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Mimicking Paracrine TGFβ1 Signals during Myofibroblast Differentiation in 3D Collagen Networks

TGFβ1 is a key regulator for induction of tissue remodeling after dermal wounding. We present a model of paracrine delivery of TGFβ1 for differentiation of dermal fibroblasts based on a fibrillar 3D collagen matrix and embedded TGFβ1 releasing microparticles. We found differentiation into myofibrobl...

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Detalles Bibliográficos
Autores principales: Ansorge, Michael, Sapudom, Jiranuwat, Chkolnikov, Marina, Wilde, Martin, Anderegg, Ulf, Möller, Stephanie, Schnabelrauch, Matthias, Pompe, Tilo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515936/
https://www.ncbi.nlm.nih.gov/pubmed/28720779
http://dx.doi.org/10.1038/s41598-017-05912-x
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author Ansorge, Michael
Sapudom, Jiranuwat
Chkolnikov, Marina
Wilde, Martin
Anderegg, Ulf
Möller, Stephanie
Schnabelrauch, Matthias
Pompe, Tilo
author_facet Ansorge, Michael
Sapudom, Jiranuwat
Chkolnikov, Marina
Wilde, Martin
Anderegg, Ulf
Möller, Stephanie
Schnabelrauch, Matthias
Pompe, Tilo
author_sort Ansorge, Michael
collection PubMed
description TGFβ1 is a key regulator for induction of tissue remodeling after dermal wounding. We present a model of paracrine delivery of TGFβ1 for differentiation of dermal fibroblasts based on a fibrillar 3D collagen matrix and embedded TGFβ1 releasing microparticles. We found differentiation into myofibroblasts was achieved in a TGFβ1 dependent manner at much lower doses than systemic delivery. This effect is accounted to the slow and sustained TGFβ1 release mimicking paracrine cell signals.
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spelling pubmed-55159362017-07-19 Mimicking Paracrine TGFβ1 Signals during Myofibroblast Differentiation in 3D Collagen Networks Ansorge, Michael Sapudom, Jiranuwat Chkolnikov, Marina Wilde, Martin Anderegg, Ulf Möller, Stephanie Schnabelrauch, Matthias Pompe, Tilo Sci Rep Article TGFβ1 is a key regulator for induction of tissue remodeling after dermal wounding. We present a model of paracrine delivery of TGFβ1 for differentiation of dermal fibroblasts based on a fibrillar 3D collagen matrix and embedded TGFβ1 releasing microparticles. We found differentiation into myofibroblasts was achieved in a TGFβ1 dependent manner at much lower doses than systemic delivery. This effect is accounted to the slow and sustained TGFβ1 release mimicking paracrine cell signals. Nature Publishing Group UK 2017-07-18 /pmc/articles/PMC5515936/ /pubmed/28720779 http://dx.doi.org/10.1038/s41598-017-05912-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ansorge, Michael
Sapudom, Jiranuwat
Chkolnikov, Marina
Wilde, Martin
Anderegg, Ulf
Möller, Stephanie
Schnabelrauch, Matthias
Pompe, Tilo
Mimicking Paracrine TGFβ1 Signals during Myofibroblast Differentiation in 3D Collagen Networks
title Mimicking Paracrine TGFβ1 Signals during Myofibroblast Differentiation in 3D Collagen Networks
title_full Mimicking Paracrine TGFβ1 Signals during Myofibroblast Differentiation in 3D Collagen Networks
title_fullStr Mimicking Paracrine TGFβ1 Signals during Myofibroblast Differentiation in 3D Collagen Networks
title_full_unstemmed Mimicking Paracrine TGFβ1 Signals during Myofibroblast Differentiation in 3D Collagen Networks
title_short Mimicking Paracrine TGFβ1 Signals during Myofibroblast Differentiation in 3D Collagen Networks
title_sort mimicking paracrine tgfβ1 signals during myofibroblast differentiation in 3d collagen networks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515936/
https://www.ncbi.nlm.nih.gov/pubmed/28720779
http://dx.doi.org/10.1038/s41598-017-05912-x
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