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Gene expression atlas for the food security crop cassava

Cassava (Manihot esculenta) feeds c. 800 million people world‐wide. Although this crop displays high productivity under drought and poor soil conditions, it is susceptible to disease, postharvest deterioration and the roots contain low nutritional content. Here, we provide molecular identities for 1...

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Autores principales: Wilson, Mark C., Mutka, Andrew M., Hummel, Aaron W., Berry, Jeffrey, Chauhan, Raj Deepika, Vijayaraghavan, Anupama, Taylor, Nigel J., Voytas, Daniel F., Chitwood, Daniel H., Bart, Rebecca S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516207/
https://www.ncbi.nlm.nih.gov/pubmed/28116755
http://dx.doi.org/10.1111/nph.14443
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author Wilson, Mark C.
Mutka, Andrew M.
Hummel, Aaron W.
Berry, Jeffrey
Chauhan, Raj Deepika
Vijayaraghavan, Anupama
Taylor, Nigel J.
Voytas, Daniel F.
Chitwood, Daniel H.
Bart, Rebecca S.
author_facet Wilson, Mark C.
Mutka, Andrew M.
Hummel, Aaron W.
Berry, Jeffrey
Chauhan, Raj Deepika
Vijayaraghavan, Anupama
Taylor, Nigel J.
Voytas, Daniel F.
Chitwood, Daniel H.
Bart, Rebecca S.
author_sort Wilson, Mark C.
collection PubMed
description Cassava (Manihot esculenta) feeds c. 800 million people world‐wide. Although this crop displays high productivity under drought and poor soil conditions, it is susceptible to disease, postharvest deterioration and the roots contain low nutritional content. Here, we provide molecular identities for 11 cassava tissue/organ types through RNA‐sequencing and develop an open access, web‐based interface for further interrogation of the data. Through this dataset, we consider the physiology of cassava. Specifically, we focus on identification of the transcriptional signatures that define the massive, underground storage roots used as a food source and the favored target tissue for transgene integration and genome editing, friable embryogenic callus (FEC). Further, we identify promoters able to drive strong expression in multiple tissue/organs. The information gained from this study is of value for both conventional and biotechnological improvement programs.
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spelling pubmed-55162072017-08-02 Gene expression atlas for the food security crop cassava Wilson, Mark C. Mutka, Andrew M. Hummel, Aaron W. Berry, Jeffrey Chauhan, Raj Deepika Vijayaraghavan, Anupama Taylor, Nigel J. Voytas, Daniel F. Chitwood, Daniel H. Bart, Rebecca S. New Phytol Research Cassava (Manihot esculenta) feeds c. 800 million people world‐wide. Although this crop displays high productivity under drought and poor soil conditions, it is susceptible to disease, postharvest deterioration and the roots contain low nutritional content. Here, we provide molecular identities for 11 cassava tissue/organ types through RNA‐sequencing and develop an open access, web‐based interface for further interrogation of the data. Through this dataset, we consider the physiology of cassava. Specifically, we focus on identification of the transcriptional signatures that define the massive, underground storage roots used as a food source and the favored target tissue for transgene integration and genome editing, friable embryogenic callus (FEC). Further, we identify promoters able to drive strong expression in multiple tissue/organs. The information gained from this study is of value for both conventional and biotechnological improvement programs. John Wiley and Sons Inc. 2017-01-24 2017-03 /pmc/articles/PMC5516207/ /pubmed/28116755 http://dx.doi.org/10.1111/nph.14443 Text en © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Wilson, Mark C.
Mutka, Andrew M.
Hummel, Aaron W.
Berry, Jeffrey
Chauhan, Raj Deepika
Vijayaraghavan, Anupama
Taylor, Nigel J.
Voytas, Daniel F.
Chitwood, Daniel H.
Bart, Rebecca S.
Gene expression atlas for the food security crop cassava
title Gene expression atlas for the food security crop cassava
title_full Gene expression atlas for the food security crop cassava
title_fullStr Gene expression atlas for the food security crop cassava
title_full_unstemmed Gene expression atlas for the food security crop cassava
title_short Gene expression atlas for the food security crop cassava
title_sort gene expression atlas for the food security crop cassava
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516207/
https://www.ncbi.nlm.nih.gov/pubmed/28116755
http://dx.doi.org/10.1111/nph.14443
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