Cargando…

The advent of structural biology in situ by single particle cryo-electron tomography

Single particle tomography (SPT), also known as subtomogram averaging, is a powerful technique uniquely poised to address questions in structural biology that are not amenable to more traditional approaches like X-ray crystallography, nuclear magnetic resonance, and conventional cryoEM single partic...

Descripción completa

Detalles Bibliográficos
Autores principales: Galaz-Montoya, Jesús G., Ludtke, Steven J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516000/
https://www.ncbi.nlm.nih.gov/pubmed/28781998
http://dx.doi.org/10.1007/s41048-017-0040-0
_version_ 1783251077043322880
author Galaz-Montoya, Jesús G.
Ludtke, Steven J.
author_facet Galaz-Montoya, Jesús G.
Ludtke, Steven J.
author_sort Galaz-Montoya, Jesús G.
collection PubMed
description Single particle tomography (SPT), also known as subtomogram averaging, is a powerful technique uniquely poised to address questions in structural biology that are not amenable to more traditional approaches like X-ray crystallography, nuclear magnetic resonance, and conventional cryoEM single particle analysis. Owing to its potential for in situ structural biology at subnanometer resolution, SPT has been gaining enormous momentum in the last five years and is becoming a prominent, widely used technique. This method can be applied to unambiguously determine the structures of macromolecular complexes that exhibit compositional and conformational heterogeneity, both in vitro and in situ. Here we review the development of SPT, highlighting its applications and identifying areas of ongoing development.
format Online
Article
Text
id pubmed-5516000
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-55160002017-08-02 The advent of structural biology in situ by single particle cryo-electron tomography Galaz-Montoya, Jesús G. Ludtke, Steven J. Biophys Rep Method Single particle tomography (SPT), also known as subtomogram averaging, is a powerful technique uniquely poised to address questions in structural biology that are not amenable to more traditional approaches like X-ray crystallography, nuclear magnetic resonance, and conventional cryoEM single particle analysis. Owing to its potential for in situ structural biology at subnanometer resolution, SPT has been gaining enormous momentum in the last five years and is becoming a prominent, widely used technique. This method can be applied to unambiguously determine the structures of macromolecular complexes that exhibit compositional and conformational heterogeneity, both in vitro and in situ. Here we review the development of SPT, highlighting its applications and identifying areas of ongoing development. Springer Berlin Heidelberg 2017-05-29 2017 /pmc/articles/PMC5516000/ /pubmed/28781998 http://dx.doi.org/10.1007/s41048-017-0040-0 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Method
Galaz-Montoya, Jesús G.
Ludtke, Steven J.
The advent of structural biology in situ by single particle cryo-electron tomography
title The advent of structural biology in situ by single particle cryo-electron tomography
title_full The advent of structural biology in situ by single particle cryo-electron tomography
title_fullStr The advent of structural biology in situ by single particle cryo-electron tomography
title_full_unstemmed The advent of structural biology in situ by single particle cryo-electron tomography
title_short The advent of structural biology in situ by single particle cryo-electron tomography
title_sort advent of structural biology in situ by single particle cryo-electron tomography
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516000/
https://www.ncbi.nlm.nih.gov/pubmed/28781998
http://dx.doi.org/10.1007/s41048-017-0040-0
work_keys_str_mv AT galazmontoyajesusg theadventofstructuralbiologyinsitubysingleparticlecryoelectrontomography
AT ludtkestevenj theadventofstructuralbiologyinsitubysingleparticlecryoelectrontomography
AT galazmontoyajesusg adventofstructuralbiologyinsitubysingleparticlecryoelectrontomography
AT ludtkestevenj adventofstructuralbiologyinsitubysingleparticlecryoelectrontomography