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Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3)

While investigating biomarkers for infection with salmonid alphavirus (SAV), the cause of pancreas disease (PD), a selective precipitation reaction (SPR) has been discovered in serum which could be an on‐farm qualitative test and an in‐laboratory quantitative assay for health assessments in aquacult...

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Autores principales: Braceland, M, Tinsley, J, Cockerill, D, Bickerdike, R, McLoughlin, M F, Eckersall, P D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516131/
https://www.ncbi.nlm.nih.gov/pubmed/27905123
http://dx.doi.org/10.1111/jfd.12587
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author Braceland, M
Tinsley, J
Cockerill, D
Bickerdike, R
McLoughlin, M F
Eckersall, P D
author_facet Braceland, M
Tinsley, J
Cockerill, D
Bickerdike, R
McLoughlin, M F
Eckersall, P D
author_sort Braceland, M
collection PubMed
description While investigating biomarkers for infection with salmonid alphavirus (SAV), the cause of pancreas disease (PD), a selective precipitation reaction (SPR) has been discovered in serum which could be an on‐farm qualitative test and an in‐laboratory quantitative assay for health assessments in aquaculture. Mixing serum from Atlantic salmon, Salmo salar, with SAV infection with a sodium acetate buffer caused a visible precipitation which does not occur with serum from healthy salmon. Proteomic examination of the precipitate has revealed that the components are a mix of muscle proteins, for example enolase and aldolase, along with serum protein such as serotransferrin and complement C9. The assay has been optimized for molarity, pH, temperature and wavelength so that the precipitation can be measured as the change in optical density at 340 nm (Δ(340)). Application of the SPR assay to serum samples from a cohabitation trial of SAV infection in salmon showed that the Δ(340) in infected fish rose from undetectable to a maximum at 6 weeks post‐infection correlating with histopathological score of pancreas, heart and muscle damage. This test may have a valuable role to play in the diagnostic evaluation of stock health in salmon.
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spelling pubmed-55161312017-08-02 Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3) Braceland, M Tinsley, J Cockerill, D Bickerdike, R McLoughlin, M F Eckersall, P D J Fish Dis Original Articles While investigating biomarkers for infection with salmonid alphavirus (SAV), the cause of pancreas disease (PD), a selective precipitation reaction (SPR) has been discovered in serum which could be an on‐farm qualitative test and an in‐laboratory quantitative assay for health assessments in aquaculture. Mixing serum from Atlantic salmon, Salmo salar, with SAV infection with a sodium acetate buffer caused a visible precipitation which does not occur with serum from healthy salmon. Proteomic examination of the precipitate has revealed that the components are a mix of muscle proteins, for example enolase and aldolase, along with serum protein such as serotransferrin and complement C9. The assay has been optimized for molarity, pH, temperature and wavelength so that the precipitation can be measured as the change in optical density at 340 nm (Δ(340)). Application of the SPR assay to serum samples from a cohabitation trial of SAV infection in salmon showed that the Δ(340) in infected fish rose from undetectable to a maximum at 6 weeks post‐infection correlating with histopathological score of pancreas, heart and muscle damage. This test may have a valuable role to play in the diagnostic evaluation of stock health in salmon. John Wiley and Sons Inc. 2016-11-30 2017-08 /pmc/articles/PMC5516131/ /pubmed/27905123 http://dx.doi.org/10.1111/jfd.12587 Text en © 2016 The Authors. Journal of Fish Diseases Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Braceland, M
Tinsley, J
Cockerill, D
Bickerdike, R
McLoughlin, M F
Eckersall, P D
Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3)
title Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3)
title_full Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3)
title_fullStr Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3)
title_full_unstemmed Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3)
title_short Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3)
title_sort selective precipitation reaction: a novel diagnostic test for tissue pathology in atlantic salmon, salmo salar, infected with salmonid alphavirus (sav3)
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516131/
https://www.ncbi.nlm.nih.gov/pubmed/27905123
http://dx.doi.org/10.1111/jfd.12587
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