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Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway
Our aim was to investigate the effects of phycocyanin (PC) on bleomycin (BLM)-induced pulmonary fibrosis (PF). In this study, C57 BL/6 wild-type (WT) mice and toll-like receptor (TLR) 2 deficient mice were treated with PC for 28 days following BLM exposure. Serum and lung tissues were collected on d...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5517415/ https://www.ncbi.nlm.nih.gov/pubmed/28725012 http://dx.doi.org/10.1038/s41598-017-06021-5 |
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author | Li, Chengcheng Yu, Yan Li, Wenjun Liu, Bo Jiao, Xudong Song, Xinyu Lv, Changjun Qin, Song |
author_facet | Li, Chengcheng Yu, Yan Li, Wenjun Liu, Bo Jiao, Xudong Song, Xinyu Lv, Changjun Qin, Song |
author_sort | Li, Chengcheng |
collection | PubMed |
description | Our aim was to investigate the effects of phycocyanin (PC) on bleomycin (BLM)-induced pulmonary fibrosis (PF). In this study, C57 BL/6 wild-type (WT) mice and toll-like receptor (TLR) 2 deficient mice were treated with PC for 28 days following BLM exposure. Serum and lung tissues were collected on days 3, 7 and 28. Data shows PC significantly decreased the levels of hydroxyproline (HYP), vimentin, surfactant-associated protein C (SP-C), fibroblast specific protein-1 (S100A4) and α-smooth muscle actin (α-SMA) but dramatically increased E-cadherin and podoplanin (PDPN) expression on day 28. Moreover, PC greatly decreased the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and myeloperoxidase (MPO) at the earlier time. Reduced expression of key genes in the TLR2 pathway was also detected. Compared with WT mice, TLR2-deficient mice exhibited less injury, and the protective effect of PC was partly diminished in this background. These data indicate the anti-fibrotic effects of PC may be mediated by reducing W/D ratio, MPO, IL-6, TNF-α, protecting type I alveolar epithelial cells, inhibiting fibroblast proliferation, attenuating epithelial-mesenchymal transitions (EMT) and reducing oxidative stress. The TLR2-MyD88-NF-κB pathway plays an important role in PC-mediated reduction in pulmonary fibrosis. |
format | Online Article Text |
id | pubmed-5517415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55174152017-07-20 Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway Li, Chengcheng Yu, Yan Li, Wenjun Liu, Bo Jiao, Xudong Song, Xinyu Lv, Changjun Qin, Song Sci Rep Article Our aim was to investigate the effects of phycocyanin (PC) on bleomycin (BLM)-induced pulmonary fibrosis (PF). In this study, C57 BL/6 wild-type (WT) mice and toll-like receptor (TLR) 2 deficient mice were treated with PC for 28 days following BLM exposure. Serum and lung tissues were collected on days 3, 7 and 28. Data shows PC significantly decreased the levels of hydroxyproline (HYP), vimentin, surfactant-associated protein C (SP-C), fibroblast specific protein-1 (S100A4) and α-smooth muscle actin (α-SMA) but dramatically increased E-cadherin and podoplanin (PDPN) expression on day 28. Moreover, PC greatly decreased the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and myeloperoxidase (MPO) at the earlier time. Reduced expression of key genes in the TLR2 pathway was also detected. Compared with WT mice, TLR2-deficient mice exhibited less injury, and the protective effect of PC was partly diminished in this background. These data indicate the anti-fibrotic effects of PC may be mediated by reducing W/D ratio, MPO, IL-6, TNF-α, protecting type I alveolar epithelial cells, inhibiting fibroblast proliferation, attenuating epithelial-mesenchymal transitions (EMT) and reducing oxidative stress. The TLR2-MyD88-NF-κB pathway plays an important role in PC-mediated reduction in pulmonary fibrosis. Nature Publishing Group UK 2017-07-19 /pmc/articles/PMC5517415/ /pubmed/28725012 http://dx.doi.org/10.1038/s41598-017-06021-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Li, Chengcheng Yu, Yan Li, Wenjun Liu, Bo Jiao, Xudong Song, Xinyu Lv, Changjun Qin, Song Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway |
title | Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway |
title_full | Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway |
title_fullStr | Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway |
title_full_unstemmed | Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway |
title_short | Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway |
title_sort | phycocyanin attenuates pulmonary fibrosis via the tlr2-myd88-nf-κb signaling pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5517415/ https://www.ncbi.nlm.nih.gov/pubmed/28725012 http://dx.doi.org/10.1038/s41598-017-06021-5 |
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