Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis

Most analyzed Lactococcus lactis strains are predicted to harbor one or more prophage genomes within their chromosome; however, the true extent of the inducibility and functionality of such prophages cannot easily be deduced from sequence analysis alone. Chemical treatment of lysogenic strains with...

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Autores principales: Oliveira, Joana, Mahony, Jennifer, Hanemaaijer, Laurens, Kouwen, Thijs R. H. M., Neve, Horst, MacSharry, John, van Sinderen, Douwe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515857/
https://www.ncbi.nlm.nih.gov/pubmed/28769907
http://dx.doi.org/10.3389/fmicb.2017.01343
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author Oliveira, Joana
Mahony, Jennifer
Hanemaaijer, Laurens
Kouwen, Thijs R. H. M.
Neve, Horst
MacSharry, John
van Sinderen, Douwe
author_facet Oliveira, Joana
Mahony, Jennifer
Hanemaaijer, Laurens
Kouwen, Thijs R. H. M.
Neve, Horst
MacSharry, John
van Sinderen, Douwe
author_sort Oliveira, Joana
collection PubMed
description Most analyzed Lactococcus lactis strains are predicted to harbor one or more prophage genomes within their chromosome; however, the true extent of the inducibility and functionality of such prophages cannot easily be deduced from sequence analysis alone. Chemical treatment of lysogenic strains with Mitomycin C is known to cause induction of temperate phages, though it is not always easy to clearly identify a lysogenic strain or to measure the number of released phage particles. Here, we report the application of flow cytometry as a reliable tool for the detection and enumeration of released lactococcal prophages using the green dye SYTO-9.
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spelling pubmed-55158572017-08-02 Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis Oliveira, Joana Mahony, Jennifer Hanemaaijer, Laurens Kouwen, Thijs R. H. M. Neve, Horst MacSharry, John van Sinderen, Douwe Front Microbiol Microbiology Most analyzed Lactococcus lactis strains are predicted to harbor one or more prophage genomes within their chromosome; however, the true extent of the inducibility and functionality of such prophages cannot easily be deduced from sequence analysis alone. Chemical treatment of lysogenic strains with Mitomycin C is known to cause induction of temperate phages, though it is not always easy to clearly identify a lysogenic strain or to measure the number of released phage particles. Here, we report the application of flow cytometry as a reliable tool for the detection and enumeration of released lactococcal prophages using the green dye SYTO-9. Frontiers Media S.A. 2017-07-19 /pmc/articles/PMC5515857/ /pubmed/28769907 http://dx.doi.org/10.3389/fmicb.2017.01343 Text en Copyright © 2017 Oliveira, Mahony, Hanemaaijer, Kouwen, Neve, MacSharry and van Sinderen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Oliveira, Joana
Mahony, Jennifer
Hanemaaijer, Laurens
Kouwen, Thijs R. H. M.
Neve, Horst
MacSharry, John
van Sinderen, Douwe
Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis
title Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis
title_full Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis
title_fullStr Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis
title_full_unstemmed Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis
title_short Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis
title_sort detecting lactococcus lactis prophages by mitomycin c-mediated induction coupled to flow cytometry analysis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515857/
https://www.ncbi.nlm.nih.gov/pubmed/28769907
http://dx.doi.org/10.3389/fmicb.2017.01343
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