiltration at the lesion site. Ex vivo diffusion magnetic resonance imaging (diffusion MRI or DWI) allows detailed examination of CNS tissues, and recent advances in clearing procedures allow detailed imaging of fluorescent-labeled cells at high resolution. No study has yet combined ex vivo diffusion MRI and clearing procedures to establish a possible link between microglia/monocytes response and diffusion coefficient in the context of spinal cord injury (SCI). We carried out ex vivo MRI of the spinal cord at different time-points after spinal cord transection followed by tetrahydrofuran based clearing and examined the density and morphology of microglia/monocytes using two-photon microscopy. Quantitative analysis revealed an early marked increase in microglial/monocytes density that is associated with an increase in the extension of the lesion measured using diffusion MRI. Morphological examination of microglia/monocytes somata at the lesion site revealed a significant increase in their surface area and volume as early as 72 hours post-injury. Time-course analysis showed differential microglial/monocytes response rostral and caudal to the lesion site. Microglia/monocytes showed a decrease in reactivity over time caudal to the lesion site, but an increase was observed rostrally. Direct comparison of microglia/monocytes morphology, obtained through multiphoton, and the longitudinal apparent diffusion coefficient (ADC), measured with diffusion MRI, highlighted that axonal integrity does not correlate with the density of microglia/monocytes or their somata morphology. We emphasize that differential microglial/monocytes reactivity rostral and caudal to the lesion site may thus coincide, at least partially, with reported temporal differences in debris clearance. Our study demonstrates that the combination of ex vivo diffusion MRI and two-photon microscopy may be used to follow structural tissue alteration. Lesion extension coincides with microglia/monocytes density; however, a direct relationship between ADC and microglia/monocytes density and morphology was not observed. We highlighted a differential rostro-caudal microglia/monocytes reactivity that may correspond to a temporal difference in debris clearance and axonal integrity. Thus, potential therapeutic strategies targeting microglia/monocytes after SCI may need to be adjusted not only with the time after injury but also relative to the location to the lesion site. Frontiers Media S.A. 2017-07-19 /pmc/articles/PMC5515855/ /pubmed/28769787 http://dx.doi.org/10.3389/fnagi.2017.00230 Text en Copyright © 2017 Noristani, Boukhaddaoui, Saint-Martin, Auzer, Sidiboulenouar, Lonjon, Alibert, Tricaud, Goze-Bac, Coillot and Perrin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Noristani, Harun N.
Boukhaddaoui, Hassan
Saint-Martin, Guillaume
Auzer, Pauline
Sidiboulenouar, Rahima
Lonjon, Nicolas
Alibert, Eric
Tricaud, Nicolas
Goze-Bac, Christophe
Coillot, Christophe
Perrin, Florence E.
A Combination of Ex vivo Diffusion MRI and Multiphoton to Study Microglia/Monocytes Alterations after Spinal Cord Injury
title A Combination of Ex vivo Diffusion MRI and Multiphoton to Study Microglia/Monocytes Alterations after Spinal Cord Injury
title_full A Combination of Ex vivo Diffusion MRI and Multiphoton to Study Microglia/Monocytes Alterations after Spinal Cord Injury
title_fullStr A Combination of Ex vivo Diffusion MRI and Multiphoton to Study Microglia/Monocytes Alterations after Spinal Cord Injury
title_full_unstemmed A Combination of Ex vivo Diffusion MRI and Multiphoton to Study Microglia/Monocytes Alterations after Spinal Cord Injury
title_short A Combination of Ex vivo Diffusion MRI and Multiphoton to Study Microglia/Monocytes Alterations after Spinal Cord Injury
title_sort combination of ex vivo diffusion mri and multiphoton to study microglia/monocytes alterations after spinal cord injury
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515855/
https://www.ncbi.nlm.nih.gov/pubmed/28769787
http://dx.doi.org/10.3389/fnagi.2017.00230
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