A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation

The present work aims to develop a growth medium to render a wild-type strain of Saccharomyces cerevisiae permeable to the antifungal drug Brefeldin A. In the current study, a synthetic medium containing 0.1% L-proline and supplemented with 3.0 × 10(-3)% SDS is employed. When Brefeldin A is added to...

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Detalles Bibliográficos
Autores principales: Pannunzio, Vanesa G., Burgos, Hilda I., Alonso, Manuel, Mattoon, James R., Ramos, Eugenia H., Stella, Carlos A.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2004
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551586/
https://www.ncbi.nlm.nih.gov/pubmed/15292581
http://dx.doi.org/10.1155/S1110724304308077
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author Pannunzio, Vanesa G.
Burgos, Hilda I.
Alonso, Manuel
Mattoon, James R.
Ramos, Eugenia H.
Stella, Carlos A.
author_facet Pannunzio, Vanesa G.
Burgos, Hilda I.
Alonso, Manuel
Mattoon, James R.
Ramos, Eugenia H.
Stella, Carlos A.
author_sort Pannunzio, Vanesa G.
collection PubMed
description The present work aims to develop a growth medium to render a wild-type strain of Saccharomyces cerevisiae permeable to the antifungal drug Brefeldin A. In the current study, a synthetic medium containing 0.1% L-proline and supplemented with 3.0 × 10(-3)% SDS is employed. When Brefeldin A is added to this medium, a wild-type strain shows increased growth sensitivity and a diminished transport of the amino acid L-leucine. Since Brefeldin A exerts its effect on the endoplasmic reticulum and the Golgi apparatus, the medium permits the study of the drug effect on the intracellular traffic of L-leucine permeases.
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spelling pubmed-5515862005-04-05 A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation Pannunzio, Vanesa G. Burgos, Hilda I. Alonso, Manuel Mattoon, James R. Ramos, Eugenia H. Stella, Carlos A. J Biomed Biotechnol Research Article The present work aims to develop a growth medium to render a wild-type strain of Saccharomyces cerevisiae permeable to the antifungal drug Brefeldin A. In the current study, a synthetic medium containing 0.1% L-proline and supplemented with 3.0 × 10(-3)% SDS is employed. When Brefeldin A is added to this medium, a wild-type strain shows increased growth sensitivity and a diminished transport of the amino acid L-leucine. Since Brefeldin A exerts its effect on the endoplasmic reticulum and the Golgi apparatus, the medium permits the study of the drug effect on the intracellular traffic of L-leucine permeases. Hindawi Publishing Corporation 2004-07-29 /pmc/articles/PMC551586/ /pubmed/15292581 http://dx.doi.org/10.1155/S1110724304308077 Text en Hindawi Publishing Corporation
spellingShingle Research Article
Pannunzio, Vanesa G.
Burgos, Hilda I.
Alonso, Manuel
Mattoon, James R.
Ramos, Eugenia H.
Stella, Carlos A.
A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation
title A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation
title_full A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation
title_fullStr A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation
title_full_unstemmed A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation
title_short A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation
title_sort simple chemical method for rendering wild-type yeast permeable to brefeldin a that does not require the presence of an erg6 mutation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551586/
https://www.ncbi.nlm.nih.gov/pubmed/15292581
http://dx.doi.org/10.1155/S1110724304308077
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